RELATIONSHIP BETWEEN CAFFEINE-INDUCED OCULAR HYPERTENSION AND ULTRASTRUCTURE CHANGES OF NON-PIGMENTED CILIARY EPITHELIAL CELLS IN RATS
The purpose of this study was to morphologically assess a possible mechanism for caffeine-induced ocular hypertension. Taking into consideration the relationship between the secretion of aqueous humor and the ultrastructure of the ciliary body, the time course of the morphological features in the ci...
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| Veröffentlicht in: | Journal of toxicological sciences 1997/12/15, Vol.22(5), pp.447-454 |
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| creator | Kurata, Kazuyuki Maeda, Masayasu Nishida, Eiji Tsukuda, Ryoichi Suzuki, Tsuyoshi Ando, Takao Tokuriki, Mikihiko |
| description | The purpose of this study was to morphologically assess a possible mechanism for caffeine-induced ocular hypertension. Taking into consideration the relationship between the secretion of aqueous humor and the ultrastructure of the ciliary body, the time course of the morphological features in the ciliary epithelium when caffeine was administered intravenously to male Wistar rats was investigated by electron-microscopy. These morphological findings were also compared with the changes in the intraocular pressure (IOP). A significant increase in IOP was noted 15 min and 1 hr after a single dosing of caffeine alone. This change disappeared in all animals within 2 hr after dosing. The IOP in the animals receiving caffeine and the β-blocker befunolol, which lowers the IOP by inhibiting aqueous humor secretion, decreased significantly from 15 min after dosing, and this change persisted 2 hr after dosing. In electron-microscopy 15 min and/or 1 hr after dosing with caffeine, a slight dilatation in the lateral intercellular spaces near the basement membrane of the non-pigmented ciliary epithelium was observed and the interdigitations between the non-pigmented epithelial cells were intact. Reversal of these changes was observed 2 hr after dosing. On the other hand, the lateral intercellular spaces between the non-pigmented epithelial cells were markedly dilated and the interdigitations were disorganized following dosing with caffeine alone and in combination with befunolol. These results described here indicate that the intravenous administration of caffeine causes ocular hypertension and also changes in the non-pigmented ciliary epithelium, suggesting an enhancement of aqueous humor transportation. This paradigm in the rat is considered to be useful to further assess caffeine-induced ocular hypertension and for use as an animal model in glaucoma research associated with an aqueous humor secretion. |
| doi_str_mv | 10.2131/jts.22.5_447 |
| format | Article |
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Taking into consideration the relationship between the secretion of aqueous humor and the ultrastructure of the ciliary body, the time course of the morphological features in the ciliary epithelium when caffeine was administered intravenously to male Wistar rats was investigated by electron-microscopy. These morphological findings were also compared with the changes in the intraocular pressure (IOP). A significant increase in IOP was noted 15 min and 1 hr after a single dosing of caffeine alone. This change disappeared in all animals within 2 hr after dosing. The IOP in the animals receiving caffeine and the β-blocker befunolol, which lowers the IOP by inhibiting aqueous humor secretion, decreased significantly from 15 min after dosing, and this change persisted 2 hr after dosing. In electron-microscopy 15 min and/or 1 hr after dosing with caffeine, a slight dilatation in the lateral intercellular spaces near the basement membrane of the non-pigmented ciliary epithelium was observed and the interdigitations between the non-pigmented epithelial cells were intact. Reversal of these changes was observed 2 hr after dosing. On the other hand, the lateral intercellular spaces between the non-pigmented epithelial cells were markedly dilated and the interdigitations were disorganized following dosing with caffeine alone and in combination with befunolol. These results described here indicate that the intravenous administration of caffeine causes ocular hypertension and also changes in the non-pigmented ciliary epithelium, suggesting an enhancement of aqueous humor transportation. This paradigm in the rat is considered to be useful to further assess caffeine-induced ocular hypertension and for use as an animal model in glaucoma research associated with an aqueous humor secretion.