Chemical Characterization of Seed Proteins of Mirabilis jalapa L. (Nyctaginaceae)

This article investigated chemical characteristics of Mirabilis jalapa seed proteins. Proteins were extracted from the deoiled seeds of Mirabilis jalapa L. in aqueous solutions at various pH values and also by using different concentrations of NaCl, Na ₂SO ₄, CaCl ₂, and MgSO ₄ at pH 7.0. The nitrog...

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Veröffentlicht in:International journal of food properties 2014-03, Vol.17 (3), p.559-569
Hauptverfasser: Ghosh, Asima, Nayak, Anupam, Banerji, Julie
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Banerji, Julie
description This article investigated chemical characteristics of Mirabilis jalapa seed proteins. Proteins were extracted from the deoiled seeds of Mirabilis jalapa L. in aqueous solutions at various pH values and also by using different concentrations of NaCl, Na ₂SO ₄, CaCl ₂, and MgSO ₄ at pH 7.0. The nitrogen content (1.77 ± 0.23 g/100 seed) of the seeds and deoiled seeds showed high protein content (11.0 ± 0.75 g/100 seed). Amino acid analyses of the total protein isolates confirmed the presence of 17 amino acids of which 9 are essential. The molecular weight of total protein isolates was determined by gel filtration and sodium dodecyl sulphate-poly acrylamide gel electrophoresis method. Studies on surface structure of the total protein isolates and seed flour by scanning electron microscopy were also performed.
doi_str_mv 10.1080/10942912.2011.642632
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subjects acrylamides
Amino acid
Amino acids
Aqueous solutions
flour
food quality
gel chromatography
gel electrophoresis
Gel filtration
Mirabilis jalapa
Molecular weight
nitrogen content
protein content
protein isolates
Proteins
Scanning electron microscopy
SDS-PAGE
Seed protein
Seeds
SEM
sodium
sodium chloride
title Chemical Characterization of Seed Proteins of Mirabilis jalapa L. (Nyctaginaceae)
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