Accelerated Evolution of Fetuin Family Proteins in Protobothrops flavoviridis (Habu Snake) Serum and the Discovery of an L1-Like Genomic Element in the Intronic Sequence of a Fetuin-Encoding Gene
Habu serum factor (HSF) and HSF-like protein (HLP) are fetuin family proteins isolated from Protobothrops flavoviridis (habu snake) serum with different physiological activities. A comparison of their cDNAs and intronic sequences revealed that nucleotide substitutions were primarily in protein-codin...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2013, Vol.77 (3), p.582-590 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | TANAKA, Yasuyoshi OYAMA, Sachiko HORI, Shin-ichi USHIO, Koya SHIOI, Narumi TERADA, Shigeyuki DESHIMARU, Masanobu |
| description | Habu serum factor (HSF) and HSF-like protein (HLP) are fetuin family proteins isolated from Protobothrops flavoviridis (habu snake) serum with different physiological activities. A comparison of their cDNAs and intronic sequences revealed that nucleotide substitutions were primarily in protein-coding regions, and the substitution patterns indicated accelerated evolution of these proteins. Genomic DNA fragment analysis, including intron 1, revealed a 6.6-kb insertion homologous to the full-length mammalian LINE1 (L1) retrotransposable element (PfL1) only in the HLP gene. This segment retains an open reading frame (ORF) that encodes a reverse transcriptase (RT)-like protein (PfRT). We further found that a large number of homologous segments have dispersed in the habu snake genome, although we could not determine the enzymatic activities of their products. Moreover, an analysis of habu snake liver RNA indicated active transcription of the PfRT genes, suggesting that high levels of RT activity in this snake have driven the evolution of unique phenotypes of venom enzymes and serum inhibitors of them. |
| doi_str_mv | 10.1271/bbb.120829 |
| format | Article |
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A comparison of their cDNAs and intronic sequences revealed that nucleotide substitutions were primarily in protein-coding regions, and the substitution patterns indicated accelerated evolution of these proteins. Genomic DNA fragment analysis, including intron 1, revealed a 6.6-kb insertion homologous to the full-length mammalian LINE1 (L1) retrotransposable element (PfL1) only in the HLP gene. This segment retains an open reading frame (ORF) that encodes a reverse transcriptase (RT)-like protein (PfRT). We further found that a large number of homologous segments have dispersed in the habu snake genome, although we could not determine the enzymatic activities of their products. Moreover, an analysis of habu snake liver RNA indicated active transcription of the PfRT genes, suggesting that high levels of RT activity in this snake have driven the evolution of unique phenotypes of venom enzymes and serum inhibitors of them.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.120829</identifier><identifier>PMID: 23470764</identifier><language>eng</language><publisher>England: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; Base Sequence ; Cloning, Molecular ; DNA, Complementary - genetics ; evolution ; Evolution, Molecular ; fetuin family proteins ; Fetuins - genetics ; Genetic Variation ; Genomics ; Introns - genetics ; Molecular Sequence Data ; protease inhibitor ; Retroelements - genetics ; retrotransposon ; RNA-Directed DNA Polymerase - genetics ; Transcription, Genetic ; Trimeresurus - blood ; venomous snake serum</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (3), p.582-590</ispartof><rights>2013 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2013</rights><rights>Copyright Japan Science and Technology Agency 