XENOTRANSPLANTED IN VIVO CULTURE MODEL OF MUCOUS CELL HYPERPLASIA IN RESPIRATORY EPITHELIUM INDUCED BY HUMAN NEUTROPHIL ELASTASE
This study was conducted to establish a novel model of airway mucous cell hyperplasia and mucous hypersecretion suitable for both morphological and physico-biochemical examinations, using a xenotransplanted in vivo culture system. Tracheal epithelial cells of rabbits were cultured in de-epith-eliali...
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| Veröffentlicht in: | Journal of Toxicologic Pathology 1995/03/31, Vol.8(1), pp.15-24 |
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| container_title | Journal of Toxicologic Pathology |
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| creator | Asano, Satoshi Inayama, Yoshiaki Kitamura, Hitoshi Kanisawa, Masayoshi Nakatani, Yukio Izawa, Yoshihiro Nettesheim, Paul |
| description | This study was conducted to establish a novel model of airway mucous cell hyperplasia and mucous hypersecretion suitable for both morphological and physico-biochemical examinations, using a xenotransplanted in vivo culture system. Tracheal epithelial cells of rabbits were cultured in de-epith-elialized rat tracheal grafts transplanted to nude mice. At four weeks, human neutrophil elastase (300μg/graft) or saline was injected into the graft lumina. Three weeks later, intratracheal mucus was collected from the retrieved tracheas, and the grafts were examined histologically. The percentage of mucous cells in the elastase-treated tracheas was significantly increased compared to the control level (25.5±0.8% vs. 12.9±0.9%, p |
| doi_str_mv | 10.1293/tox.8.15 |
| format | Article |
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Tracheal epithelial cells of rabbits were cultured in de-epith-elialized rat tracheal grafts transplanted to nude mice. At four weeks, human neutrophil elastase (300μg/graft) or saline was injected into the graft lumina. Three weeks later, intratracheal mucus was collected from the retrieved tracheas, and the grafts were examined histologically. The percentage of mucous cells in the elastase-treated tracheas was significantly increased compared to the control level (25.5±0.8% vs. 12.9±0.9%, p<0.001). Histochemically, mucous cells containing acidic glycoprotein were more abundant in the elastase-treated tracheas. The volume of mucus did not differ in the untreated and treated tracheas. The acidity of the mucus in the control and the elastasetreated tracheas was pH 7.7±0.2 and 6.3±0.1, respectively (p<0.001). Amounts of protein, N-acetyl-neuraminic acid, and fucose were all significantly higher in the elastase-treated tracheas. These results indicate that elastase induced mucous cell hyperplasia and the hypersecretion of acidic mucus. This culture model will be useful for studying the role played by elastase in the development of this pathological condition.</description><identifier>ISSN: 0914-9198</identifier><identifier>EISSN: 1881-915X</identifier><identifier>EISSN: 1347-7404</identifier><identifier>DOI: 10.1293/tox.8.15</identifier><language>eng</language><publisher>Tokyo: JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY</publisher><subject>Mucous cell hyperplasia ; Mucous hypersecretion ; Neutrophil elastase ; Tracheal epithelium ; Xenotransplant</subject><ispartof>Journal of Toxicologic Pathology, 1995/03/31, Vol.8(1), pp.15-24</ispartof><rights>The Japanese Society of Toxicologic Pathology</rights><rights>Copyright Japan Science and Technology Agency 1995</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,1879,4012,27912,27913,27914</link.rule.ids></links><search><creatorcontrib>Asano, Satoshi</creatorcontrib><creatorcontrib>Inayama, Yoshiaki</creatorcontrib><creatorcontrib>Kitamura, Hitoshi</creatorcontrib><creatorcontrib>Kanisawa, Masayoshi</creatorcontrib><creatorcontrib>Nakatani, Yukio</creatorcontrib><creatorcontrib>Izawa, Yoshihiro</creatorcontrib><creatorcontrib>Nettesheim, Paul</creatorcontrib><title>XENOTRANSPLANTED IN VIVO CULTURE MODEL OF MUCOUS CELL HYPERPLASIA IN RESPIRATORY EPITHELIUM INDUCED BY HUMAN NEUTROPHIL ELASTASE</title><title>Journal of Toxicologic Pathology</title><addtitle>J Toxicol Pathol</addtitle><description>This study was conducted to establish a novel model of airway mucous cell hyperplasia and mucous hypersecretion suitable for both morphological and physico-biochemical examinations, using a xenotransplanted in vivo culture system. Tracheal epithelial cells of rabbits were cultured in de-epith-elialized rat tracheal grafts transplanted to nude mice. At four weeks, human neutrophil elastase (300μg/graft) or saline was injected into the graft lumina. Three weeks later, intratracheal mucus was collected from the retrieved tracheas, and the grafts were examined histologically. The percentage of mucous cells in the elastase-treated tracheas was significantly increased compared to the control level (25.5±0.8% vs. 12.9±0.9%, p<0.001). Histochemically, mucous cells containing acidic glycoprotein were more abundant in the elastase-treated tracheas. The volume of mucus did not differ in the untreated and treated tracheas. The acidity of the mucus in the control and the elastasetreated tracheas was pH 7.7±0.2 and 6.3±0.1, respectively (p<0.001). Amounts of protein, N-acetyl-neuraminic acid, and fucose were all significantly higher in the elastase-treated tracheas. These results indicate that elastase induced mucous cell hyperplasia and the hypersecretion of acidic mucus. This culture model will be useful for studying the role played by elastase in the development of this pathological condition.