Preparation of Antibody against Antitumor [beta]-Glucan in Grifola frondosa and Its Application

Preparation of Antibody against Antitumor [beta]-Glucan in Grifola frondosa and Its Application

Antibodies against an antitumor β-glucan purified from Grifola frondosa (GGF) were raised in the rabbit by subcutaneous immunization. Our antibodies reacted significantly with GGF by an ELISA inhibition assay. The antibodies did not recognize other polysaccharides such as laminarin and pustulan, but...

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Veröffentlicht in:Food science and technology research 1999-10, Vol.5 (4), p.398
Hauptverfasser: MIZUNO, Masashi, YAMAKAWA, Akio, MINATO, Ken-ichiro, KAWAKAMI, Sachiko, TATSUOKA, Shigenobu, TERAI, Hirofumi, TSUCHIDA, Hironobu
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container_issue 4
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container_title Food science and technology research
container_volume 5
creator MIZUNO, Masashi
YAMAKAWA, Akio
MINATO, Ken-ichiro
KAWAKAMI, Sachiko
TATSUOKA, Shigenobu
TERAI, Hirofumi
TSUCHIDA, Hironobu
description Antibodies against an antitumor β-glucan purified from Grifola frondosa (GGF) were raised in the rabbit by subcutaneous immunization. Our antibodies reacted significantly with GGF by an ELISA inhibition assay. The antibodies did not recognize other polysaccharides such as laminarin and pustulan, but reacted somewhat with lentinan, whose structure is similar to GGF. It was demonstrated that GGF could be measured by ELISA using antibodies. In addition, the effects of the storage temperature on GGF content during storage were measured using our antibody. GGF content was 24.7 μg/g fresh weight (f.w.) at zero time storage, and little change occurred during storage of the mushroom for 7 days at 5°C. However, a drastic decrease to 11.4 μg/g f.w. occurred after 7 days of storage at 20°C. These results suggest that storage at low temperatures is desirable to maintain the quality of GGF.
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