A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea
We analyzed 124 markers for linkage in 138 F2 plants derived from a cross between 2 inbred lines, cabbage and kale (both Brassica oleracea). Ninety-nine RAPDs, 21 RFLPs, 2 isozyme genes, and 2 morphological characters were used to construct a genetic map spanning 9 linkage groups covering 823.6 cM o...
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| Veröffentlicht in: | Breeding Science 1999, Vol.49(4), pp.257-265 |
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| creator | Moriguchi, K. (Mikado Seed Growers Co. Ltd., Chiba (Japan)) Kimizuka Takagi, C Ishii, K Nomura, K |
| description | We analyzed 124 markers for linkage in 138 F2 plants derived from a cross between 2 inbred lines, cabbage and kale (both Brassica oleracea). Ninety-nine RAPDs, 21 RFLPs, 2 isozyme genes, and 2 morphological characters were used to construct a genetic map spanning 9 linkage groups covering 823.6 cM of the B. oleracea genome. The number of mapped loci for each linkage group ranged from 9 to 23. The average interval between 2 loci was 7.16 cM. The maximum distance between two loci in the linkage groups was 283 cM. Most of the markers segregated in a normal Mendelian ratio. However, biased segregation was observed at 10 loci. Most of the loci were clustered in a particular area of the genome, and showed an over-abundance of the male parent, indicating the presence of a lethal allele in the neighborhood of these markers or a quantitative selection advantage for one or more alleles. One QTL for resistance to clubroot disease was identified. QTL mapping confirmed the identification of the probable location and effect of a QTL on linkage group 3. The QTL was separated from the nearest marker by a distance of 1.0 cM, and its R2 value was 0.300 indicating phenotypic variation |
| doi_str_mv | 10.1270/jsbbs.49.257 |
| format | Article |
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(Mikado Seed Growers Co. Ltd., Chiba (Japan)) ; Kimizuka Takagi, C ; Ishii, K ; Nomura, K</creator><creatorcontrib>Moriguchi, K. (Mikado Seed Growers Co. Ltd., Chiba (Japan)) ; Kimizuka Takagi, C ; Ishii, K ; Nomura, K</creatorcontrib><description>We analyzed 124 markers for linkage in 138 F2 plants derived from a cross between 2 inbred lines, cabbage and kale (both Brassica oleracea). Ninety-nine RAPDs, 21 RFLPs, 2 isozyme genes, and 2 morphological characters were used to construct a genetic map spanning 9 linkage groups covering 823.6 cM of the B. oleracea genome. The number of mapped loci for each linkage group ranged from 9 to 23. The average interval between 2 loci was 7.16 cM. The maximum distance between two loci in the linkage groups was 283 cM. Most of the markers segregated in a normal Mendelian ratio. However, biased segregation was observed at 10 loci. Most of the loci were clustered in a particular area of the genome, and showed an over-abundance of the male parent, indicating the presence of a lethal allele in the neighborhood of these markers or a quantitative selection advantage for one or more alleles. One QTL for resistance to clubroot disease was identified. QTL mapping confirmed the identification of the probable location and effect of a QTL on linkage group 3. The QTL was separated from the nearest marker by a distance of 1.0 cM, and its R2 value was 0.300 indicating phenotypic variation</description><identifier>ISSN: 1344-7610</identifier><identifier>EISSN: 1347-3735</identifier><identifier>DOI: 10.1270/jsbbs.49.257</identifier><language>eng</language><publisher>Tokyo: Japanese Society of Breeding</publisher><subject>Agronomy. Soil science and plant productions ; ANALISIS MICROBIOLOGICO ; ANALYSE MICROBIOLOGIQUE ; Biological and medical sciences ; BRASSICA OLERACEA ; CARTE GENETIQUE ; DISEASE RESISTANCE ; Fundamental and applied biological sciences. Psychology ; GENETIC MAPS ; Genetics and breeding of economic