Functional Overloading Facilitates the Regeneration of Injured Soleus Muscles in Mice
The effect of functional overloading on the regenerating process of injured skeletal muscle was investigated in 10-week-old male mice (C57BL/6J). Functional overloading on soleus of both hindlimbs was performed by cutting the distal tendons of plantaris and gastrocnemius muscles for 2 weeks before c...
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| Veröffentlicht in: | Journal of Physiological Sciences 2008, Vol.58(6), pp.397-404 |
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| creator | Morioka, Shigeta Goto, Katsumasa Kojima, Atsushi Naito, Toshihito Matsuba, Yusuke Akema, Tatsuo Fujiya, Hiroto Sugiura, Takao Ohira, Yoshinobu Beppu, Moroe Aoki, Haruhito Yoshioka, Toshitada |
| description | The effect of functional overloading on the regenerating process of injured skeletal muscle was investigated in 10-week-old male mice (C57BL/6J). Functional overloading on soleus of both hindlimbs was performed by cutting the distal tendons of plantaris and gastrocnemius muscles for 2 weeks before cardiotoxin (CTX) injection as the preconditioning and also during 10 weeks of recovery. To activate the necrosis- regeneration cycle, 0.1 ml of 10-μM CTX was injected into soleus muscle. The mean values of absolute muscle weight and the percentage of Pax7-positive nuclei in soleus were increased by the preconditioning. These values, as well as total muscle protein content, in the group with CTX injection plus overloading were larger than in the group with CTX injection alone. Fibers with central nucleus were noted in the group with CTX injection with or without overloading. The rate of disappearance of fibers having central nucleus during recovery was stimulated by overloading. Histological analyses revealed that the regeneration of injured soleus muscle with overloading proceeded more rapidly than the muscle without overloading. These results, in combination with previous lines of evidence, strongly suggest that functional overloading may facilitate the regeneration of injured skeletal muscles. |
| doi_str_mv | 10.2170/physiolsci.RP004008 |
| format | Article |
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Functional overloading on soleus of both hindlimbs was performed by cutting the distal tendons of plantaris and gastrocnemius muscles for 2 weeks before cardiotoxin (CTX) injection as the preconditioning and also during 10 weeks of recovery. To activate the necrosis- regeneration cycle, 0.1 ml of 10-μM CTX was injected into soleus muscle. The mean values of absolute muscle weight and the percentage of Pax7-positive nuclei in soleus were increased by the preconditioning. These values, as well as total muscle protein content, in the group with CTX injection plus overloading were larger than in the group with CTX injection alone. Fibers with central nucleus were noted in the group with CTX injection with or without overloading. The rate of disappearance of fibers having central nucleus during recovery was stimulated by overloading. Histological analyses revealed that the regeneration of injured soleus muscle with overloading proceeded more rapidly than the muscle without overloading. These results, in combination with previous lines of evidence, strongly suggest that functional overloading may facilitate the regeneration of injured skeletal muscles.</description><identifier>ISSN: 1880-6546</identifier><identifier>EISSN: 1880-6562</identifier><identifier>DOI: 10.2170/physiolsci.RP004008</identifier><identifier>PMID: 18845057</identifier><language>eng</language><publisher>Japan: PHYSIOLOGICAL SOCIETY OF JAPAN</publisher><subject>Animals ; Cell Differentiation ; Cell Nucleus - metabolism ; Cell Nucleus - pathology ; Cell Proliferation ; Disease Models, Animal ; loading ; Male ; Mice ; Mice, Inbred C57BL ; Muscle Contraction ; Muscle Fibers, Skeletal - metabolism ; Muscle Fibers, Skeletal - pathology ; Muscle Proteins - metabolism ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - pathology ; Muscle, Skeletal - physiopathology ; Muscular Diseases - chemically induced ; Muscular Diseases - pathology ; Muscular Diseases - physiopathology ; Necrosis ; Organ Size ; PAX7 Transcription Factor - metabolism ; Regeneration ; satellite cell ; skeletal muscle ; Stress, Mechanical ; Tendons - surgery ; Time Factors ; Toxins, Biological</subject><ispartof>The Journal of Physiological Sciences, 2008, Vol.58(6), pp.397-404</ispartof><rights>2008 by The Physiological Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c718t-7228e29fb90afa5c496736eb4c86c0856f2b5eeeedbc66446de750b92c5caac23</citedby><cites>FETCH-LOGICAL-c718t-7228e29fb90afa5c496736eb4c86c0856f2b5eeeedbc66446de750b92c5caac23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18845057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morioka, Shigeta</creatorcontrib><creatorcontrib>Goto, Katsumasa</creatorcontrib><creatorcontrib>Kojima, Atsushi</creatorcontrib><creatorcontrib>Naito, Toshihito</creatorcontrib><creatorcontrib>Matsuba, Yusuke</creatorcontrib><creatorcontrib>Akema, Tatsuo</creatorcontrib><creatorcontrib>Fujiya, Hiroto</creatorcontrib><creatorcontrib>Sugiura, Takao</creatorcontrib><creatorcontrib>Ohira, Yoshinobu</creatorcontrib><creatorcontrib>Beppu, Moroe</creatorcontrib><creatorcontrib>Aoki, Haruhito</creatorcontrib><creatorcontrib>Yoshioka, Toshitada</creatorcontrib><creatorcontrib>Yamaguchi University</creatorcontrib><creatorcontrib>Department of Orthopaedic Surgery</creatorcontrib><creatorcontrib>Toyohashi SOZO University</creatorcontrib><creatorcontrib>Faculty of Education</creatorcontrib><creatorcontrib>Department of Sports Medicine</creatorcontrib><creatorcontrib>Osaka University</creatorcontrib><creatorcontrib>Laboratory of Physiology</creatorcontrib><creatorcontrib>Graduate School of Medicine</creatorcontrib><creatorcontrib>Department of Physiology</creatorcontrib><creatorcontrib>St. Marianna University School of Medicine</creatorcontrib><creatorcontrib>Hirosaki Gakuin University</creatorcontrib><title>Functional Overloading Facilitates the Regeneration of Injured Soleus Muscles in Mice</title><title>Journal of Physiological Sciences</title><addtitle>J. Physiol. Sci</addtitle><description>The effect of functional overloading on the regenerating process of injured skeletal muscle was investigated in 10-week-old male mice (C57BL/6J). Functional overloading on soleus of both hindlimbs was performed by cutting the distal tendons of plantaris and gastrocnemius muscles for 2 weeks before cardiotoxin (CTX) injection as the preconditioning and also during 10 weeks of recovery. To activate the necrosis- regeneration cycle, 0.1 ml of 10-μM CTX was injected into soleus muscle. The mean values of absolute muscle weight and the percentage of Pax7-positive nuclei in soleus were increased by the preconditioning. These values, as well as total muscle protein content, in the group with CTX injection plus overloading were larger than in the group with CTX injection alone. Fibers with central nucleus were noted in the group with CTX injection with or without overloading. The rate of disappearance of fibers having central nucleus during recovery was stimulated by overloading. Histological analyses revealed that the regeneration of injured soleus muscle with overloading proceeded more rapidly than the muscle without overloading. These results, in combination with previous lines of evidence, strongly suggest that functional overloading may facilitate the regeneration of injured skeletal muscles.