Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream
Background Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC). Aim The purpose of this study was to evaluate the antioxidants effects of n -acetylcysteine (NAC) in colon segments without faecal stream. Methods Thirty-six Wistar rats were s...
Gespeichert in:
| Veröffentlicht in: | Digestive diseases and sciences 2013-12, Vol.58 (12), p.3452-3459 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3459 |
|---|---|
| container_issue | 12 |
| container_start_page | 3452 |
| container_title | Digestive diseases and sciences |
| container_volume | 58 |
| creator | Martinez, Carlos Augusto Real de Almeida, Marcos Gonçalves da Silva, Camila Moraes Gonçalves Ribeiro, Marcelo Lima da Cunha, Fernando Lorenzetti Rodrigues, Murilo Rocha Sato, Daniela Tiemi Pereira, José Aires |
| description | Background
Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC).
Aim
The purpose of this study was to evaluate the antioxidants effects of
n
-acetylcysteine (NAC) in colon segments without faecal stream.
Methods
Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann–Whitney test and ANOVA were used for statistical analysis;
p
|
| doi_str_mv | 10.1007/s10620-013-2768-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_1460838939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A712943063</galeid><sourcerecordid>A712943063</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-da047e3666de9241e37e295e94d29f6f44d8bb58f396fa2499f74a0f9f8885f03</originalsourceid><addsrcrecordid>eNp1kU1v1DAQhiMEokvhB3BBljin-CuOfVxtW0BaVImPs-V1xltXiV1sp7B3fjgOW74kkA_j8TzveEZv0zwn-Ixg3L_KBAuKW0xYS3shW_WgWZGur1kn5MNmhYmod0LESfMk5xuMseqJeNycUCapJJytmm8XASaT0RdfrlFo1xbKYbSHXMAHQBsT0HsYZguoXAPawh2MKDp09dUPpvg7QOdmMntAPqANjGNeigu5iWMM3qJ3s43ZoPOKpgIDcilOP4BLA9aM6ENJYKanzSNnxgzP7uNp8-ny4uPmTbu9ev12s962ljNV2sFg3gMTQgygKCfAeqCqA8UHqpxwnA9yt-ukY0o4Q7lSrucGO-WklJ3D7LR5eex7m-LnGXLRN3FOoX6pCRdYMqmY-k3tzQjaBxdLMnby2ep1T6jiDAtWqbN_UPUMMHkbAzhf3_8SkKPApphzAqdvk59MOmiC9WKnPtqpq516sVMvo7y4H3jeTTD8Uvz0rwL0CORaCntIf2z0367fAc-EqGM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1460838939</pqid></control><display><type>article</type><title>Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Martinez, Carlos Augusto Real ; de Almeida, Marcos Gonçalves ; da Silva, Camila Moraes Gonçalves ; Ribeiro, Marcelo Lima ; da Cunha, Fernando Lorenzetti ; Rodrigues, Murilo Rocha ; Sato, Daniela Tiemi ; Pereira, José Aires</creator><creatorcontrib>Martinez, Carlos Augusto Real ; de Almeida, Marcos Gonçalves ; da Silva, Camila Moraes Gonçalves ; Ribeiro, Marcelo Lima ; da Cunha, Fernando Lorenzetti ; Rodrigues, Murilo Rocha ; Sato, Daniela Tiemi ; Pereira, José Aires</creatorcontrib><description>Background
Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC).
Aim
The purpose of this study was to evaluate the antioxidants effects of
n
-acetylcysteine (NAC) in colon segments without faecal stream.
Methods
Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann–Whitney test and ANOVA were used for statistical analysis;
p
< 0.05 was considered significant.
Results
The oxidative DNA damage in colon segments without faecal stream was significantly lower in animals treated with either concentration of NAC than in control group, regardless of the duration of intervention (
p
< 0.01).
