Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster
Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for poly...
Gespeichert in:
| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1412-1415 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1415 |
|---|---|
| container_issue | 6 |
| container_start_page | 1412 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 73 |
| creator | JUNG, Hye-Jin PARK, Sung-Hee HA, Sang-Do LEE, Kyu-Ho CHUNG, Duck Hwa KIM, Cheol-Ho KIM, Min-Gon KIM, Kwang-Yup KIM, Keun-Sung |
| description | Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species. |
| doi_str_mv | 10.1271/bbb.70252 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1449842337</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20165575</sourcerecordid><originalsourceid>FETCH-LOGICAL-c544t-a1883fab8e07cbf02a31cc0bc083747df424ae1d76a8d325816889ba931750d93</originalsourceid><addsrcrecordid>eNqF0d-K1DAUBvAgijuuXvgCEhAFL7rm5E-TXg6jrsKAorveljQ9mc3SNmPSIn0A39vOzqigF14dCL_zHcJHyFNgF8A1vG6a5kIzrvg9sgIhdVFWUt8nK1ZBWRip4Iw8yvmWseVBwUNyBpViXJdsRX582aMLmIu76YOjb3BEN4Y40OjpNuQRU7C0j0N08xh3OGCm1zkMO_opdnOPyWakmxsbBvoZ7XFznbOdM72yaYfjgY43SPfJr-nXkKYOB4f0ckmim246HHhMHnjbZXxymufk-t3bq837Yvvx8sNmvS2cknIsLBgjvG0MMu0az7gV4BxrHDNCS916yaVFaHVpTSu4MlAaUzW2EqAVaytxTl4ec_cpfpswj3UfssOuswPGKdellgBg4L-QMyiV0mqBz_-Ct3FKw_KJGqSsjORC6EW9OiqXYs4Jfb1PobdproHVhwrrpcL6rsLFPjslTk2P7R956mwBL07AZmc7n-zgQv7tOOhKMmkWJ48uDD6m3n6PqWvr0c5dTL-WxL_3fwJNQLX8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449842337</pqid></control><display><type>article</type><title>Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Freely Accessible Japanese Titles</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>JUNG, Hye-Jin ; PARK, Sung-Hee ; HA, Sang-Do ; LEE, Kyu-Ho ; CHUNG, Duck Hwa ; KIM, Cheol-Ho ; KIM, Min-Gon ; KIM, Kwang-Yup ; KIM, Keun-Sung</creator><creatorcontrib>JUNG, Hye-Jin ; PARK, Sung-Hee ; HA, Sang-Do ; LEE, Kyu-Ho ; CHUNG, Duck Hwa ; KIM, Cheol-Ho ; KIM, Min-Gon ; KIM, Kwang-Yup ; KIM, Keun-Sung</creatorcontrib><description>Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.70252</identifier><identifier>PMID: 19502760</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA Primers ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Listeria monocytogenes ; Listeria monocytogenes - genetics ; Listeria monocytogenes - pathogenicity ; Multigene Family ; PCR ; Polymerase Chain Reaction - methods ; prfA virulence gene cluster ; Species Specificity ; Virulence - genetics</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1412-1415</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c544t-a1883fab8e07cbf02a31cc0bc083747df424ae1d76a8d325816889ba931750d93</citedby><cites>FETCH-LOGICAL-c544t-a1883fab8e07cbf02a31cc0bc083747df424ae1d76a8d325816889ba931750d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21794048$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19502760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JUNG, Hye-Jin</creatorcontrib><creatorcontrib>PARK, Sung-Hee</creatorcontrib><creatorcontrib>HA, Sang-Do</creatorcontrib><creatorcontrib>LEE, Kyu-Ho</creatorcontrib><creatorcontrib>CHUNG, Duck Hwa</creatorcontrib><creatorcontrib>KIM, Cheol-Ho</creatorcontrib><creatorcontrib>KIM, Min-Gon</creatorcontrib><creatorcontrib>KIM, Kwang-Yup</creatorcontrib><creatorcontrib>KIM, Keun-Sung</creatorcontrib><title>Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Primers</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - genetics</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Multigene Family</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>prfA virulence gene cluster</subject><subject>Species Specificity</subject><subject>Virulence - genetics</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0d-K1DAUBvAgijuuXvgCEhAFL7rm5E-TXg6jrsKAorveljQ9mc3SNmPSIn0A39vOzqigF14dCL_zHcJHyFNgF8A1vG6a5kIzrvg9sgIhdVFWUt8nK1ZBWRip4Iw8yvmWseVBwUNyBpViXJdsRX582aMLmIu76YOjb3BEN4Y40OjpNuQRU7C0j0N08xh3OGCm1zkMO_opdnOPyWakmxsbBvoZ7XFznbOdM72yaYfjgY43SPfJr-nXkKYOB4f0ckmim246HHhMHnjbZXxymufk-t3bq837Yvvx8sNmvS2cknIsLBgjvG0MMu0az7gV4BxrHDNCS916yaVFaHVpTSu4MlAaUzW2EqAVaytxTl4ec_cpfpswj3UfssOuswPGKdellgBg4L-QMyiV0mqBz_-Ct3FKw_KJGqSsjORC6EW9OiqXYs4Jfb1PobdproHVhwrrpcL6rsLFPjslTk2P7R956mwBL07AZmc7n-zgQv7tOOhKMmkWJ48uDD6m3n6PqWvr0c5dTL-WxL_3fwJNQLX8</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>JUNG, Hye-Jin</creator><creator>PARK, Sung-Hee</creator><creator>HA, Sang-Do</creator><creator>LEE, Kyu-Ho</creator><creator>CHUNG, Duck Hwa</creator><creator>KIM, Cheol-Ho</creator><creator>KIM, Min-Gon</creator><creator>KIM, Kwang-Yup</creator><creator>KIM, Keun-Sung</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster</title><author>JUNG, Hye-Jin ; PARK, Sung-Hee ; HA, Sang-Do ; LEE, Kyu-Ho ; CHUNG, Duck Hwa ; KIM, Cheol-Ho ; KIM, Min-Gon ; KIM, Kwang-Yup ; KIM, Keun-Sung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c544t-a1883fab8e07cbf02a31cc0bc083747df424ae1d76a8d325816889ba931750d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Primers</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - genetics</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Multigene Family</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>prfA virulence gene cluster</topic><topic>Species Specificity</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JUNG, Hye-Jin</creatorcontrib><creatorcontrib>PARK, Sung-Hee</creatorcontrib><creatorcontrib>HA, Sang-Do</creatorcontrib><creatorcontrib>LEE, Kyu-Ho</creatorcontrib><creatorcontrib>CHUNG, Duck Hwa</creatorcontrib><creatorcontrib>KIM, Cheol-Ho</creatorcontrib><creatorcontrib>KIM, Min-Gon</creatorcontrib><creatorcontrib>KIM, Kwang-Yup</creatorcontrib><creatorcontrib>KIM, Keun-Sung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JUNG, Hye-Jin</au><au>PARK, Sung-Hee</au><au>HA, Sang-Do</au><au>LEE, Kyu-Ho</au><au>CHUNG, Duck Hwa</au><au>KIM, Cheol-Ho</au><au>KIM, Min-Gon</au><au>KIM, Kwang-Yup</au><au>KIM, Keun-Sung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>73</volume><issue>6</issue><spage>1412</spage><epage>1415</epage><pages>1412-1415</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Sequence comparison of individual genes in the prfA virulence gene cluster (pVGC), a central virulence gene cluster, from different Listeria species indicated that priming sites within the genes appeared to be specific for Listeria monocytogenes exclusively. Therefore, the pVGC was targeted for polymerase chain reaction (PCR) assays to detect and specifically identify L. monocytogenes. Each gene of the pVGC was specifically amplified in L. monocytogenes but not in other Listeria species.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>19502760</pmid><doi>10.1271/bbb.70252</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2009-06, Vol.73 (6), p.1412-1415 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449842337 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Biological and medical sciences DNA Primers Fundamental and applied biological sciences. Psychology Genes, Bacterial Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - pathogenicity Multigene Family PCR Polymerase Chain Reaction - methods prfA virulence gene cluster Species Specificity Virulence - genetics |
| title | Species-Specific Detection of Listeria monocytogenes Using Polymerase Chain Reaction Assays Targeting the prfA Virulence Gene Cluster |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T06%3A20%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Species-Specific%20Detection%20of%20Listeria%20monocytogenes%20Using%20Polymerase%20Chain%20Reaction%20Assays%20Targeting%20the%20prfA%20Virulence%20Gene%20Cluster&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=JUNG,%20Hye-Jin&rft.date=2009-06-01&rft.volume=73&rft.issue=6&rft.spage=1412&rft.epage=1415&rft.pages=1412-1415&rft.issn=0916-8451&rft.eissn=1347-6947&rft_id=info:doi/10.1271/bbb.70252&rft_dat=%3Cproquest_cross%3E20165575%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449842337&rft_id=info:pmid/19502760&rfr_iscdi=true |