</description><identifier>ISSN: 0388-1350</identifier><identifier>EISSN: 1880-3989</identifier><identifier>DOI: 10.2131/jts.22.5_447</identifier><identifier>PMID: 9442454</identifier><identifier>CODEN: JTSCDR</identifier><language>eng</language><publisher>Tokyo: The Japanese Society of Toxicology</publisher><subject>Adrenergic beta-Antagonists - pharmacology ; Animals ; Aqueous Humor - metabolism ; Biological and medical sciences ; Caffeine - toxicity ; Ciliary Body - drug effects ; Ciliary Body - ultrastructure ; Drug toxicity and drugs side effects treatment ; Epithelial Cells - drug effects ; Epithelial Cells - ultrastructure ; Injections, Intravenous ; Intraocular Pressure - drug effects ; Male ; Medical sciences ; Ocular Hypertension - chemically induced ; Pharmacology. Drug treatments ; Propanolamines - pharmacology ; Rat ; Rats ; Rats, Wistar ; Toxicity: eye</subject><ispartof>The Journal of Toxicological Sciences, 1997/12/15, Vol.22(5), pp.447-454</ispartof><rights>The Japanese Society of Toxicology Headquarters</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1997</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5487-c75c1b76e2382237500b01b81ce69ca3a2039a02faa59b62869d3a3710b090e73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1877,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2194030$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9442454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kurata, Kazuyuki</creatorcontrib><creatorcontrib>Maeda, Masayasu</creatorcontrib><creatorcontrib>Nishida, Eiji</creatorcontrib><creatorcontrib>Tsukuda, Ryoichi</creatorcontrib><creatorcontrib>Suzuki, Tsuyoshi</creatorcontrib><creatorcontrib>Ando, Takao</creatorcontrib><creatorcontrib>Tokuriki, Mikihiko</creatorcontrib><title>RELATIONSHIP BETWEEN CAFFEINE-INDUCED OCULAR HYPERTENSION AND ULTRASTRUCTURE CHANGES OF NON-PIGMENTED CILIARY EPITHELIAL CELLS IN RATS</title><title>Journal of toxicological sciences</title><addtitle>J Toxicol Sci</addtitle><description>The purpose of this study was to morphologically assess a possible mechanism for caffeine-induced ocular hypertension. Taking into consideration the relationship between the secretion of aqueous humor and the ultrastructure of the ciliary body, the time course of the morphological features in the ciliary epithelium when caffeine was administered intravenously to male Wistar rats was investigated by electron-microscopy. These morphological findings were also compared with the changes in the intraocular pressure (IOP). A significant increase in IOP was noted 15 min and 1 hr after a single dosing of caffeine alone. This change disappeared in all animals within 2 hr after dosing. The IOP in the animals receiving caffeine and the β-blocker befunolol, which lowers the IOP by inhibiting aqueous humor secretion, decreased significantly from 15 min after dosing, and this change persisted 2 hr after dosing. In electron-microscopy 15 min and/or 1 hr after dosing with caffeine, a slight dilatation in the lateral intercellular spaces near the basement membrane of the non-pigmented ciliary epithelium was observed and the interdigitations between the non-pigmented epithelial cells were intact. Reversal of these changes was observed 2 hr after dosing. On the other hand, the lateral intercellular spaces between the non-pigmented epithelial cells were markedly dilated and the interdigitations were disorganized following dosing with caffeine alone and in combination with befunolol. These results described here indicate that the intravenous administration of caffeine causes ocular hypertension and also changes in the non-pigmented ciliary epithelium, suggesting an enhancement of aqueous humor transportation. This paradigm in the rat is considered to be useful to further assess caffeine-induced ocular hypertension and for use as an animal model in glaucoma research associated with an aqueous humor secretion.</description><subject>Adrenergic beta-Antagonists - pharmacology</subject><subject>Animals</subject><subject>Aqueous Humor - metabolism</subject><subject>Biological and medical sciences</subject><subject>Caffeine - toxicity</subject><subject>Ciliary Body - drug effects</subject><subject>Ciliary Body - ultrastructure</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Injections, Intravenous</subject><subject>Intraocular Pressure - drug effects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Ocular Hypertension - chemically induced</subject><subject>Pharmacology. Drug treatments</subject><subject>Propanolamines - pharmacology</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Toxicity: eye</subject><issn>0388-1350</issn><issn>1880-3989</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE2P0zAYhCMEWsrCjSuSJTiS4q_EzjG4bmMpOFXiCO3Jcr0ptOq2S9we-AP7u9dLS0_vK80zM9IkyUcEpxgR9G17DFOMp5mllL1KJohzmJKCF6-TCSScp4hk8G3yLoQthJjBjN4kNwWlmGZ0kjy1si6NanRXqSX4Ls1PKTUQ5XwulZap0rNeyBloRF-XLajulrI1UnfRAEo9A31t2rIzbS9M30ogqlIvZAeaOdCNTpdq8UNqE_1C1aps74BcKlPJ-NdAyLrugNKgLU33PnmzdrswfLjc26SfSyOqtG4WSpR16jPKWepZ5tGK5QMmHGPCMghXEK048kNeeEcchqRwEK-dy4pVjnle3BNHGIpYAQdGbpPP59zH8fDnNISj3R5O4z5WWkRzzhnFDEXq65ny4yGEcVjbx3Hz4Ma_FkH7srmNm1uM7b_NI_7pEnpaPQz3V_gyctS_XHQXvNutR7f3m3DFMCooJDBi4oxtw9H9Gq66G48bvxteOlHB8nPv__Kr6n-70Q578gykL5Uw</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>Kurata, Kazuyuki</creator><creator>Maeda, Masayasu</creator><creator>Nishida, Eiji</creator><creator>Tsukuda, Ryoichi</creator><creator>Suzuki, Tsuyoshi</creator><creator>Ando, Takao</creator><creator>Tokuriki, Mikihiko</creator><general>The Japanese Society of Toxicology</general><general>Japanese Society of Toxicology</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>1997</creationdate><title>RELATIONSHIP BETWEEN CAFFEINE-INDUCED OCULAR HYPERTENSION