</description><subject>Animals</subject><subject>Cell Differentiation</subject><subject>Cell Nucleus - metabolism</subject><subject>Cell Nucleus - pathology</subject><subject>Cell Proliferation</subject><subject>Disease Models, Animal</subject><subject>loading</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Muscle Contraction</subject><subject>Muscle Fibers, Skeletal - metabolism</subject><subject>Muscle Fibers, Skeletal - pathology</subject><subject>Muscle Proteins - metabolism</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Muscular Diseases - chemically induced</subject><subject>Muscular Diseases - pathology</subject><subject>Muscular Diseases - physiopathology</subject><subject>Necrosis</subject><subject>Organ Size</subject><subject>PAX7 Transcription Factor - metabolism</subject><subject>Regeneration</subject><subject>satellite cell</subject><subject>skeletal muscle</subject><subject>Stress, Mechanical</subject><subject>Tendons - surgery</subject><subject>Time Factors</subject><subject>Toxins, Biological</subject><issn>1880-6546</issn><issn>1880-6562</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkN1q3DAQhU1oSdI0TxAogl5vKsv682UJ3TaQkJK210Iej9daFGkr2YG8fWV2k60uNIL55pzRqaqrml6zWtEvu_Elu-gzuOvHn5RySvVJdV5rTVdSSPbu7c3lWfUh521hZMv0aXVWGlxQoc6rP-s5wORisJ48PGPy0fYubMjagvNushNmMo1IHnGDAZNdUBIHchu2c8Ke_Ioe50zu5wy-oC6Qewf4sXo_WJ_x8lAvis-33zc_VncP329vvt6tQNV6WinGNLJ26FpqByuAt1I1EjsOWgLVQg6sE1hO34GUnMselaBdy0CAtcCai-rzXneX4t8Z82S2cU7lL9nUXGrdSs5koZo9BSnmnHAwu-SebHoxNTVLlOYYpXmNskx9OmjP3RP2x5lDdgVY74HSdWB9DN4FPG4AI9uNiyQrcoZSoalciqFNq0wx4a3iSvKj0DZPdoNvTjZNrqT6_3ZCG7m_Fo1XAEabDIbmH5Gco-0</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Morioka, Shigeta</creator><creator>Goto, Katsumasa</creator><creator>Kojima, Atsushi</creator><creator>Naito, Toshihito</creator><creator>Matsuba, Yusuke</creator><creator>Akema, Tatsuo</creator><creator>Fujiya, Hiroto</creator><creator>Sugiura, Takao</creator><creator>Ohira, Yoshinobu</creator><creator>Beppu, Moroe</creator><creator>Aoki, Haruhito</creator><creator>Yoshioka, Toshitada</creator><general>PHYSIOLOGICAL SOCIETY OF JAPAN</general><general>The Physiological Society of Japan</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope></search><sort><creationdate>20081201</creationdate><title>Functional Overloading Facilitates the Regeneration of Injured Soleus Muscles in Mice</title><author>Morioka, Shigeta ; Goto, Katsumasa ; Kojima, Atsushi ; Naito, Toshihito ; Matsuba, Yusuke ; Akema, Tatsuo ; Fujiya, Hiroto ; Sugiura, Takao ; Ohira, Yoshinobu ; Beppu, Moroe ; Aoki, Haruhito ; Yoshioka, Toshitada</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c718t-7228e29fb90afa5c496736eb4c86c0856f2b5eeeedbc66446de750b92c5caac23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Cell Differentiation</topic><topic>Cell Nucleus - metabolism</topic><topic>Cell Nucleus - pathology</topic><topic>Cell Proliferation</topic><topic>Disease Models, Animal</topic><topic>loading</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Muscle Contraction</topic><topic>Muscle Fibers, Skeletal - metabolism</topic><topic>Muscle Fibers, Skeletal - pathology</topic><topic>Muscle Proteins - metabolism</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscle, Skeletal - pathology</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Muscular Diseases - chemically induced</topic><topic>Muscular Diseases - pathology</topic><topic>Muscular Diseases - physiopathology</topic><topic>Necrosis</topic><topic>Organ Size</topic><topic>PAX7 Transcription Factor - metabolism</topic><topic>Regeneration</topic><topic>satellite cell</topic><topic>skeletal muscle</topic><topic>Stress, Mechanical</topic><topic>Tendons - surgery</topic><topic>Time Factors</topic><topic>Toxins, Biological</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morioka, Shigeta</creatorcontrib><creatorcontrib>Goto, Katsumasa</creatorcontrib><creatorcontrib>Kojima, Atsushi</creatorcontrib><creatorcontrib>Naito, Toshihito</creatorcontrib><creatorcontrib>Matsuba, Yusuke</creatorcontrib><creatorcontrib>Akema, Tatsuo</creatorcontrib><creatorcontrib>Fujiya, Hiroto</creatorcontrib><creatorcontrib>Sugiura, Takao</creatorcontrib><creatorcontrib>Ohira, Yoshinobu</creatorcontrib><creatorcontrib>Beppu, Moroe</creatorcontrib><creatorcontrib>Aoki, Haruhito</creatorcontrib><creatorcontrib>Yoshioka, Toshitada</creatorcontrib><creatorcontrib>Yamaguchi University</creatorcontrib><creatorcontrib>Department of Orthopaedic Surgery</creatorcontrib><creatorcontrib>Toyohashi SOZO University</creatorcontrib><creatorcontrib>Faculty of Education</creatorcontrib><creatorcontrib>Department