Using the Tyrosinase-Based Biosensor To Determine the Concentration of Phenolics in Wine
A tyrosinase-linked biosensor project described previously in this Journal has been expanded for upper-division undergraduate instrumentation courses; students use the biosensor that they fabricate to measure the concentration of phenolics in wine. The original project involved the use of a calibrat...
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Veröffentlicht in: | Journal of chemical education 2013-12, Vol.90 (12), p.1710-1712 |
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description | A tyrosinase-linked biosensor project described previously in this Journal has been expanded for upper-division undergraduate instrumentation courses; students use the biosensor that they fabricate to measure the concentration of phenolics in wine. The original project involved the use of a calibration curve and exposed students to the instruments and principles of electrochemical measurements. This extension, performed in three, 3-h laboratory periods, addresses three further topics of discussion: matrix effects in chemical analysis, the biochemical significance of antioxidants, and the use of statistical analysis techniques, such as the Student’s t test and ANOVA. |
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The original project involved the use of a calibration curve and exposed students to the instruments and principles of electrochemical measurements. This extension, performed in three, 3-h laboratory periods, addresses three further topics of discussion: matrix effects in chemical analysis, the biochemical significance of antioxidants, and the use of statistical analysis techniques, such as the Student’s t test and ANOVA.</description><identifier>ISSN: 0021-9584</identifier><identifier>EISSN: 1938-1328</identifier><identifier>DOI: 10.1021/ed300495a</identifier><identifier>CODEN: JCEDA8</identifier><language>eng</language><publisher>Easton: American Chemical Society and Division of Chemical Education, Inc</publisher><subject>Antioxidants ; Biochemistry ; Biosensors ; Calibration ; Chemical analysis ; College students ; Electrochemistry ; Instrumentation ; Organic chemistry ; Polyphenols ; Statistical analysis ; Students ; Tyrosinase ; Variance analysis ; Wines</subject><ispartof>Journal of chemical education, 2013-12, Vol.90 (12), p.1710-1712</ispartof><rights>Copyright © 2013 American Chemical Society and Division of Chemical Education, Inc.</rights><rights>Copyright American Chemical Society Dec 10, 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a287t-3124ec337a0d5965c2caf427c342dc55684a3322ebc9fab1a40800338903ff7c3</citedby><cites>FETCH-LOGICAL-a287t-3124ec337a0d5965c2caf427c342dc55684a3322ebc9fab1a40800338903ff7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ed300495a$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ed300495a$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids></links><search><creatorcontrib>Goodney, David E</creatorcontrib><creatorcontrib>Silverstein, Todd P</creatorcontrib><title>Using the Tyrosinase-Based Biosensor To Determine the Concentration of Phenolics in Wine</title><title>Journal of chemical education</title><addtitle>J. Chem. Educ</addtitle><description>A tyrosinase-linked biosensor project described previously in this Journal has been expanded for upper-division undergraduate instrumentation courses; students use the biosensor that they fabricate to measure the concentration of phenolics in wine. The original project involved the use of a calibration curve and exposed students to the instruments and principles of electrochemical measurements. This extension, performed in three, 3-h laboratory periods, addresses three further topics of discussion: matrix effects in chemical analysis, the biochemical significance of antioxidants, and the use of statistical analysis techniques, such as the Student’s t test and ANOVA.</description><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biosensors</subject><subject>Calibration</subject><subject>Chemical analysis</subject><subject>College students</subject><subject>Electrochemistry</subject><subject>Instrumentation</subject><subject>Organic chemistry</subject><subject>Polyphenols</subject><subject>Statistical analysis</subject><subject>Students</subject><subject>Tyrosinase</subject><subject>Variance analysis</subject><subject>Wines</subject><issn>0021-9584</issn><issn>1938-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpl0EtLAzEQAOAgCtbqwX8QEA8eVievbnK0rS8o6KFFb0uaTWxKm9Rke-i_N1oRwcsMw3zMMIPQOYFrApTc2JYBcCX0AeoRxWRFGJWHqAelWSkh-TE6yXkJQKhQsofeZtmHd9wtLJ7uUiyFzrYaltDioY_ZhhwTnkY8tp1Nax_stx3FYGzoku58DDg6_LKwIa68ydgH_FrYKTpyepXt2U_uo9n93XT0WE2eH55Gt5NKU1l3FSOUW8NYraEVaiAMNdpxWhvGaWuEGEiuGaPUzo1yek40BwnAmFTAnCusjy72czcpfmxt7ppl3KZQVjaE1wCSg5BFXe2VKTfmZF2zSX6t064h0Hw9rvl9XLGXe6tN_jPsn_sERENrNA</recordid><startdate>20131210</startdate><enddate>20131210</enddate><creator>Goodney, David E</creator><creator>Silverstein, Todd P</creator><general>American Chemical Society and Division of Chemical Education, Inc</general><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20131210</creationdate><title>Using the Tyrosinase-Based Biosensor To Determine the Concentration of Phenolics in Wine</title><author>Goodney, David E ; Silverstein, Todd P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a287t-3124ec337a0d5965c2caf427c342dc55684a3322ebc9fab1a40800338903ff7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biosensors</topic><topic>Calibration</topic><topic>Chemical analysis</topic><topic>College students</topic><topic>Electrochemistry</topic><topic>Instrumentation</topic><topic>Organic chemistry</topic><topic>Polyphenols</topic><topic>Statistical analysis</topic><topic>Students</topic><topic>Tyrosinase</topic><topic>Variance analysis</topic><topic>Wines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goodney, David E</creatorcontrib><creatorcontrib>Silverstein, Todd P</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Journal of chemical education</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goodney, David E</au><au>Silverstein, Todd P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using the Tyrosinase-Based Biosensor To Determine the Concentration of Phenolics in Wine</atitle><jtitle>Journal of chemical education</jtitle><addtitle>J. Chem. Educ</addtitle><date>2013-12-10</date><risdate>2013</risdate><volume>90</volume><issue>12</issue><spage>1710</spage><epage>1712</epage><pages>1710-1712</pages><issn>0021-9584</issn><eissn>1938-1328</eissn><coden>JCEDA8</coden><abstract>A tyrosinase-linked biosensor project described previously in this Journal has been expanded for upper-division undergraduate instrumentation courses; students use the biosensor that they fabricate to measure the concentration of phenolics in wine. The original project involved the use of a calibration curve and exposed students to the instruments and principles of electrochemical measurements. This extension, performed in three, 3-h laboratory periods, addresses three further topics of discussion: matrix effects in chemical analysis, the biochemical significance of antioxidants, and the use of statistical analysis techniques, such as the Student’s t test and ANOVA.</abstract><cop>Easton</cop><pub>American Chemical Society and Division of Chemical Education, Inc</pub><doi>10.1021/ed300495a</doi><tpages>3</tpages></addata></record> |
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subjects | Antioxidants Biochemistry Biosensors Calibration Chemical analysis College students Electrochemistry Instrumentation Organic chemistry Polyphenols Statistical analysis Students Tyrosinase Variance analysis Wines |
title | Using the Tyrosinase-Based Biosensor To Determine the Concentration of Phenolics in Wine |
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