Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O

Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O

To develop an effective method to isolate an injured pathogenic Yersinia enterocolitica O:8 organism from environmental samples, we compared the isolation of freeze-injured and non-injured Y. enterocolitica O:8 and found that the isolation was more successful when immuno-magnetic separation (IMS) wi...

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Veröffentlicht in:Journal of veterinary medical science 2006-03, Vol.68 (3), p.195
Hauptverfasser: KOUJITANI, Eiko, HORISAKA, Tomoko, NOMURA, Yoshihiro, HARA-KUDO, Yukiko, Tomomitsu OKATANI, Alexandre, IWATA, Taketoshi, KUMAGAI, Susumu, HAYASHIDANI, Hideki
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container_issue 3
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container_title Journal of veterinary medical science
container_volume 68
creator KOUJITANI, Eiko
HORISAKA, Tomoko
NOMURA, Yoshihiro
HARA-KUDO, Yukiko
Tomomitsu OKATANI, Alexandre
IWATA, Taketoshi
KUMAGAI, Susumu
HAYASHIDANI, Hideki
description To develop an effective method to isolate an injured pathogenic Yersinia enterocolitica O:8 organism from environmental samples, we compared the isolation of freeze-injured and non-injured Y. enterocolitica O:8 and found that the isolation was more successful when immuno-magnetic separation (IMS) with anti-Y. enterocolitica O:8 antibody was used. Plating onto cefsulodin-irgasan-novobiocin (CIN) agar and Virulent Yersinia enterocolitica (VYE) agar by means of the agar layer method was found to be effective in isolating the injured cells. The alkali treatment which is generally used for selective detection of Yersinia organism failed to isolate freeze-injured pathogenic Y. enterocolitica O:8 cells. Recovery methods without using the alkali treatment were superior for detecting freeze-injured Y. enterocolitica O:8. Our results demonstrate that the IMS and the agar layer methods should be used to isolate injured pathogenic Yersinia organisms from environmental samples such as water.
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title Immuno-Magnetic Separation and Agar Layer Methods for the Isolation of Freeze-Injured Yersinia enterocolitica O
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