of Sports Medicine</creatorcontrib><creatorcontrib>Osaka University</creatorcontrib><creatorcontrib>Laboratory of Physiology</creatorcontrib><creatorcontrib>Graduate School of Medicine</creatorcontrib><creatorcontrib>Department of Physiology</creatorcontrib><creatorcontrib>St. Marianna University School of Medicine</creatorcontrib><creatorcontrib>Hirosaki Gakuin University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Journal of Physiological Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morioka, Shigeta</au><au>Goto, Katsumasa</au><au>Kojima, Atsushi</au><au>Naito, Toshihito</au><au>Matsuba, Yusuke</au><au>Akema, Tatsuo</au><au>Fujiya, Hiroto</au><au>Sugiura, Takao</au><au>Ohira, Yoshinobu</au><au>Beppu, Moroe</au><au>Aoki, Haruhito</au><au>Yoshioka, Toshitada</au><aucorp>Yamaguchi University</aucorp><aucorp>Department of Orthopaedic Surgery</aucorp><aucorp>Toyohashi SOZO University</aucorp><aucorp>Faculty of Education</aucorp><aucorp>Department of Sports Medicine</aucorp><aucorp>Osaka University</aucorp><aucorp>Laboratory of Physiology</aucorp><aucorp>Graduate School of Medicine</aucorp><aucorp>Department of Physiology</aucorp><aucorp>St. Marianna University School of Medicine</aucorp><aucorp>Hirosaki Gakuin University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional Overloading Facilitates the Regeneration of Injured Soleus Muscles in Mice</atitle><jtitle>Journal of Physiological Sciences</jtitle><addtitle>J. Physiol. Sci</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>58</volume><issue>6</issue><spage>397</spage><epage>404</epage><pages>397-404</pages><issn>1880-6546</issn><eissn>1880-6562</eissn><abstract>The effect of functional overloading on the regenerating process of injured skeletal muscle was investigated in 10-week-old male mice (C57BL/6J). Functional overloading on soleus of both hindlimbs was performed by cutting the distal tendons of plantaris and gastrocnemius muscles for 2 weeks before cardiotoxin (CTX) injection as the preconditioning and also during 10 weeks of recovery. To activate the necrosis- regeneration cycle, 0.1 ml of 10-μM CTX was injected into soleus muscle. The mean values of absolute muscle weight and the percentage of Pax7-positive nuclei in soleus were increased by the preconditioning. These values, as well as total muscle protein content, in the group with CTX injection plus overloading were larger than in the group with CTX injection alone. Fibers with central nucleus were noted in the group with CTX injection with or without overloading. The rate of disappearance of fibers having central nucleus during recovery was stimulated by overloading. Histological analyses revealed that the regeneration of injured soleus muscle with overloading proceeded more rapidly than the muscle without overloading. These results, in combination with previous lines of evidence, strongly suggest that functional overloading may facilitate the regeneration of injured skeletal muscles.</abstract><cop>Japan</cop><pub>PHYSIOLOGICAL SOCIETY OF JAPAN</pub><pmid>18845057</pmid><doi>10.2170/physiolsci.RP004008</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of Physiological Sciences, 2008, Vol.58(6), pp.397-404 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Animals Cell Differentiation Cell Nucleus - metabolism Cell Nucleus - pathology Cell Proliferation Disease Models, Animal loading Male Mice Mice, Inbred C57BL Muscle Contraction Muscle Fibers, Skeletal - metabolism Muscle Fibers, Skeletal - pathology Muscle Proteins - metabolism Muscle, Skeletal - metabolism Muscle, Skeletal - pathology Muscle, Skeletal - physiopathology Muscular Diseases - chemically induced Muscular Diseases - pathology Muscular Diseases - physiopathology Necrosis Organ Size PAX7 Transcription Factor - metabolism Regeneration satellite cell skeletal muscle Stress, Mechanical Tendons - surgery Time Factors Toxins, Biological |
| title | Functional Overloading Facilitates the Regeneration of Injured Soleus Muscles in Mice |
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