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c588t-b11b84f67b1fb55254ff37de01b9330e8e1ec966b87b198feb94d4bfdd53f1a63</citedby><cites>FETCH-LOGICAL-c588t-b11b84f67b1fb55254ff37de01b9330e8e1ec966b87b198feb94d4bfdd53f1a63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23470764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TANAKA, Yasuyoshi</creatorcontrib><creatorcontrib>OYAMA, Sachiko</creatorcontrib><creatorcontrib>HORI, Shin-ichi</creatorcontrib><creatorcontrib>USHIO, Koya</creatorcontrib><creatorcontrib>SHIOI, Narumi</creatorcontrib><creatorcontrib>TERADA, Shigeyuki</creatorcontrib><creatorcontrib>DESHIMARU, Masanobu</creatorcontrib><title>Accelerated Evolution of Fetuin Family Proteins in Protobothrops flavoviridis (Habu Snake) Serum and the Discovery of an L1-Like Genomic Element in the Intronic Sequence of a Fetuin-Encoding Gene</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Habu serum factor (HSF) and HSF-like protein (HLP) are fetuin family proteins isolated from Protobothrops flavoviridis (habu snake) serum with different physiological activities. A comparison of their cDNAs and intronic sequences revealed that nucleotide substitutions were primarily in protein-coding regions, and the substitution patterns indicated accelerated evolution of these proteins. Genomic DNA fragment analysis, including intron 1, revealed a 6.6-kb insertion homologous to the full-length mammalian LINE1 (L1) retrotransposable element (PfL1) only in the HLP gene. This segment retains an open reading frame (ORF) that encodes a reverse transcriptase (RT)-like protein (PfRT). We further found that a large number of homologous segments have dispersed in the habu snake genome, although we could not determine the enzymatic activities of their products. Moreover, an analysis of habu snake liver RNA indicated active transcription of the PfRT genes, suggesting that high levels of RT activity in this snake have driven the evolution of unique phenotypes of venom enzymes and serum inhibitors of them.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - genetics</subject><subject>evolution</subject><subject>Evolution, Molecular</subject><subject>fetuin family proteins</subject><subject>Fetuins - genetics</subject><subject>Genetic Variation</subject><subject>Genomics</subject><subject>Introns - genetics</subject><subject>Molecular Sequence Data</subject><subject>protease inhibitor</subject><subject>Retroelements - genetics</subject><subject>retrotransposon</subject><subject>RNA-Directed DNA Polymerase - genetics</subject><subject>Transcription, Genetic</subject><subject>Trimeresurus - blood</subject><subject>venomous snake serum</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxiMEokvhwgMgS1xapBRP4vw7VmW3rbRSkRbOlu2MqdvEXmxn0T5fXwyHXTggJE4effrN5xn7y7K3QC-gaOCjlDIVtC26Z9kCStbkdcea59mCdlDnLavgJHsVwgOlSajgZXZSJIg2NVtkT5dK4YBeROzJcueGKRpnidNkhXEylqzEaIY9-exdRGMDSdJcO-nivXfbQPQgdm5nvOlNIGc3Qk5kY8UjnpMN-mkkwvYk3iP5ZIJyO_T72VxYsoZ8bR6RXKN1o1FkOeCINs4XzPitjd7ZpG_w-4RW4a-241T50irXG_tt7sbX2QsthoBvjudp9nW1_HJ1k6_vrm-vLte5qto25hJAtkzXjQQtq6qomNZl0yMF2ZUlxRYBVVfXsk1E12qUHeuZ1H1flRpEXZ5mZwffrXdpphD5mHbCYRAW3RQ41OlVgdKK_R8toauhYQUk9P1f6IObvE2LcGBNSwvK2pn6cKCUdyF41HzrzSj8ngPlcwp4SgE_pCDB746Wkxyx_4P-_vYEVAfAWO38KH44P_Q8iv3gvPbCKhN4-Q_jnzG1wJU</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>TANAKA, Yasuyoshi</creator><creator>OYAMA, Sachiko</creator><creator>HORI, Shin-ichi</creator><creator>USHIO, Koya</creator><creator>SHIOI, Narumi</creator><creator>TERADA, Shigeyuki</creator><creator>DESHIMARU, Masanobu</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2013</creationdate><title>Accelerated Evolution of Fetuin Family Proteins in Protobothrops flavoviridis (Habu Snake) Serum and the Discovery of an L1-Like Genomic Element in the Intronic Sequence of a Fetuin-Encoding Gene</title><author>TANAKA, Yasuyoshi ; OYAMA, Sachiko ; HORI, Shin-ichi ; USHIO, Koya ; SHIOI, Narumi ; TERADA, Shigeyuki ; DESHIMARU, Masanobu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c588t-b11b84f67b1fb55254ff37de01b9330e8e1ec966b87b198feb94d4bfdd53f1a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - genetics</topic><topic>evolution</topic><topic>Evolution, Molecular</topic><topic>fetuin family proteins</topic><topic>Fetuins - genetics</topic><topic>Genetic Variation</topic><topic>Genomics</topic><topic>Introns - genetics</topic><topic>Molecular Sequence Data</topic><topic>protease inhibitor</topic><topic>Retroelements - genetics</topic><topic>retrotransposon</topic><topic>RNA-Directed DNA Polymerase - genetics</topic><topic>Transcription, Genetic</topic><topic>Trimeresurus - blood</topic><topic>venomous snake serum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TANAKA, Yasuyoshi</creatorcontrib><creatorcontrib>OYAMA, Sachiko</creatorcontrib><creatorcontrib>HORI, Shin-ichi</creatorcontrib><creatorcontrib>USHIO, Koya</creatorcontrib><creatorcontrib>SHIOI, Narumi</creatorcontrib><creatorcontrib>TERADA, Shigeyuki</creatorcontrib><creatorcontrib>DESHIMARU, Masanobu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TANAKA, Yasuyoshi</au><au>OYAMA, Sachiko</au><au>HORI, Shin-ichi</au><au>USHIO, Koya</au><au>SHIOI, Narumi</au><au>TERADA, Shigeyuki</au><au>DESHIMARU, Masanobu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accelerated Evolution of Fetuin Family Proteins in Protobothrops flavoviridis (Habu Snake) Serum and the Discovery of an L1-Like Genomic Element in the Intronic Sequence of a Fetuin-Encoding Gene</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2013</date><risdate>2013</risdate><volume>77</volume><issue>3</issue><spage>582</spage><epage>590</epage><pages>582-590</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Habu serum factor (HSF) and HSF-like protein (HLP) are fetuin family proteins isolated from Protobothrops flavoviridis (habu snake) serum with different physiological activities. A comparison of their cDNAs and intronic sequences revealed that nucleotide substitutions were primarily in protein-coding regions, and the substitution patterns indicated accelerated evolution of these proteins. Genomic DNA fragment analysis, including intron 1, revealed a 6.6-kb insertion homologous to the full-length mammalian LINE1 (L1) retrotransposable element (PfL1) only in the HLP gene. This segment retains an open reading frame (ORF) that encodes a reverse transcriptase (RT)-like protein (PfRT). We further found that a large number of homologous segments have dispersed in the habu snake genome, although we could not determine the enzymatic activities of their products. Moreover, an analysis of habu snake liver RNA indicated active transcription of the PfRT genes, suggesting that high levels of RT activity in this snake have driven the evolution of unique phenotypes of venom enzymes and serum inhibitors of them.</abstract><cop>England</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>23470764</pmid><doi>10.1271/bbb.120829</doi><tpages>9</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2013, Vol.77 (3), p.582-590 |
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| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; Free Full-Text Journals in Chemistry |
| subjects | Animals Base Sequence Cloning, Molecular DNA, Complementary - genetics evolution Evolution, Molecular fetuin family proteins Fetuins - genetics Genetic Variation Genomics Introns - genetics Molecular Sequence Data protease inhibitor Retroelements - genetics retrotransposon RNA-Directed DNA Polymerase - genetics Transcription, Genetic Trimeresurus - blood venomous snake serum |
| title | Accelerated Evolution of Fetuin Family Proteins in Protobothrops flavoviridis (Habu Snake) Serum and the Discovery of an L1-Like Genomic Element in the Intronic Sequence of a Fetuin-Encoding Gene |
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