Inducer-dependent nuclear localization of a Zn(II)2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans
AmyR is a Zn(II) 2 Cys 6 transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physi...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2009-02, Vol.73 (2), p.391-399 |
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| container_end_page | 399 |
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| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 73 |
| creator | Makita, T.(Nagoya Univ. (Japan)) Katsuyama, Y Tani, S Suzuki, H Kato, N Todd, R.B Hynes, M.J Tsukagoshi, N Kato, M Kobayashi, T |
| description | AmyR is a Zn(II)
2
Cys
6
transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn(II)
2
Cys
6
DNA binding motif. |
| doi_str_mv | 10.1271/bbb.80654 |
| format | Article |
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2
Cys
6
transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn(II)
2
Cys
6
DNA binding motif.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.80654</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ACTIVADOR GENICO ; ACTIVATEUR GENIQUE ; AMILASAS ; AMYLASE ; AMYLASES ; amyR ; Aspergillus ; ASPERGILLUS NIDULANS ; Biological and medical sciences ; EXPRESION GENICA ; EXPRESSION DES GENES ; FACTEUR DE TRANSCRIPTION ; FACTORES DE TRANSCRIPCION ; Fundamental and applied biological sciences. Psychology ; GENE ACTIVATORS ; GENE EXPRESSION ; induction ; nuclear localization ; TRANSCRIPTION FACTORS</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2009-02, Vol.73 (2), p.391-399</ispartof><rights>2009 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21325044$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Makita, T.(Nagoya Univ. (Japan))</creatorcontrib><creatorcontrib>Katsuyama, Y</creatorcontrib><creatorcontrib>Tani, S</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Kato, N</creatorcontrib><creatorcontrib>Todd, R.B</creatorcontrib><creatorcontrib>Hynes, M.J</creatorcontrib><creatorcontrib>Tsukagoshi, N</creatorcontrib><creatorcontrib>Kato, M</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><title>Inducer-dependent nuclear localization of a Zn(II)2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans</title><title>Bioscience, biotechnology, and biochemistry</title><description>AmyR is a Zn(II)
2
Cys
6
transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn(II)
2
Cys
6
DNA binding motif.</description><subject>ACTIVADOR GENICO</subject><subject>ACTIVATEUR GENIQUE</subject><subject>AMILASAS</subject><subject>AMYLASE</subject><subject>AMYLASES</subject><subject>amyR</subject><subject>Aspergillus</subject><subject>ASPERGILLUS NIDULANS</subject><subject>Biological and medical sciences</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FACTEUR DE TRANSCRIPTION</subject><subject>FACTORES DE TRANSCRIPCION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE ACTIVATORS</subject><subject>GENE EXPRESSION</subject><subject>induction</subject><subject>nuclear localization</subject><subject>TRANSCRIPTION FACTORS</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNo1kVuLFDEQhYMoOO764A8QAiIobK9JOp1OHofByywLu4i--BKqc1myZJI26VbGX2_WWZ8Kqr5T1KmD0CtKLikb6Ydpmi4lEQN_gja052MnFB-fog1RVHSSD_Q5elHrPSGtMdANmvfJrsaVzrrZJevSgtNqooOCYzYQwx9YQk44ewz4R3q3379nu2MVeCmQqilhfhhDxGCW8AuWXC7w9nD8eoFDwts6u3IXYlwrTsGusUnO0TMPsbqXj_UMff_08dvuS3d983m_2153vudi6aySjMLgSG-UotZLLziMXFEnPOFmMtQID16N0kwM1Ah28JOUzMvRc2fH_gy9Oe2dS_65urro-7yWdmnVlHMlOeWsb9TbRwpqc-ubKROqnks4QDlqRns2EM4bx09cSD6XA_zOJVq9wDHm8l_UU6IfMtAtA_0vgyZ7fZJ5yBruSqOubln7PSGUDKL_C9eng6g</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Makita, T.