plants ; ISOENZIMAS ; ISOENZYME ; ISOENZYMES ; MAPAS GENETICOS ; METHODE STATISTIQUE ; METODOS ESTADISTICOS ; MICROBIOLOGICAL ANALYSIS ; MORFOGENESIS ; MORPHOGENESE ; MORPHOGENESIS ; Pest resistance ; Plant pathogens ; PLASMODIOPHORA BRASSICAE ; RAPD ; RESISTANCE AUX MALADIES ; RESISTENCIA A LA ENFERMEDAD ; RFLP ; STATISTICAL METHODS ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Breeding Science, 1999, Vol.49(4), pp.257-265</ispartof><rights>Japanese Society of Breeding</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c560t-40d26f2ea0df98b2802ed1dd9505cb7c074defd8c13a7c5eb86e12f60a2b9d743</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1271683$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Moriguchi, K. (Mikado Seed Growers Co. Ltd., Chiba (Japan))</creatorcontrib><creatorcontrib>Kimizuka Takagi, C</creatorcontrib><creatorcontrib>Ishii, K</creatorcontrib><creatorcontrib>Nomura, K</creatorcontrib><title>A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea</title><title>Breeding Science</title><addtitle>Breeding Science</addtitle><description>We analyzed 124 markers for linkage in 138 F2 plants derived from a cross between 2 inbred lines, cabbage and kale (both Brassica oleracea). Ninety-nine RAPDs, 21 RFLPs, 2 isozyme genes, and 2 morphological characters were used to construct a genetic map spanning 9 linkage groups covering 823.6 cM of the B. oleracea genome. The number of mapped loci for each linkage group ranged from 9 to 23. The average interval between 2 loci was 7.16 cM. The maximum distance between two loci in the linkage groups was 283 cM. Most of the markers segregated in a normal Mendelian ratio. However, biased segregation was observed at 10 loci. Most of the loci were clustered in a particular area of the genome, and showed an over-abundance of the male parent, indicating the presence of a lethal allele in the neighborhood of these markers or a quantitative selection advantage for one or more alleles. One QTL for resistance to clubroot disease was identified. QTL mapping confirmed the identification of the probable location and effect of a QTL on linkage group 3. The QTL was separated from the nearest marker by a distance of 1.0 cM, and its R2 value was 0.300 indicating phenotypic variation</description><subject>Agronomy. Soil science and plant productions</subject><subject>ANALISIS MICROBIOLOGICO</subject><subject>ANALYSE MICROBIOLOGIQUE</subject><subject>Biological and medical sciences</subject><subject>BRASSICA OLERACEA</subject><subject>CARTE GENETIQUE</subject><subject>DISEASE RESISTANCE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC MAPS</subject><subject>Genetics and breeding of economic plants</subject><subject>ISOENZIMAS</subject><subject>ISOENZYME</subject><subject>ISOENZYMES</subject><subject>MAPAS GENETICOS</subject><subject>METHODE STATISTIQUE</subject><subject>METODOS ESTADISTICOS</subject><subject>MICROBIOLOGICAL ANALYSIS</subject><subject>MORFOGENESIS</subject><subject>MORPHOGENESE</subject><subject>MORPHOGENESIS</subject><subject>Pest resistance</subject><subject>Plant pathogens</subject><subject>PLASMODIOPHORA BRASSICAE</subject><subject>RAPD</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>RFLP</subject><subject>STATISTICAL METHODS</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><issn>1344-7610</issn><issn>1347-3735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpFkE1vEzEQhlcIJErpjSuSJThmg-31x-4xlJYWRWpalbM1a4_TDZt1am8O4cJfr9Otig8ea-adxzNvUXxidM64pt82qW3TXDRzLvWb4oRVQpeVruTb57cotWL0ffEhpQ2lXFIhT4p_C7LGAcfOki3sSAsJHQkDuVusfszI3eVyNSNdCn8PW5yRbYi7h9CHdWehz_r4B2MiMDhye7_MEfpD6hLxIRLb79sYwkgi5tQIg0XSDeR7hJRyNwk9RrAIH4t3HvqEZy_xtPh9eXF_flUub35eny-WpZWKjqWgjivPEajzTd3ymnJ0zLlGUmlbbakWDr2rLatAW4ltrZBxryjwtnFaVKfFl4m7i-Fxj2k0m7CPeeJkmFBaK6mYyqrZpLIxpBTRm13s8p4Hw6g5WmyeLTaiMdniLP_6AoWUHfExr9ml_z1cM1VXWXYxyTbZiDW-1iFm23ucmKxpmiNXTFfGv9btA0SDQ-Z8njgegoF1zF_9WnF6PBVjrHoCkAKeSw</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Moriguchi, K. (Mikado Seed Growers Co. 