</description><subject>Mucous cell hyperplasia</subject><subject>Mucous hypersecretion</subject><subject>Neutrophil elastase</subject><subject>Tracheal epithelium</subject><subject>Xenotransplant</subject><issn>0914-9198</issn><issn>1881-915X</issn><issn>1347-7404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNo9kF9LwzAUxYMoOKfgRwj44stm0r_Jm7XLbCFrS9uM7SmkNVGHutluoG9-dDMne7jcC-d37uUeAK4xGmOHunfb9deYjLF_AgaYEDyi2F-cggGi2LMzJefgou9XCDkh8t0B-FmwLK_LKKsKHmU1m8A0g_N0nsNY8FqUDM7yCeMwn8KZiHNRwZhxDpNlwUrrqNJobyhZVaRlVOflErIirRPGUzGzykTEduXDEiZiFmUwY6Iu8yJJOWTWXEcVuwRnRr31-uq_D4GYsjpORjx_TOOIj1qXhP7IRx6hjXZV8xQSD4daIaPahqrGmACbViNHPynquco0QeC1SmnjGqyM73jEfusOwc1h76Zbf-50v5Wr9a77sCcl9oLQw5Q6gaVuD1Tbrfu-00Zuutd31X1LjOQ-X2nzlURi36L3B3TVb9WzPoKq2762b3oP2rjJHv4r7B-l9kV1Un-4v8IlfEQ</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Asano, Satoshi</creator><creator>Inayama, Yoshiaki</creator><creator>Kitamura, Hitoshi</creator><creator>Kanisawa, Masayoshi</creator><creator>Nakatani, Yukio</creator><creator>Izawa, Yoshihiro</creator><creator>Nettesheim, Paul</creator><general>JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>1995</creationdate><title>XENOTRANSPLANTED IN VIVO CULTURE MODEL OF MUCOUS CELL HYPERPLASIA IN RESPIRATORY EPITHELIUM INDUCED BY HUMAN NEUTROPHIL ELASTASE</title><author>Asano, Satoshi ; Inayama, Yoshiaki ; Kitamura, Hitoshi ; Kanisawa, Masayoshi ; Nakatani, Yukio ; Izawa, Yoshihiro ; Nettesheim, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3875-50489be3abd78417ea0facb9abff61fce02eda943afb664caaef3f1af52480023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Mucous cell hyperplasia</topic><topic>Mucous hypersecretion</topic><topic>Neutrophil elastase</topic><topic>Tracheal epithelium</topic><topic>Xenotransplant</topic><toplevel>online_resources</toplevel><creatorcontrib>Asano, Satoshi</creatorcontrib><creatorcontrib>Inayama, Yoshiaki</creatorcontrib><creatorcontrib>Kitamura, Hitoshi</creatorcontrib><creatorcontrib>Kanisawa, Masayoshi</creatorcontrib><creatorcontrib>Nakatani, Yukio</creatorcontrib><creatorcontrib>Izawa, Yoshihiro</creatorcontrib><creatorcontrib>Nettesheim, Paul</creatorcontrib><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of Toxicologic Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asano, Satoshi</au><au>Inayama, Yoshiaki</au><au>Kitamura, Hitoshi</au><au>Kanisawa, Masayoshi</au><au>Nakatani, Yukio</au><au>Izawa, Yoshihiro</au><au>Nettesheim, Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>XENOTRANSPLANTED IN VIVO CULTURE MODEL OF MUCOUS CELL HYPERPLASIA IN RESPIRATORY EPITHELIUM INDUCED BY HUMAN NEUTROPHIL ELASTASE</atitle><jtitle>Journal of Toxicologic Pathology</jtitle><addtitle>J Toxicol Pathol</addtitle><date>1995</date><risdate>1995</risdate><volume>8</volume><issue>1</issue><spage>15</spage><epage>24</epage><pages>15-24</pages><issn>0914-9198</issn><eissn>1881-915X</eissn><eissn>1347-7404</eissn><abstract>This study was conducted to establish a novel model of airway mucous cell hyperplasia and mucous hypersecretion suitable for both morphological and physico-biochemical examinations, using a xenotransplanted in vivo culture system. Tracheal epithelial cells of rabbits were cultured in de-epith-elialized rat tracheal grafts transplanted to nude mice. At four weeks, human neutrophil elastase (300μg/graft) or saline was injected into the graft lumina. Three weeks later, intratracheal mucus was collected from the retrieved tracheas, and the grafts were examined histologically. The percentage of mucous cells in the elastase-treated tracheas was significantly increased compared to the control level (25.5±0.8% vs. 12.9±0.9%, p<0.001). Histochemically, mucous cells containing acidic glycoprotein were more abundant in the elastase-treated tracheas. The volume of mucus did not differ in the untreated and treated tracheas. The acidity of the mucus in the control and the elastasetreated tracheas was pH 7.7±0.2 and 6.3±0.1, respectively (p<0.001). Amounts of protein, N-acetyl-neuraminic acid, and fucose were all significantly higher in the elastase-treated tracheas. These results indicate that elastase induced mucous cell hyperplasia and the hypersecretion of acidic mucus. This culture model will be useful for studying the role played by elastase in the development of this pathological condition.</abstract><cop>Tokyo</cop><pub>JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY</pub><doi>10.1293/tox.8.15</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0914-9198 |
| ispartof | Journal of Toxicologic Pathology, 1995/03/31, Vol.8(1), pp.15-24 |
| issn | 0914-9198 1881-915X 1347-7404 |
| language | eng |
| recordid | cdi_proquest_journals_1467419926 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Mucous cell hyperplasia Mucous hypersecretion Neutrophil elastase Tracheal epithelium Xenotransplant |
| title | XENOTRANSPLANTED IN VIVO CULTURE MODEL OF MUCOUS CELL HYPERPLASIA IN RESPIRATORY EPITHELIUM INDUCED BY HUMAN NEUTROPHIL ELASTASE |
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