Fabrication of avidin single molecular layer on silicon oxide surfaces and formation of tethered lipid bilayer membranes
Single molecular layer of avidin is fabricated on an atomically flat SiO2 surface and characterized by atomic force microscopy (AFM) and infrared reflection absorption spectroscopy. Immobilization of avidin is performed as follows; i) ester-modification of the surface by silane-coupling agent, ii) c...
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| Veröffentlicht in: | E-journal of surface science and nanotechnology 2005, Vol.3, pp.237-243 |
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| container_title | E-journal of surface science and nanotechnology |
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| creator | Tero, R. Misawa, N. Watanabe, H. Yamamura, S. Nambu, S. Nonogaki, Y. Urisu, T. |
| description | Single molecular layer of avidin is fabricated on an atomically flat SiO2 surface and characterized by atomic force microscopy (AFM) and infrared reflection absorption spectroscopy. Immobilization of avidin is performed as follows; i) ester-modification of the surface by silane-coupling agent, ii) carboxylation by hydrolysis in HCl and iii) amide bonding between the surface -COOH and -NH2 in avidin molecules. Large dome structures (∼60 nm height) are formed after the ester-modification, but an atomically flat surface is obtained after the hydrolysis reaction. AFM topographs and function-recognizing images show that the each of avidin molecules adsorbs as a single molecule and retains the biotin-binding activity. Formation of a tethered bilayer membrane of a biotinylated phospholipid on the avidin layer is also described. [DOI: 10.1380/ejssnt.2005.237] |
| doi_str_mv | 10.1380/ejssnt.2005.237 |
| format | Article |
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Immobilization of avidin is performed as follows; i) ester-modification of the surface by silane-coupling agent, ii) carboxylation by hydrolysis in HCl and iii) amide bonding between the surface -COOH and -NH2 in avidin molecules. Large dome structures (∼60 nm height) are formed after the ester-modification, but an atomically flat surface is obtained after the hydrolysis reaction. AFM topographs and function-recognizing images show that the each of avidin molecules adsorbs as a single molecule and retains the biotin-binding activity. Formation of a tethered bilayer membrane of a biotinylated phospholipid on the avidin layer is also described. 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Surf. Sci. Nanotechnol.</addtitle><description>Single molecular layer of avidin is fabricated on an atomically flat SiO2 surface and characterized by atomic force microscopy (AFM) and infrared reflection absorption spectroscopy. Immobilization of avidin is performed as follows; i) ester-modification of the surface by silane-coupling agent, ii) carboxylation by hydrolysis in HCl and iii) amide bonding between the surface -COOH and -NH2 in avidin molecules. Large dome structures (∼60 nm height) are formed after the ester-modification, but an atomically flat surface is obtained after the hydrolysis reaction. AFM topographs and function-recognizing images show that the each of avidin molecules adsorbs as a single molecule and retains the biotin-binding activity. Formation of a tethered bilayer membrane of a biotinylated phospholipid on the avidin layer is also described. [DOI: 10.1380/ejssnt.2005.237]</description><subject>Ab initio quantum chemical methods and calculations</subject><subject>Atomic force microscopy</subject><subject>Lipid bilayers</subject><subject>Molecular recognition</subject><subject>Protein immobilization</subject><subject>Silicon oxides</subject><subject>Surface chemical reaction</subject><subject>Surface structure, morphology,roughness, and topography</subject><issn>1348-0391</issn><issn>1348-0391</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNpNkM1LxDAQxYsouK6evQY8dzdp2rS9KYtfsOBFz2GaTHdT2mRNurL739taXTwMM_B-7w28KLpldMF4QZfYhGD7RUJptkh4fhbNGE-LmPKSnf-7L6OrEBpKec5zMYsOT1B5o6A3zhJXE_gy2lgSjN20SDrXotq34EkLR_TEjUpr1MgejEYS9r4GhYGA1aR2vjsF9dhv0aMmrdkZTSozJXTYVR4shuvoooY24M3vnkcfT4_vq5d4_fb8unpYxyrjaR8jz3VBE50xKFOFCc9YWohcFFVWF1UJvCqAlzrPs6QUAkAoEIImQhVaJ1RrPo_uptydd597DL1s3N7b4aVkqeAsYUzQgVpOlPIuBI-13HnTgT9KRuVYr5zqlWO9cqh3cNxPjib0sMETD743qsU_nkv6M4PlJKkteImWfwNdgIjX</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Tero, R.