A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38
Arthrofactin is a biosurfactant produced by Pseudomonas sp. MIS38. We have reported that transposon insertion into spoT (spoT::Tn5) causes moderate accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp) and abrogates arthrofactin production. To analyze the linkage of SpoT function and ablation of...
Gespeichert in:
| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011, Vol.75 (10), p.1880-1888 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1888 |
|---|---|
| container_issue | 10 |
| container_start_page | 1880 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 75 |
| creator | WASHIO, Kenji LIM, Siew Ping ROONGSAWANG, Niran MORIKAWA, Masaaki |
| description | Arthrofactin is a biosurfactant produced by Pseudomonas sp. MIS38. We have reported that transposon insertion into spoT (spoT::Tn5) causes moderate accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp) and abrogates arthrofactin production. To analyze the linkage of SpoT function and ablation of arthrofactin production, we examined the spoT::Tn5 mutation. The results showed that spoT::Tn5 is not a null mutation, but encodes separate segments of SpoT. Deletion of the 3′ region of spoT increased the level of arthrofactin production, suggesting that the C-terminal region of SpoT plays a suppressive role. We evaluated the expression of a distinct segment of SpoT. Forced expression of the C-terminal region that contains the ACT domain resulted in the accumulation of ppGpp and abrogated arthrofactin production. Expression of the C-terminal segment also reduced MIS38 swarming and resulted in extensive biofilm formation, which constitutes the phenocopy of the spoT::Tn5 mutant. |
| doi_str_mv | 10.1271/bbb.110042 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1449849299</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>900638129</sourcerecordid><originalsourceid>FETCH-LOGICAL-c344t-e1860be27ec5d9e25062c0c1cfab666015b6489fffd0e0dfed3a5166c88f71cd3</originalsourceid><addsrcrecordid>eNqNkt-K1DAUxoso7rh64wNIQESR7ZjTtGl7OY6768AsLux4XdL8sVnapJu0u8ydz-QTiU9iujOjICLeJCTn930nh3xR9BzwHJIc3tV1PQfAOE0eRDMgaR7TMs0fRjNcAo2LNIOj6In31xiHiwweR0cJlHmJKZlF3xdo40bD2SAFOrOuQ1ahq95uTtDK8HYU2nxBQyPRYrlBH2zHtJkqja714O8Ll86KkQ_amkm63PJWc7TWve1lP2gRlG5onFUsMEHLjEArjxbeW67vu97poUEXVkgXjui0lbfs4HY-MmO9NhKRH1-_nWRhid9r32-d7Rvr-2ZSrOWtbJE26NLLUdjOGuaR7-foYnVFiqfRI8VaL5_t9-Po89npZvkxXn86Xy0X65iTNB1iCQXFtUxyyTNRyiTDNOGYA1esppRiyGqaFqVSSmCJhZKCsAwo5UWhcuCCHEevd769szej9EPVac9l2zIj7eirEudQZAmB_yDDzxSQlIF8808SchLYHIAE9OUf6LUdnQkTV5CmZZGWSTkZvt1R3FnvnVRV73TH3LYCXE1ZqkKWql2WAvxibznWnRS_0EN4AvBqDzDPWascM1z731xGijDI1DXbcdqokDB2Z10rqoFtW-sOIvKXB_wEUSPmPg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449849299</pqid></control><display><type>article</type><title>A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Open Access Titles of Japan</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>WASHIO, Kenji ; LIM, Siew Ping ; ROONGSAWANG, Niran ; MORIKAWA, Masaaki</creator><creatorcontrib>WASHIO, Kenji ; LIM, Siew Ping ; ROONGSAWANG, Niran ; MORIKAWA, Masaaki</creatorcontrib><description>Arthrofactin is a biosurfactant produced by Pseudomonas sp. MIS38. We have reported that transposon insertion into spoT (spoT::Tn5) causes moderate accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp) and abrogates arthrofactin production. To analyze the linkage of SpoT function and ablation of arthrofactin production, we examined the spoT::Tn5 mutation. The results showed that spoT::Tn5 is not a null mutation, but encodes separate segments of SpoT. Deletion of the 3′ region of spoT increased the level of arthrofactin production, suggesting that the C-terminal region of SpoT plays a suppressive role. We evaluated the expression of a distinct segment of SpoT. Forced expression of the C-terminal region that contains the ACT domain resulted in the accumulation of ppGpp and abrogated arthrofactin production. Expression of the C-terminal segment also reduced MIS38 swarming and resulted in extensive biofilm formation, which constitutes the phenocopy of the spoT::Tn5 mutant.