AND ULTRASTRUCTURE CHANGES OF NON-PIGMENTED CILIARY EPITHELIAL CELLS IN RATS</title><author>Kurata, Kazuyuki ; Maeda, Masayasu ; Nishida, Eiji ; Tsukuda, Ryoichi ; Suzuki, Tsuyoshi ; Ando, Takao ; Tokuriki, Mikihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5487-c75c1b76e2382237500b01b81ce69ca3a2039a02faa59b62869d3a3710b090e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Adrenergic beta-Antagonists - pharmacology</topic><topic>Animals</topic><topic>Aqueous Humor - metabolism</topic><topic>Biological and medical sciences</topic><topic>Caffeine - toxicity</topic><topic>Ciliary Body - drug effects</topic><topic>Ciliary Body - ultrastructure</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - ultrastructure</topic><topic>Injections, Intravenous</topic><topic>Intraocular Pressure - drug effects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Ocular Hypertension - chemically induced</topic><topic>Pharmacology. Drug treatments</topic><topic>Propanolamines - pharmacology</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Toxicity: eye</topic><toplevel>online_resources</toplevel><creatorcontrib>Kurata, Kazuyuki</creatorcontrib><creatorcontrib>Maeda, Masayasu</creatorcontrib><creatorcontrib>Nishida, Eiji</creatorcontrib><creatorcontrib>Tsukuda, Ryoichi</creatorcontrib><creatorcontrib>Suzuki, Tsuyoshi</creatorcontrib><creatorcontrib>Ando, Takao</creatorcontrib><creatorcontrib>Tokuriki, Mikihiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Journal of toxicological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kurata, Kazuyuki</au><au>Maeda, Masayasu</au><au>Nishida, Eiji</au><au>Tsukuda, Ryoichi</au><au>Suzuki, Tsuyoshi</au><au>Ando, Takao</au><au>Tokuriki, Mikihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RELATIONSHIP BETWEEN CAFFEINE-INDUCED OCULAR HYPERTENSION AND ULTRASTRUCTURE CHANGES OF NON-PIGMENTED CILIARY EPITHELIAL CELLS IN RATS</atitle><jtitle>Journal of toxicological sciences</jtitle><addtitle>J Toxicol Sci</addtitle><date>1997</date><risdate>1997</risdate><volume>22</volume><issue>5</issue><spage>447</spage><epage>454</epage><pages>447-454</pages><issn>0388-1350</issn><eissn>1880-3989</eissn><coden>JTSCDR</coden><abstract>The purpose of this study was to morphologically assess a possible mechanism for caffeine-induced ocular hypertension. Taking into consideration the relationship between the secretion of aqueous humor and the ultrastructure of the ciliary body, the time course of the morphological features in the ciliary epithelium when caffeine was administered intravenously to male Wistar rats was investigated by electron-microscopy. These morphological findings were also compared with the changes in the intraocular pressure (IOP). A significant increase in IOP was noted 15 min and 1 hr after a single dosing of caffeine alone. This change disappeared in all animals within 2 hr after dosing. The IOP in the animals receiving caffeine and the β-blocker befunolol, which lowers the IOP by inhibiting aqueous humor secretion, decreased significantly from 15 min after dosing, and this change persisted 2 hr after dosing. In electron-microscopy 15 min and/or 1 hr after dosing with caffeine, a slight dilatation in the lateral intercellular spaces near the basement membrane of the non-pigmented ciliary epithelium was observed and the interdigitations between the non-pigmented epithelial cells were intact. Reversal of these changes was observed 2 hr after dosing. On the other hand, the lateral intercellular spaces between the non-pigmented epithelial cells were markedly dilated and the interdigitations were disorganized following dosing with caffeine alone and in combination with befunolol. These results described here indicate that the intravenous administration of caffeine causes ocular hypertension and also changes in the non-pigmented ciliary epithelium, suggesting an enhancement of aqueous humor transportation. This paradigm in the rat is considered to be useful to further assess caffeine-induced ocular hypertension and for use as an animal model in glaucoma research associated with an aqueous humor secretion.</abstract><cop>Tokyo</cop><pub>The Japanese Society of Toxicology</pub><pmid>9442454</pmid><doi>10.2131/jts.22.5_447</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0388-1350 |
| ispartof | The Journal of Toxicological Sciences, 1997/12/15, Vol.22(5), pp.447-454 |
| issn | 0388-1350 1880-3989 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Adrenergic beta-Antagonists - pharmacology Animals Aqueous Humor - metabolism Biological and medical sciences Caffeine - toxicity Ciliary Body - drug effects Ciliary Body - ultrastructure Drug toxicity and drugs side effects treatment Epithelial Cells - drug effects Epithelial Cells - ultrastructure Injections, Intravenous Intraocular Pressure - drug effects Male Medical sciences Ocular Hypertension - chemically induced Pharmacology. Drug treatments Propanolamines - pharmacology Rat Rats Rats, Wistar Toxicity: eye |
| title | RELATIONSHIP BETWEEN CAFFEINE-INDUCED OCULAR HYPERTENSION AND ULTRASTRUCTURE CHANGES OF NON-PIGMENTED CILIARY EPITHELIAL CELLS IN RATS |
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