Cloning and Expression of a [beta]-Galactosidase Gene of Bacillus circulans

Cloning and Expression of a [beta]-Galactosidase Gene of Bacillus circulans

A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2011-06, Vol.75 (6), p.1194
Hauptverfasser: SONG, Jingyuan, IMANAKA, Hiroyuki, IMAMURA, Koreyoshi, MINODA, Masashi, KATASE, Toru, HOSHI, Yukiko, YAMAGUCHI, Shotaro, NAKANISHI, Kazuhiro
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container_issue 6
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container_title Bioscience, biotechnology, and biochemistry
container_volume 75
creator SONG, Jingyuan
IMANAKA, Hiroyuki
IMAMURA, Koreyoshi
MINODA, Masashi
KATASE, Toru
HOSHI, Yukiko
YAMAGUCHI, Shotaro
NAKANISHI, Kazuhiro
description A gene of β-galactosidase from Bacillus circulans ATCC 31382 was cloned and sequenced on the basis of N-terminal and internal peptide sequences isolated from a commercial enzyme preparation, Biolacta®. Using the cloned gene, recombinant β-galactosidase and its deletion mutants were overexpressed as His-tagged proteins in Escherichia coli cells and the enzymes expressed were characterized.
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source J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry
title Cloning and Expression of a [beta]-Galactosidase Gene of Bacillus circulans
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