Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate

Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate

In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in t...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Chemical & pharmaceutical bulletin 1976/07/25, Vol.24(7), pp.1510-1513
Hauptverfasser: NAMBARA, TOSHIO, NUMAZAWA, MITSUTERU, TSUCHIDA, YUKIKO, TANAKA, TOUICHI
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1513
container_issue 7
container_start_page 1510
container_title Chemical & pharmaceutical bulletin
container_volume 24
creator NAMBARA, TOSHIO
NUMAZAWA, MITSUTERU
TSUCHIDA, YUKIKO
TANAKA, TOUICHI
description In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that highly specific antiserum to estetrol would be produced by antigen whose steroidal moiety is coupled to a protein through the position remote from the inherent functional groups.
doi_str_mv 10.1248/cpb.24.1510
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1459817453</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3131789761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3710-2c7db9a6da5136eb1fca64495ffcfae45dc6f4be4d9b643c0a71620ac5d5ab453</originalsourceid><addsrcrecordid>eNpF0FtLwzAYBuAgCs7DlX8g4KV05tiul3POAwi7mF6Hr2kyM9pmJqm4f2_rRG_yBb4nb-BF6IqSKWVidqt31ZSJKZWUHKEJ5aLIJGP8GE0IIWXGeM5P0VmMW0KYJAWfoGa9M9pZp13aY2_xvEsumtC3OMBwqTFswHUx4WVMJgXf4D66boPzbJUtIFT-a9-a9L5v_JdrTXbnP11n8PonYd5Ufes6vPDdtt9AMhfoxEITzeXvPEdvD8vXxVP2snp8XsxfMs0LSjKmi7oqIa9BUp6biloNuRCltFZbMELWOreiMqIuq1xwTaCgOSOgZS2hEpKfo-tD7i74j97EpLa-D93wpaJCljNaDGhQNwelg48xGKt2wbUQ9ooSNdaphjoVE2qsc9D3B72NCTbmz0JITjdmtLSUs9EXh2N89r9-h6BMx78BCKiCXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1459817453</pqid></control><display><type>article</type><title>Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate</title><source>J-STAGE Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>NAMBARA, TOSHIO ; NUMAZAWA, MITSUTERU ; TSUCHIDA, YUKIKO ; TANAKA, TOUICHI</creator><creatorcontrib>NAMBARA, TOSHIO ; NUMAZAWA, MITSUTERU ; TSUCHIDA, YUKIKO ; TANAKA, TOUICHI</creatorcontrib><description>In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that highly specific antiserum to estetrol would be produced by antigen whose steroidal moiety is coupled to a protein through the position remote from the inherent functional groups.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.24.1510</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><ispartof>Chemical and Pharmaceutical Bulletin, 1976/07/25, Vol.24(7), pp.1510-1513</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 1976</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3710-2c7db9a6da5136eb1fca64495ffcfae45dc6f4be4d9b643c0a71620ac5d5ab453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>NAMBARA, TOSHIO</creatorcontrib><creatorcontrib>NUMAZAWA, MITSUTERU</creatorcontrib><creatorcontrib>TSUCHIDA, YUKIKO</creatorcontrib><creatorcontrib>TANAKA, TOUICHI</creatorcontrib><title>Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate</title><title>Chemical &amp; pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that highly specific antiserum to estetrol would be produced by antigen whose steroidal moiety is coupled to a protein through the position remote from the inherent functional groups.</description><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><recordid>eNpF0FtLwzAYBuAgCs7DlX8g4KV05tiul3POAwi7mF6Hr2kyM9pmJqm4f2_rRG_yBb4nb-BF6IqSKWVidqt31ZSJKZWUHKEJ5aLIJGP8GE0IIWXGeM5P0VmMW0KYJAWfoGa9M9pZp13aY2_xvEsumtC3OMBwqTFswHUx4WVMJgXf4D66boPzbJUtIFT-a9-a9L5v_JdrTXbnP11n8PonYd5Ufes6vPDdtt9AMhfoxEITzeXvPEdvD8vXxVP2snp8XsxfMs0LSjKmi7oqIa9BUp6biloNuRCltFZbMELWOreiMqIuq1xwTaCgOSOgZS2hEpKfo-tD7i74j97EpLa-D93wpaJCljNaDGhQNwelg48xGKt2wbUQ9ooSNdaphjoVE2qsc9D3B72NCTbmz0JITjdmtLSUs9EXh2N89r9-h6BMx78BCKiCXA</recordid><startdate>1976</startdate><enddate>1976</enddate><creator>NAMBARA, TOSHIO</creator><creator>NUMAZAWA, MITSUTERU</creator><creator>TSUCHIDA, YUKIKO</creator><creator>TANAKA, TOUICHI</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>1976</creationdate><title>Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate</title><author>NAMBARA, TOSHIO ; NUMAZAWA, MITSUTERU ; TSUCHIDA, YUKIKO ; TANAKA, TOUICHI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3710-2c7db9a6da5136eb1fca64495ffcfae45dc6f4be4d9b643c0a71620ac5d5ab453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAMBARA, TOSHIO</creatorcontrib><creatorcontrib>NUMAZAWA, MITSUTERU</creatorcontrib><creatorcontrib>TSUCHIDA, YUKIKO</creatorcontrib><creatorcontrib>TANAKA, TOUICHI</creatorcontrib><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Chemical &amp; pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAMBARA, TOSHIO</au><au>NUMAZAWA, MITSUTERU</au><au>TSUCHIDA, YUKIKO</au><au>TANAKA, TOUICHI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate</atitle><jtitle>Chemical &amp; pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1976</date><risdate>1976</risdate><volume>24</volume><issue>7</issue><spage>1510</spage><epage>1513</epage><pages>1510-1513</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><abstract>In order to obtain the specific antiserum used for radioimmunoassay of estetrol a new hapten-carrier conjugate was prepared from 6-ketoestetrol O-carboxymethyloxime by coupling with bovine serum albumin employing the mixed anhydride technique. The specificity of anti-estetrol antiserum elicited in the rabbit by immunization with this antigen was tested by cross-reaction studies with the closely related steroids. The results indicated that highly specific antiserum to estetrol would be produced by antigen whose steroidal moiety is coupled to a protein through the position remote from the inherent functional groups.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><doi>10.1248/cpb.24.1510</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0009-2363
ispartof Chemical and Pharmaceutical Bulletin, 1976/07/25, Vol.24(7), pp.1510-1513
issn 0009-2363
1347-5223
language eng
recordid cdi_proquest_journals_1459817453
source J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry
title Specificity of Antiserum raised against Estetrol using 6-O-Carboxymethyloxime-Bovine Serum Albumin Conjugate
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T11%3A13%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specificity%20of%20Antiserum%20raised%20against%20Estetrol%20using%206-O-Carboxymethyloxime-Bovine%20Serum%20Albumin%20Conjugate&rft.jtitle=Chemical%20&%20pharmaceutical%20bulletin&rft.au=NAMBARA,%20TOSHIO&rft.date=1976&rft.volume=24&rft.issue=7&rft.spage=1510&rft.epage=1513&rft.pages=1510-1513&rft.issn=0009-2363&rft.eissn=1347-5223&rft_id=info:doi/10.1248/cpb.24.1510&rft_dat=%3Cproquest_cross%3E3131789761%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1459817453&rft_id=info:pmid/&rfr_iscdi=true