key role of a non-active-site residue Met148 on the catalytic efficiency of meta-cleavage product hydrolase BphD

meta-Cleavage product (MCP) hydrolases (EC 3.7.1.9) can catalyze a specific C–C bond fission during the microbial aerobic degradation of aromatics. The previous studies on structure–function relationship of MCP hydrolases mainly focus on the active site residues by site-directed mutagenesis. However...

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Veröffentlicht in:	Applied microbiology and biotechnology 2013-12, Vol.97 (24), p.10399-10411
Hauptverfasser:	Zhou, Hao, Qu, Yuanyuan, Kong, Chunlei, Shen, E, Wang, Jingwei, Zhang, Xuwang, Ma, Qiao, Zhou, Jiti
Format:	Artikel
Sprache:	eng
Schlagworte:	Aerobic conditions Amino acids Analysis Biocatalysts Biodegradation Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Carbon cycle Chemical properties cluster analysis DNA Mutational Analysis E coli Efficiency Enzymes Fatty Acids, Unsaturated - metabolism Hydrolases Hydrolases - chemistry Hydrolases - genetics Hydrolases - metabolism Kinetics Life Sciences Microbial Genetics and Genomics Microbiology molecular dynamics Molecular Dynamics Simulation Mutagenesis Mutagenesis, Site-Directed Mutant Proteins - chemistry Mutant Proteins - genetics Mutant Proteins - metabolism Mutants Mutation, Missense Plasmids Proteins Protons Residues site-directed mutagenesis Studies
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