Conclusions
Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.</description><identifier>ISSN: 0163-2116</identifier><identifier>EISSN: 1573-2568</identifier><identifier>DOI: 10.1007/s10620-013-2768-9</identifier><identifier>PMID: 23828143</identifier><identifier>CODEN: DDSCDJ</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Acetylcysteine ; Acetylcysteine - administration & dosage ; Analysis ; Animals ; Antioxidants ; Biochemistry ; Colectomy - adverse effects ; Colitis ; Colitis - drug therapy ; Colitis - etiology ; Colitis - metabolism ; Colon - pathology ; DNA Damage - drug effects ; Enema ; Free Radical Scavengers - administration & dosage ; Gastroenterology ; Hepatology ; Inflammation ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Medicine ; Medicine & Public Health ; Oncology ; Original Article ; Oxidative Stress - drug effects ; Pouchitis - drug therapy ; Random Allocation ; Rats ; Rats, Wistar ; Transplant Surgery</subject><ispartof>Digestive diseases and sciences, 2013-12, Vol.58 (12), p.3452-3459</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>COPYRIGHT 2013 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-da047e3666de9241e37e295e94d29f6f44d8bb58f396fa2499f74a0f9f8885f03</citedby><cites>FETCH-LOGICAL-c439t-da047e3666de9241e37e295e94d29f6f44d8bb58f396fa2499f74a0f9f8885f03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10620-013-2768-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10620-013-2768-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23828143$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Martinez, Carlos Augusto Real</creatorcontrib><creatorcontrib>de Almeida, Marcos Gonçalves</creatorcontrib><creatorcontrib>da Silva, Camila Moraes Gonçalves</creatorcontrib><creatorcontrib>Ribeiro, Marcelo Lima</creatorcontrib><creatorcontrib>da Cunha, Fernando Lorenzetti</creatorcontrib><creatorcontrib>Rodrigues, Murilo Rocha</creatorcontrib><creatorcontrib>Sato, Daniela Tiemi</creatorcontrib><creatorcontrib>Pereira, José Aires</creatorcontrib><title>Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream</title><title>Digestive diseases and sciences</title><addtitle>Dig Dis Sci</addtitle><addtitle>Dig Dis Sci</addtitle><description>Background
Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC).
Aim
The purpose of this study was to evaluate the antioxidants effects of
n
-acetylcysteine (NAC) in colon segments without faecal stream.
Methods
Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann–Whitney test and ANOVA were used for statistical analysis;
p
< 0.05 was considered significant.
Results
The oxidative DNA damage in colon segments without faecal stream was significantly lower in animals treated with either concentration of NAC than in control group, regardless of the duration of intervention (
p
< 0.01).
Conclusions
Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.</description><subject>Acetylcysteine</subject><subject>Acetylcysteine - administration & dosage</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Colectomy - adverse effects</subject><subject>Colitis</subject><subject>Colitis - drug therapy</subject><subject>Colitis - etiology</subject><subject>Colitis - metabolism</subject><subject>Colon - pathology</subject><subject>DNA Damage - drug effects</subject><subject>Enema</subject><subject>Free Radical Scavengers - administration & dosage</subject><subject>Gastroenterology</subject><subject>Hepatology</subject><subject>Inflammation</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article</subject><subject>Oxidative Stress - drug effects</subject><subject>Pouchitis - drug therapy</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Transplant Surgery</subject><issn>0163-2116</issn><issn>1573-2568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kU1v1DAQhiMEokvhB3BBljin-CuOfVxtW0BaVImPs-V1xltXiV1sp7B3fjgOW74kkA_j8TzveEZv0zwn-Ixg3L_KBAuKW0xYS3shW_WgWZGur1kn5MNmhYmod0LESfMk5xuMseqJeNycUCapJJytmm8XASaT0RdfrlFo1xbKYbSHXMAHQBsT0HsYZguoXAPawh2MKDp09dUPpvg7QOdmMntAPqANjGNeigu5iWMM3qJ3s43ZoPOKpgIDcilOP4BLA9aM6ENJYKanzSNnxgzP7uNp8-ny4uPmTbu9ev12s962ljNV2sFg3gMTQgygKCfAeqCqA8UHqpxwnA9yt-ukY0o4Q7lSrucGO-WklJ3D7LR5eex7m-LnGXLRN3FOoX6pCRdYMqmY-k3tzQjaBxdLMnby2ep1T6jiDAtWqbN_UPUMMHkbAzhf3_8SkKPApphzAqdvk59MOmiC9WKnPtqpq516sVMvo7y4H3jeTTD8Uvz0rwL0CORaCntIf2z0367fAc-EqGM</recordid><startdate>20131201</startdate><enddate>20131201</enddate><creator>Martinez, Carlos Augusto Real</creator><creator>de Almeida, Marcos Gonçalves</creator><creator>da Silva, Camila Moraes Gonçalves</creator><creator>Ribeiro, Marcelo Lima</creator><creator>da Cunha, Fernando Lorenzetti</creator><creator>Rodrigues, Murilo Rocha</creator><creator>Sato, Daniela Tiemi</creator><creator>Pereira, José Aires</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20131201</creationdate><title>Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream</title><author>Martinez, Carlos Augusto Real ; de Almeida, Marcos Gonçalves ; da Silva, Camila Moraes Gonçalves ; Ribeiro, Marcelo Lima ; da Cunha, Fernando Lorenzetti ; Rodrigues, Murilo Rocha ; Sato, Daniela Tiemi ; Pereira, José