Comprehensive transcriptional profiling of NaHCO₃-stressed Tamarix hispida roots reveals networks of responsive genes

Root tissue is the primary site of perception for stress from soil, and is the main tissue involved in stress response. Tamarix hispida is a woody halophyte that is highly tolerant to salt and drought stress, but little information available about gene expression in roots in response to abiotic stre...

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Veröffentlicht in:Plant molecular biology 2014-01, Vol.84 (1-2), p.145-157
Hauptverfasser: Wang, Chao, Gao, Caiqiu, Wang, Liuqiang, Zheng, Lei, Yang, Chuanping, Wang, Yucheng
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container_issue 1-2
container_start_page 145
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creator Wang, Chao
Gao, Caiqiu
Wang, Liuqiang
Zheng, Lei
Yang, Chuanping
Wang, Yucheng
description Root tissue is the primary site of perception for stress from soil, and is the main tissue involved in stress response. Tamarix hispida is a woody halophyte that is highly tolerant to salt and drought stress, but little information available about gene expression in roots in response to abiotic stress. In this study, eight transcriptomes from roots of T. hispida treated with NaHCO₃ for 0, 12, 24 and 48 h (two biological replicates were set at each time point) were built. In total, 47,324 unigenes were generated, and 6,267 differentially expressed genes (DEGs) were identified. There were 2,510, 3,690, and 2,636 genes significantly differentially expressed after stress for 12, 24 and 48 h, respectively. Co-expressed DEGs were clustered into ten classes (P < 0.001). Gene ontology enrichment analysis showed that 13 pathways were highly enriched, such as signal transduction, cell wall, phosphatase activity, and lipid kinase activity, suggesting that these pathways play important roles in the saline–alkaline response. Furthermore, the genes involved in lignin metabolic processes and biosynthesis of proline and trehalose are found closely involved in NaHCO₃ stress response. This systematic analysis may provide an in-depth view of stress tolerance mechanisms in T. hispida.
doi_str_mv 10.1007/s11103-013-0124-2
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Tamarix hispida is a woody halophyte that is highly tolerant to salt and drought stress, but little information available about gene expression in roots in response to abiotic stress. In this study, eight transcriptomes from roots of T. hispida treated with NaHCO₃ for 0, 12, 24 and 48 h (two biological replicates were set at each time point) were built. In total, 47,324 unigenes were generated, and 6,267 differentially expressed genes (DEGs) were identified. There were 2,510, 3,690, and 2,636 genes significantly differentially expressed after stress for 12, 24 and 48 h, respectively. Co-expressed DEGs were clustered into ten classes (P &lt; 0.001). Gene ontology enrichment analysis showed that 13 pathways were highly enriched, such as signal transduction, cell wall, phosphatase activity, and lipid kinase activity, suggesting that these pathways play important roles in the saline–alkaline response. 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subjects Abiotic stress
Biochemistry
Biomedical and Life Sciences
Biosynthesis
cell walls
Drought
Gene expression
gene expression regulation
Life Sciences
Plant biology
Plant Pathology
Plant Sciences
Plant tissues
Plant tolerance
Roots
salt tolerance
Signal transduction
stress response
stress tolerance
Tamarix
transcription (genetics)
transcriptome
trehalose
unigenes
water stress
title Comprehensive transcriptional profiling of NaHCO₃-stressed Tamarix hispida roots reveals networks of responsive genes
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