QTL analysis of net-like cracking in soybean [Glycine max] seed coats
There are two types of seed coat cracking that are controlled genetically in soybean: Type I with irregular cracks and Type II with net-like cracks. This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A Fsub(2) population and Fsub(3) lines derived from a cr...
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| Veröffentlicht in: | Breeding Science 2010, Vol.60(1), pp.28-33 |
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| creator | Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan) Githiri, S.M Benitez, E.R Takahashi, R |
| description | There are two types of seed coat cracking that are controlled genetically in soybean: Type I with irregular cracks and Type II with net-like cracks. This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A Fsub(2) population and Fsub(3) lines derived from a cross between Uzuramame with black seed coats having net-like cracking, and a Clark mutant line with black seed coats were used in this study. Degree of cracking in each seed was scored by cracking index (no cracking, 0 to severe cracking, 4) and was averaged per plant (average cracking index, ACI). One morphological and 189 SSR markers were analyzed and assigned to 31 linkage groups spanning 2463 cM. Two QTLs for ACI (ncr1 and ncr2) were identified in molecular linkage group C1 with a distance of about 62 cM. The ncr1 and ncr2 had LOD scores of 5.90 and 8.61, accounting for 16.0 and 32.4% of phenotypic variation, respectively. The alleles from Uzuramame at the QTLs conferred increased ACI. The effects of QTLs identified in the Fsub(2) population were confirmed in the Fsub(3) lines. No epistatic interaction was observed between the QTLs. |
| doi_str_mv | 10.1270/jsbbs.60.28 |
| format | Article |
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This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A Fsub(2) population and Fsub(3) lines derived from a cross between Uzuramame with black seed coats having net-like cracking, and a Clark mutant line with black seed coats were used in this study. Degree of cracking in each seed was scored by cracking index (no cracking, 0 to severe cracking, 4) and was averaged per plant (average cracking index, ACI). One morphological and 189 SSR markers were analyzed and assigned to 31 linkage groups spanning 2463 cM. Two QTLs for ACI (ncr1 and ncr2) were identified in molecular linkage group C1 with a distance of about 62 cM. The ncr1 and ncr2 had LOD scores of 5.90 and 8.61, accounting for 16.0 and 32.4% of phenotypic variation, respectively. The alleles from Uzuramame at the QTLs conferred increased ACI. The effects of QTLs identified in the Fsub(2) population were confirmed in the Fsub(3) lines. No epistatic interaction was observed between the QTLs.</description><identifier>ISSN: 1344-7610</identifier><identifier>EISSN: 1347-3735</identifier><identifier>DOI: 10.1270/jsbbs.60.28</identifier><language>eng</language><publisher>Tokyo: Japanese Society of Breeding</publisher><subject>GENETIC MARKERS ; GLYCINE MAX ; http://www.fao.org/aos/agrovoc#c_24030 ; http://www.fao.org/aos/agrovoc#c_3301 ; http://www.fao.org/aos/agrovoc#c_37974 ; http://www.fao.org/aos/agrovoc#c_49903 ; http://www.fao.org/aos/agrovoc#c_7681 ; LOCI DE RASGOS CUANTITATIVOS ; LOCUS DES CARACTERES QUANTITATIFS ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MORFOLOGIA ; MORPHOLOGIE ; MORPHOLOGY ; net-like cracking ; QTL analysis ; QUANTITATIVE TRAIT LOCI ; seed coat cracking ; soybean ; TESTA</subject><ispartof>Breeding Science, 2010, Vol.60(1), pp.28-33</ispartof><rights>2010 by JAPANESE SOCIETY OF BREEDING</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-535febd78c223a6e218b7679fce576fc6c0f1bab0ccaf01cdc8f9f80ee2e4d443</citedby><cites>FETCH-LOGICAL-c526t-535febd78c223a6e218b7679fce576fc6c0f1bab0ccaf01cdc8f9f80ee2e4d443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan)</creatorcontrib><creatorcontrib>Githiri, S.M</creatorcontrib><creatorcontrib>Benitez, E.R</creatorcontrib><creatorcontrib>Takahashi, R</creatorcontrib><title>QTL analysis of net-like cracking in soybean [Glycine max] seed coats</title><title>Breeding Science</title><addtitle>Breeding Science</addtitle><description>There are two types of seed coat cracking that are controlled genetically in soybean: Type I with irregular cracks and Type II with net-like cracks. This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A Fsub(2) population and Fsub(3) lines derived from a cross between Uzuramame with black seed coats having net-like cracking, and a Clark mutant line with black seed coats were used in this study. Degree of cracking in each seed was scored by cracking index (no cracking, 0 to severe cracking, 4) and was averaged per plant (average cracking index, ACI). One morphological and 189 SSR markers were analyzed and assigned to 31 linkage groups spanning 2463 cM. Two QTLs for ACI (ncr1 and ncr2) were identified in molecular linkage group C1 with a distance of about 62 cM. The ncr1 and ncr2 had LOD scores of 5.90 and 8.61, accounting for 16.0 and 32.4% of phenotypic variation, respectively. The alleles from Uzuramame at the QTLs conferred increased ACI. The effects of QTLs identified in the Fsub(2) population were confirmed in the Fsub(3) lines. No epistatic interaction was observed between the QTLs.