</description><identifier>ISSN: 0916-8451</identifier><identifier>ISSN: 1347-6947</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.110042</identifier><identifier>PMID: 21979063</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>(p)ppGpp ; ACT domain ; Amino Acid Sequence ; arthrofactin ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Deletion ; DNA Transposable Elements - genetics ; Elevation ; Fundamental and applied biological sciences. Psychology ; Genetic Loci - genetics ; Guanosine Tetraphosphate - metabolism ; Guanosines ; Lipopeptides - biosynthesis ; Molecular Sequence Data ; Mutations ; nonribosomal peptide synthetase ; Peptides, Cyclic - biosynthesis ; Protein Structure, Tertiary ; Pseudomonas ; Pseudomonas - enzymology ; Pseudomonas - genetics ; Pseudomonas - metabolism ; Pyrophosphatases - chemistry ; Pyrophosphatases - genetics ; Pyrophosphatases - metabolism ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Segments ; Sequence Deletion ; spoT ; Swarming ; Transcription, Genetic - genetics ; Transformation, Genetic</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2011, Vol.75 (10), p.1880-1888</ispartof><rights>2011 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c344t-e1860be27ec5d9e25062c0c1cfab666015b6489fffd0e0dfed3a5166c88f71cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25386389$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21979063$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WASHIO, Kenji</creatorcontrib><creatorcontrib>LIM, Siew Ping</creatorcontrib><creatorcontrib>ROONGSAWANG, Niran</creatorcontrib><creatorcontrib>MORIKAWA, Masaaki</creatorcontrib><title>A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Arthrofactin is a biosurfactant produced by Pseudomonas sp. MIS38. We have reported that transposon insertion into spoT (spoT::Tn5) causes moderate accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp) and abrogates arthrofactin production. To analyze the linkage of SpoT function and ablation of arthrofactin production, we examined the spoT::Tn5 mutation. The results showed that spoT::Tn5 is not a null mutation, but encodes separate segments of SpoT. Deletion of the 3′ region of spoT increased the level of arthrofactin production, suggesting that the C-terminal region of SpoT plays a suppressive role. We evaluated the expression of a distinct segment of SpoT. Forced expression of the C-terminal region that contains the ACT domain resulted in the accumulation of ppGpp and abrogated arthrofactin production. Expression of the C-terminal segment also reduced MIS38 swarming and resulted in extensive biofilm formation, which constitutes the phenocopy of the spoT::Tn5 mutant.</description><subject>(p)ppGpp</subject><subject>ACT domain</subject><subject>Amino Acid Sequence</subject><subject>arthrofactin</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Deletion</subject><subject>DNA Transposable Elements - genetics</subject><subject>Elevation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Loci - genetics</subject><subject>Guanosine Tetraphosphate - metabolism</subject><subject>Guanosines</subject><subject>Lipopeptides - biosynthesis</subject><subject>Molecular Sequence Data</subject><subject>Mutations</subject><subject>nonribosomal peptide synthetase</subject><subject>Peptides, Cyclic - biosynthesis</subject><subject>Protein Structure, Tertiary</subject><subject>Pseudomonas</subject><subject>Pseudomonas - enzymology</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - metabolism</subject><subject>Pyrophosphatases - chemistry</subject><subject>Pyrophosphatases - genetics</subject><subject>Pyrophosphatases - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Segments</subject><subject>Sequence Deletion</subject><subject>spoT</subject><subject>Swarming</subject><subject>Transcription, Genetic - genetics</subject><subject>Transformation, Genetic</subject><issn>0916-8451</issn><issn>1347-6947</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkt-K1DAUxoso7rh64wNIQESR7ZjTtGl7OY6768AsLux4XdL8sVnapJu0u8ydz-QTiU9iujOjICLeJCTn930nh3xR9BzwHJIc3tV1PQfAOE0eRDMgaR7TMs0fRjNcAo2LNIOj6In31xiHiwweR0cJlHmJKZlF3xdo40bD2SAFOrOuQ1ahq95uTtDK8HYU2nxBQyPRYrlBH2zHtJkqja714O8Ll86KkQ_amkm63PJWc7TWve1lP2gRlG5onFUsMEHLjEArjxbeW67vu97poUEXVkgXjui0lbfs4HY-MmO9NhKRH1-_nWRhid9r32-d7Rvr-2ZSrOWtbJE26NLLUdjOGuaR7-foYnVFiqfRI8VaL5_t9-Po89npZvkxXn86Xy0X65iTNB1iCQXFtUxyyTNRyiTDNOGYA1esppRiyGqaFqVSSmCJhZKCsAwo5UWhcuCCHEevd769szej9EPVac9l2zIj7eirEudQZAmB_yDDzxSQlIF8808SchLYHIAE9OUf6LUdnQkTV5CmZZGWSTkZvt1R3FnvnVRV73TH3LYCXE1ZqkKWql2WAvxibznWnRS_0EN4AvBqDzDPWascM1z731xGijDI1DXbcdqokDB2Z10rqoFtW-sOIvKXB_wEUSPmPg</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>WASHIO, Kenji</creator><creator>LIM, Siew Ping</creator><creator>ROONGSAWANG, Niran</creator><creator>MORIKAWA, Masaaki</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>C1K</scope><scope>P64</scope></search><sort><creationdate>2011</creationdate><title>A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38</title><author>WASHIO, Kenji ; LIM, Siew Ping ; ROONGSAWANG, Niran ; MORIKAWA, Masaaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-e1860be27ec5d9e25062c0c1cfab666015b6489fffd0e0dfed3a5166c88f71cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>(p)ppGpp</topic><topic>ACT