Aires</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-da047e3666de9241e37e295e94d29f6f44d8bb58f396fa2499f74a0f9f8885f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acetylcysteine</topic><topic>Acetylcysteine - administration & dosage</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Colectomy - adverse effects</topic><topic>Colitis</topic><topic>Colitis - drug therapy</topic><topic>Colitis - etiology</topic><topic>Colitis - metabolism</topic><topic>Colon - pathology</topic><topic>DNA Damage - drug effects</topic><topic>Enema</topic><topic>Free Radical Scavengers - administration & dosage</topic><topic>Gastroenterology</topic><topic>Hepatology</topic><topic>Inflammation</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Original Article</topic><topic>Oxidative Stress - drug effects</topic><topic>Pouchitis - drug therapy</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Transplant Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Martinez, Carlos Augusto Real</creatorcontrib><creatorcontrib>de Almeida, Marcos Gonçalves</creatorcontrib><creatorcontrib>da Silva, Camila Moraes Gonçalves</creatorcontrib><creatorcontrib>Ribeiro, Marcelo Lima</creatorcontrib><creatorcontrib>da Cunha, Fernando Lorenzetti</creatorcontrib><creatorcontrib>Rodrigues, Murilo Rocha</creatorcontrib><creatorcontrib>Sato, Daniela Tiemi</creatorcontrib><creatorcontrib>Pereira, José Aires</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Digestive diseases and sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Martinez, Carlos Augusto Real</au><au>de Almeida, Marcos Gonçalves</au><au>da Silva, Camila Moraes Gonçalves</au><au>Ribeiro, Marcelo Lima</au><au>da Cunha, Fernando Lorenzetti</au><au>Rodrigues, Murilo Rocha</au><au>Sato, Daniela Tiemi</au><au>Pereira, José Aires</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream</atitle><jtitle>Digestive diseases and sciences</jtitle><stitle>Dig Dis Sci</stitle><addtitle>Dig Dis Sci</addtitle><date>2013-12-01</date><risdate>2013</risdate><volume>58</volume><issue>12</issue><spage>3452</spage><epage>3459</epage><pages>3452-3459</pages><issn>0163-2116</issn><eissn>1573-2568</eissn><coden>DDSCDJ</coden><abstract>Background
Oxidative stress has been related to inflammation of the colonic mucosa in patients with diversion colitis (DC).
Aim
The purpose of this study was to evaluate the antioxidants effects of
n
-acetylcysteine (NAC) in colon segments without faecal stream.
Methods
Thirty-six Wistar rats were subjected to diversion of the faecal stream by proximal colostomy and a distal mucosal colon fistula. They were distributed into three experimental groups of 12 animals each; the animals in each group underwent daily enemas containing saline solution (control group) or either a 25 or 100 mg/kg dose of NAC (treated groups). In each group, animals were sacrificed after 2 or 4 weeks. The degree of inflammation was determined by histopathological analysis and stratified by inflammatory grading scale. Oxidative DNA damage was measured by comet assay. The Mann–Whitney test and ANOVA were used for statistical analysis;
p
< 0.05 was considered significant.
Results
The oxidative DNA damage in colon segments without faecal stream was significantly lower in animals treated with either concentration of NAC than in control group, regardless of the duration of intervention (
p
< 0.01).
Conclusions
Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23828143</pmid><doi>10.1007/s10620-013-2768-9</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0163-2116 |
| ispartof | Digestive diseases and sciences, 2013-12, Vol.58 (12), p.3452-3459 |
| issn | 0163-2116 1573-2568 |
| language | eng |
| recordid | cdi_proquest_journals_1460838939 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acetylcysteine Acetylcysteine - administration & dosage Analysis Animals Antioxidants Biochemistry Colectomy - adverse effects Colitis Colitis - drug therapy Colitis - etiology Colitis - metabolism Colon - pathology DNA Damage - drug effects Enema Free Radical Scavengers - administration & dosage Gastroenterology Hepatology Inflammation Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Medicine Medicine & Public Health Oncology Original Article Oxidative Stress - drug effects Pouchitis - drug therapy Random Allocation Rats Rats, Wistar Transplant Surgery |
| title | Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T00%3A49%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enemas%20with%20n-Acetylcysteine%20Can%20Reduce%20the%20Level%20of%20Oxidative%20Damage%20in%20Cells%20of%20the%20Colonic%20Mucosa%20Diverted%20from%20the%20Faecal%20Stream&rft.jtitle=Digestive%20diseases%20and%20sciences&rft.au=Martinez,%20Carlos%20Augusto%20Real&rft.date=2013-12-01&rft.volume=58&rft.issue=12&rft.spage=3452&rft.epage=3459&rft.pages=3452-3459&rft.issn=0163-2116&rft.eissn=1573-2568&rft.coden=DDSCDJ&rft_id=info:doi/10.1007/s10620-013-2768-9&rft_dat=%3Cgale_proqu%3EA712943063%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1460838939&rft_id=info:pmid/23828143&rft_galeid=A712943063&rfr_iscdi=true |