</description><subject>GENETIC MARKERS</subject><subject>GLYCINE MAX</subject><subject>http://www.fao.org/aos/agrovoc#c_24030</subject><subject>http://www.fao.org/aos/agrovoc#c_3301</subject><subject>http://www.fao.org/aos/agrovoc#c_37974</subject><subject>http://www.fao.org/aos/agrovoc#c_49903</subject><subject>http://www.fao.org/aos/agrovoc#c_7681</subject><subject>LOCI DE RASGOS CUANTITATIVOS</subject><subject>LOCUS DES CARACTERES QUANTITATIFS</subject><subject>MARCADORES GENETICOS</subject><subject>MARQUEUR GENETIQUE</subject><subject>MORFOLOGIA</subject><subject>MORPHOLOGIE</subject><subject>MORPHOLOGY</subject><subject>net-like cracking</subject><subject>QTL analysis</subject><subject>QUANTITATIVE TRAIT LOCI</subject><subject>seed coat cracking</subject><subject>soybean</subject><subject>TESTA</subject><issn>1344-7610</issn><issn>1347-3735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNpFkF1LwzAUhoMoOKdXXgsBL6UzH23S3iljTmWgwrwSCWl6MtN17Uw6sP_eamXenHPgfXg5PAidUzKhTJLrMuR5mAgyYekBGlEey4hLnhz-3nEkBSXH6CSEkhCWkDgZodnLcoF1rasuuIAbi2too8qtARuvzdrVK-xqHJouB13jt3nVGVcD3uivdxwACmwa3YZTdGR1FeDsb4_R691sOb2PFk_zh-ntIjIJE22U8MRCXsjUMMa1AEbTXAqZWQOJFNYIQyzNdU6M0ZZQU5jUZjYlAAziIo75GF0OvVvffO4gtKpsdr7_PigaCyklzbjoqauBMr4JwYNVW-822neKEvXjSf16UoIolvb0zUCXodUr2LPat85U8M_SYbB0H5kP7RXUfcXFUGF1o_TKu6AenxmhpNdMM8a_AaKYfDU</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan)</creator><creator>Githiri, S.M</creator><creator>Benitez, E.R</creator><creator>Takahashi, R</creator><general>Japanese Society of Breeding</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>2010</creationdate><title>QTL analysis of net-like cracking in soybean [Glycine max] seed coats</title><author>Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan) ; Githiri, S.M ; Benitez, E.R ; Takahashi, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-535febd78c223a6e218b7679fce576fc6c0f1bab0ccaf01cdc8f9f80ee2e4d443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>GENETIC MARKERS</topic><topic>GLYCINE MAX</topic><topic>http://www.fao.org/aos/agrovoc#c_24030</topic><topic>http://www.fao.org/aos/agrovoc#c_3301</topic><topic>http://www.fao.org/aos/agrovoc#c_37974</topic><topic>http://www.fao.org/aos/agrovoc#c_49903</topic><topic>http://www.fao.org/aos/agrovoc#c_7681</topic><topic>LOCI DE RASGOS CUANTITATIVOS</topic><topic>LOCUS DES CARACTERES QUANTITATIFS</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>MORFOLOGIA</topic><topic>MORPHOLOGIE</topic><topic>MORPHOLOGY</topic><topic>net-like cracking</topic><topic>QTL analysis</topic><topic>QUANTITATIVE TRAIT LOCI</topic><topic>seed coat cracking</topic><topic>soybean</topic><topic>TESTA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan)</creatorcontrib><creatorcontrib>Githiri, S.M</creatorcontrib><creatorcontrib>Benitez, E.R</creatorcontrib><creatorcontrib>Takahashi, R</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Breeding Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyoo, M.E., Tsukuba Univ., Ibaraki (Japan)</au><au>Githiri, S.M</au><au>Benitez, E.R</au><au>Takahashi, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>QTL analysis of net-like cracking in soybean [Glycine max] seed coats</atitle><jtitle>Breeding Science</jtitle><addtitle>Breeding Science</addtitle><date>2010</date><risdate>2010</risdate><volume>60</volume><issue>1</issue><spage>28</spage><epage>33</epage><pages>28-33</pages><issn>1344-7610</issn><eissn>1347-3735</eissn><abstract>There are two types of seed coat cracking that are controlled genetically in soybean: Type I with irregular cracks and Type II with net-like cracks. This study was conducted to determine the genetic basis of Type II cracking using DNA markers. A Fsub(2) population and Fsub(3) lines derived from a cross between Uzuramame with black seed coats having net-like cracking, and a Clark mutant line with black seed coats were used in this study. Degree of cracking in each seed was scored by cracking index (no cracking, 0 to severe cracking, 4) and was averaged per plant (average cracking index, ACI). One morphological and 189 SSR markers were analyzed and assigned to 31 linkage groups spanning 2463 cM. Two QTLs for ACI (ncr1 and ncr2) were identified in molecular linkage group C1 with a distance of about 62 cM. The ncr1 and ncr2 had LOD scores of 5.90 and 8.61, accounting for 16.0 and 32.4% of phenotypic variation, respectively. The alleles from Uzuramame at the QTLs conferred increased ACI. The effects of QTLs identified in the Fsub(2) population were confirmed in the Fsub(3) lines. No epistatic interaction was observed between the QTLs.</abstract><cop>Tokyo</cop><pub>Japanese Society of Breeding</pub><doi>10.1270/jsbbs.60.28</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | GENETIC MARKERS GLYCINE MAX http://www.fao.org/aos/agrovoc#c_24030 http://www.fao.org/aos/agrovoc#c_3301 http://www.fao.org/aos/agrovoc#c_37974 http://www.fao.org/aos/agrovoc#c_49903 http://www.fao.org/aos/agrovoc#c_7681 LOCI DE RASGOS CUANTITATIVOS LOCUS DES CARACTERES QUANTITATIFS MARCADORES GENETICOS MARQUEUR GENETIQUE MORFOLOGIA MORPHOLOGIE MORPHOLOGY net-like cracking QTL analysis QUANTITATIVE TRAIT LOCI seed coat cracking soybean TESTA |
| title | QTL analysis of net-like cracking in soybean [Glycine max] seed coats |
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