domain</topic><topic>Amino Acid Sequence</topic><topic>arthrofactin</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Deletion</topic><topic>DNA Transposable Elements - genetics</topic><topic>Elevation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Loci - genetics</topic><topic>Guanosine Tetraphosphate - metabolism</topic><topic>Guanosines</topic><topic>Lipopeptides - biosynthesis</topic><topic>Molecular Sequence Data</topic><topic>Mutations</topic><topic>nonribosomal peptide synthetase</topic><topic>Peptides, Cyclic - biosynthesis</topic><topic>Protein Structure, Tertiary</topic><topic>Pseudomonas</topic><topic>Pseudomonas - enzymology</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - metabolism</topic><topic>Pyrophosphatases - chemistry</topic><topic>Pyrophosphatases - genetics</topic><topic>Pyrophosphatases - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Segments</topic><topic>Sequence Deletion</topic><topic>spoT</topic><topic>Swarming</topic><topic>Transcription, Genetic - genetics</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WASHIO, Kenji</creatorcontrib><creatorcontrib>LIM, Siew Ping</creatorcontrib><creatorcontrib>ROONGSAWANG, Niran</creatorcontrib><creatorcontrib>MORIKAWA, Masaaki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WASHIO, Kenji</au><au>LIM, Siew Ping</au><au>ROONGSAWANG, Niran</au><au>MORIKAWA, Masaaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2011</date><risdate>2011</risdate><volume>75</volume><issue>10</issue><spage>1880</spage><epage>1888</epage><pages>1880-1888</pages><issn>0916-8451</issn><issn>1347-6947</issn><eissn>1347-6947</eissn><abstract>Arthrofactin is a biosurfactant produced by Pseudomonas sp. MIS38. We have reported that transposon insertion into spoT (spoT::Tn5) causes moderate accumulation of guanosine 3′,5′-bispyrophosphate (ppGpp) and abrogates arthrofactin production. To analyze the linkage of SpoT function and ablation of arthrofactin production, we examined the spoT::Tn5 mutation. The results showed that spoT::Tn5 is not a null mutation, but encodes separate segments of SpoT. Deletion of the 3′ region of spoT increased the level of arthrofactin production, suggesting that the C-terminal region of SpoT plays a suppressive role. We evaluated the expression of a distinct segment of SpoT. Forced expression of the C-terminal region that contains the ACT domain resulted in the accumulation of ppGpp and abrogated arthrofactin production. Expression of the C-terminal segment also reduced MIS38 swarming and resulted in extensive biofilm formation, which constitutes the phenocopy of the spoT::Tn5 mutant.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>21979063</pmid><doi>10.1271/bbb.110042</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2011, Vol.75 (10), p.1880-1888 |
| issn | 0916-8451 1347-6947 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449849299 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | (p)ppGpp ACT domain Amino Acid Sequence arthrofactin Base Sequence Biological and medical sciences Biotechnology Deletion DNA Transposable Elements - genetics Elevation Fundamental and applied biological sciences. Psychology Genetic Loci - genetics Guanosine Tetraphosphate - metabolism Guanosines Lipopeptides - biosynthesis Molecular Sequence Data Mutations nonribosomal peptide synthetase Peptides, Cyclic - biosynthesis Protein Structure, Tertiary Pseudomonas Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas - metabolism Pyrophosphatases - chemistry Pyrophosphatases - genetics Pyrophosphatases - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Segments Sequence Deletion spoT Swarming Transcription, Genetic - genetics Transformation, Genetic |
| title | A Truncated Form of SpoT, Including the ACT Domain, Inhibits the Production of Cyclic Lipopeptide Arthrofactin, and Is Associated with Moderate Elevation of Guanosine 3′,5′-Bispyrophosphate Level in Pseudomonas sp. MIS38 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T09%3A28%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20Truncated%20Form%20of%20SpoT,%20Including%20the%20ACT%20Domain,%20Inhibits%20the%20Production%20of%20Cyclic%20Lipopeptide%20Arthrofactin,%20and%20Is%20Associated%20with%20Moderate%20Elevation%20of%20Guanosine%203%E2%80%B2,5%E2%80%B2-Bispyrophosphate%20Level%20in%20Pseudomonas%20sp.%20MIS38&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=WASHIO,%20Kenji&rft.date=2011&rft.volume=75&rft.issue=10&rft.spage=1880&rft.epage=1888&rft.pages=1880-1888&rft.issn=0916-8451&rft.eissn=1347-6947&rft_id=info:doi/10.1271/bbb.110042&rft_dat=%3Cproquest_cross%3E900638129%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449849299&rft_id=info:pmid/21979063&rfr_iscdi=true |