(Nagoya Univ. (Japan))</creator><creator>Katsuyama, Y</creator><creator>Tani, S</creator><creator>Suzuki, H</creator><creator>Kato, N</creator><creator>Todd, R.B</creator><creator>Hynes, M.J</creator><creator>Tsukagoshi, N</creator><creator>Kato, M</creator><creator>Kobayashi, T</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>20090201</creationdate><title>Inducer-dependent nuclear localization of a Zn(II)2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans</title><author>Makita, T.(Nagoya Univ. (Japan)) ; Katsuyama, Y ; Tani, S ; Suzuki, H ; Kato, N ; Todd, R.B ; Hynes, M.J ; Tsukagoshi, N ; Kato, M ; Kobayashi, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f346t-d9821a5e03c991df8f64a7491e6f04cbc1c6faf978cb2a97ad5fb882f87f4ed73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ACTIVADOR GENICO</topic><topic>ACTIVATEUR GENIQUE</topic><topic>AMILASAS</topic><topic>AMYLASE</topic><topic>AMYLASES</topic><topic>amyR</topic><topic>Aspergillus</topic><topic>ASPERGILLUS NIDULANS</topic><topic>Biological and medical sciences</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FACTEUR DE TRANSCRIPTION</topic><topic>FACTORES DE TRANSCRIPCION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE ACTIVATORS</topic><topic>GENE EXPRESSION</topic><topic>induction</topic><topic>nuclear localization</topic><topic>TRANSCRIPTION FACTORS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Makita, T.(Nagoya Univ. (Japan))</creatorcontrib><creatorcontrib>Katsuyama, Y</creatorcontrib><creatorcontrib>Tani, S</creatorcontrib><creatorcontrib>Suzuki, H</creatorcontrib><creatorcontrib>Kato, N</creatorcontrib><creatorcontrib>Todd, R.B</creatorcontrib><creatorcontrib>Hynes, M.J</creatorcontrib><creatorcontrib>Tsukagoshi, N</creatorcontrib><creatorcontrib>Kato, M</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Makita, T.(Nagoya Univ. (Japan))</au><au>Katsuyama, Y</au><au>Tani, S</au><au>Suzuki, H</au><au>Kato, N</au><au>Todd, R.B</au><au>Hynes, M.J</au><au>Tsukagoshi, N</au><au>Kato, M</au><au>Kobayashi, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inducer-dependent nuclear localization of a Zn(II)2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><date>2009-02-01</date><risdate>2009</risdate><volume>73</volume><issue>2</issue><spage>391</spage><epage>399</epage><pages>391-399</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>AmyR is a Zn(II)
2
Cys
6
transcriptional activator that regulates expression of the amylolytic genes in Aspergillus species. Subcellular localization studies of GFP-fused AmyR in A. nidulans revealed that the fusion protein preferentially localized to the nucleus in response to isomaltose, the physiological inducer of the amylolytic genes. The C-terminal domains of AmyR, designated MH3 (residues 419-496) and MH4 (residues 516-542), were essential for sensing the inducing stimulus and regulating the subcellular localization. The MH2 domain (residues 234-375) located in the middle of AmyR was required for transcriptional activation of the target genes, and the nuclear localization signals were identified within the N-terminal Zn(II)
2
Cys
6
DNA binding motif.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><doi>10.1271/bbb.80654</doi><tpages>9</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2009-02, Vol.73 (2), p.391-399 |
| issn | 0916-8451 1347-6947 |
| language | eng |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | ACTIVADOR GENICO ACTIVATEUR GENIQUE AMILASAS AMYLASE AMYLASES amyR Aspergillus ASPERGILLUS NIDULANS Biological and medical sciences EXPRESION GENICA EXPRESSION DES GENES FACTEUR DE TRANSCRIPTION FACTORES DE TRANSCRIPCION Fundamental and applied biological sciences. Psychology GENE ACTIVATORS GENE EXPRESSION induction nuclear localization TRANSCRIPTION FACTORS |
| title | Inducer-dependent nuclear localization of a Zn(II)2Cys6 transcriptional activator, AmyR, in Aspergillus nidulans |
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