A novel therapeutic vaccine of GM-CSF/TNF[alpha] surface-modified RM-1 cells against the orthotopic prostatic cancer
A novel therapeutic vaccine against prostate cancer was developed by simultaneous immobilization of streptavidin-tagged bioactive GM-CSF and TNFα on the biotinylated surface of 30% ethanol-fixed RM-1 prostatic cancer cells. This study showed that the GM-CSF/TNFα-doubly surface-modified vaccine signi...
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| Veröffentlicht in: | Vaccine 2010-07, Vol.28 (31), p.4937 |
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| container_issue | 31 |
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| container_title | Vaccine |
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| creator | Yin, Weihua He, Qiushan Hu, Zhiming Chen, Zhong Qifeng, Mao Zhichun, Song Zhihui, Qu Xiaoxia, Nie Li, Jinlong Gao, Jimin |
| description | A novel therapeutic vaccine against prostate cancer was developed by simultaneous immobilization of streptavidin-tagged bioactive GM-CSF and TNFα on the biotinylated surface of 30% ethanol-fixed RM-1 prostatic cancer cells. This study showed that the GM-CSF/TNFα-doubly surface-modified vaccine significantly extended the survival in the orthotopic model of RM-1 prostate cancer, and was superior to single GM-CSF- or TNFα-surface-modified vaccine. Moreover, the splenocytes from the GM-CSF/TNFα-vaccine-treated mice showed the most potent cytotoxicity on RM-1 cells and the highest production of RM-1-specific IFNγ. In addition, more CD4+and CD8+T cells infiltrated into the tumor sites in the GM-CSF/TNFα-vaccine-treated mice than in the GM-CSF- or TNFα-vaccine-treated mice. Therefore, our study demonstrated that the efficacy of RM-1 prostate cancer cell vaccine could be improved by conjugating both GM-CSF and TNFα simultaneously on the surface of cancer cells, and that this modification thus has a potential translational significance. |
| doi_str_mv | 10.1016/j.vaccine.2010.05.038 |
| format | Article |
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This study showed that the GM-CSF/TNFα-doubly surface-modified vaccine significantly extended the survival in the orthotopic model of RM-1 prostate cancer, and was superior to single GM-CSF- or TNFα-surface-modified vaccine. Moreover, the splenocytes from the GM-CSF/TNFα-vaccine-treated mice showed the most potent cytotoxicity on RM-1 cells and the highest production of RM-1-specific IFNγ. In addition, more CD4+and CD8+T cells infiltrated into the tumor sites in the GM-CSF/TNFα-vaccine-treated mice than in the GM-CSF- or TNFα-vaccine-treated mice. 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This study showed that the GM-CSF/TNFα-doubly surface-modified vaccine significantly extended the survival in the orthotopic model of RM-1 prostate cancer, and was superior to single GM-CSF- or TNFα-surface-modified vaccine. Moreover, the splenocytes from the GM-CSF/TNFα-vaccine-treated mice showed the most potent cytotoxicity on RM-1 cells and the highest production of RM-1-specific IFNγ. In addition, more CD4+and CD8+T cells infiltrated into the tumor sites in the GM-CSF/TNFα-vaccine-treated mice than in the GM-CSF- or TNFα-vaccine-treated mice. Therefore, our study demonstrated that the efficacy of RM-1 prostate cancer cell vaccine could be improved by conjugating both GM-CSF and TNFα simultaneously on the surface of cancer cells, and that this modification thus has a potential translational significance.</abstract><cop>Kidlington</cop><pub>Elsevier Limited</pub><doi>10.1016/j.vaccine.2010.05.038</doi></addata></record> |
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| ispartof | Vaccine, 2010-07, Vol.28 (31), p.4937 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Biological activity Bone marrow Cytokines Cytotoxicity Ethanol Lymphocytes Melanoma Prostate cancer Proteins Vaccines |
| title | A novel therapeutic vaccine of GM-CSF/TNF[alpha] surface-modified RM-1 cells against the orthotopic prostatic cancer |
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