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Soil science and plant productions</topic><topic>ANALISIS MICROBIOLOGICO</topic><topic>ANALYSE MICROBIOLOGIQUE</topic><topic>Biological and medical sciences</topic><topic>BRASSICA OLERACEA</topic><topic>CARTE GENETIQUE</topic><topic>DISEASE RESISTANCE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC MAPS</topic><topic>Genetics and breeding of economic plants</topic><topic>ISOENZIMAS</topic><topic>ISOENZYME</topic><topic>ISOENZYMES</topic><topic>MAPAS GENETICOS</topic><topic>METHODE STATISTIQUE</topic><topic>METODOS ESTADISTICOS</topic><topic>MICROBIOLOGICAL ANALYSIS</topic><topic>MORFOGENESIS</topic><topic>MORPHOGENESE</topic><topic>MORPHOGENESIS</topic><topic>Pest resistance</topic><topic>Plant pathogens</topic><topic>PLASMODIOPHORA BRASSICAE</topic><topic>RAPD</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>RFLP</topic><topic>STATISTICAL METHODS</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moriguchi, K. (Mikado Seed Growers Co. Ltd., Chiba (Japan))</creatorcontrib><creatorcontrib>Kimizuka Takagi, C</creatorcontrib><creatorcontrib>Ishii, K</creatorcontrib><creatorcontrib>Nomura, K</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Breeding Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moriguchi, K. (Mikado Seed Growers Co. Ltd., Chiba (Japan))</au><au>Kimizuka Takagi, C</au><au>Ishii, K</au><au>Nomura, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea</atitle><jtitle>Breeding Science</jtitle><addtitle>Breeding Science</addtitle><date>1999</date><risdate>1999</risdate><volume>49</volume><issue>4</issue><spage>257</spage><epage>265</epage><pages>257-265</pages><issn>1344-7610</issn><eissn>1347-3735</eissn><abstract>We analyzed 124 markers for linkage in 138 F2 plants derived from a cross between 2 inbred lines, cabbage and kale (both Brassica oleracea). Ninety-nine RAPDs, 21 RFLPs, 2 isozyme genes, and 2 morphological characters were used to construct a genetic map spanning 9 linkage groups covering 823.6 cM of the B. oleracea genome. The number of mapped loci for each linkage group ranged from 9 to 23. The average interval between 2 loci was 7.16 cM. The maximum distance between two loci in the linkage groups was 283 cM. Most of the markers segregated in a normal Mendelian ratio. However, biased segregation was observed at 10 loci. Most of the loci were clustered in a particular area of the genome, and showed an over-abundance of the male parent, indicating the presence of a lethal allele in the neighborhood of these markers or a quantitative selection advantage for one or more alleles. One QTL for resistance to clubroot disease was identified. QTL mapping confirmed the identification of the probable location and effect of a QTL on linkage group 3. The QTL was separated from the nearest marker by a distance of 1.0 cM, and its R2 value was 0.300 indicating phenotypic variation</abstract><cop>Tokyo</cop><pub>Japanese Society of Breeding</pub><doi>10.1270/jsbbs.49.257</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Agronomy. Soil science and plant productions ANALISIS MICROBIOLOGICO ANALYSE MICROBIOLOGIQUE Biological and medical sciences BRASSICA OLERACEA CARTE GENETIQUE DISEASE RESISTANCE Fundamental and applied biological sciences. Psychology GENETIC MAPS Genetics and breeding of economic plants ISOENZIMAS ISOENZYME ISOENZYMES MAPAS GENETICOS METHODE STATISTIQUE METODOS ESTADISTICOS MICROBIOLOGICAL ANALYSIS MORFOGENESIS MORPHOGENESE MORPHOGENESIS Pest resistance Plant pathogens PLASMODIOPHORA BRASSICAE RAPD RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD RFLP STATISTICAL METHODS Varietal selection. Specialized plant breeding, plant breeding aims |
| title | A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea |
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