</creator><creator>Misawa, N.</creator><creator>Watanabe, H.</creator><creator>Yamamura, S.</creator><creator>Nambu, S.</creator><creator>Nonogaki, Y.</creator><creator>Urisu, T.</creator><general>The Japan Society of Vacuum and Surface Science</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20050101</creationdate><title>Fabrication of avidin single molecular layer on silicon oxide surfaces and formation of tethered lipid bilayer membranes</title><author>Tero, R. ; Misawa, N. ; Watanabe, H. ; Yamamura, S. ; Nambu, S. ; Nonogaki, Y. ; Urisu, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-e37d802d51a94ce2351486768b5f8b9a3b8a39d7752966aa6ca66026c8dd20dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Ab initio quantum chemical methods and calculations</topic><topic>Atomic force microscopy</topic><topic>Lipid bilayers</topic><topic>Molecular recognition</topic><topic>Protein immobilization</topic><topic>Silicon oxides</topic><topic>Surface chemical reaction</topic><topic>Surface structure, morphology,roughness, and topography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tero, R.</creatorcontrib><creatorcontrib>Misawa, N.</creatorcontrib><creatorcontrib>Watanabe, H.</creatorcontrib><creatorcontrib>Yamamura, S.</creatorcontrib><creatorcontrib>Nambu, S.</creatorcontrib><creatorcontrib>Nonogaki, Y.</creatorcontrib><creatorcontrib>Urisu, T.</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>E-journal of surface science and nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tero, R.</au><au>Misawa, N.</au><au>Watanabe, H.</au><au>Yamamura, S.</au><au>Nambu, S.</au><au>Nonogaki, Y.</au><au>Urisu, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication of avidin single molecular layer on silicon oxide surfaces and formation of tethered lipid bilayer membranes</atitle><jtitle>E-journal of surface science and nanotechnology</jtitle><addtitle>e-J. Surf. Sci. Nanotechnol.</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>3</volume><spage>237</spage><epage>243</epage><pages>237-243</pages><issn>1348-0391</issn><eissn>1348-0391</eissn><abstract>Single molecular layer of avidin is fabricated on an atomically flat SiO2 surface and characterized by atomic force microscopy (AFM) and infrared reflection absorption spectroscopy. Immobilization of avidin is performed as follows; i) ester-modification of the surface by silane-coupling agent, ii) carboxylation by hydrolysis in HCl and iii) amide bonding between the surface -COOH and -NH2 in avidin molecules. Large dome structures (∼60 nm height) are formed after the ester-modification, but an atomically flat surface is obtained after the hydrolysis reaction. AFM topographs and function-recognizing images show that the each of avidin molecules adsorbs as a single molecule and retains the biotin-binding activity. Formation of a tethered bilayer membrane of a biotinylated phospholipid on the avidin layer is also described. [DOI: 10.1380/ejssnt.2005.237]</abstract><cop>Tokyo</cop><pub>The Japan Society of Vacuum and Surface Science</pub><doi>10.1380/ejssnt.2005.237</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Ab initio quantum chemical methods and calculations Atomic force microscopy Lipid bilayers Molecular recognition Protein immobilization Silicon oxides Surface chemical reaction Surface structure, morphology,roughness, and topography |
| title | Fabrication of avidin single molecular layer on silicon oxide surfaces and formation of tethered lipid bilayer membranes |
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