Molecular Cloning of cDNAs and Genes for Three [alpha]-Glucosidases from European Honeybees, Apis mellifera L., and Heterologous Production of Recombinant Enzymes in Pichia pastoris

cDNAs encoding three α-glucosidases (HBGases I, II, and III) from European honeybees, Apis mellifera, were cloned and sequenced, two of which were expressed in Pichia pastoris. The cDNAs for HBGases I, II, and III were 1,986, 1,910, and 1,915 bp in length, and included ORFs of 1,767, 1,743, and 1,70...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2007-07, Vol.71 (7), p.1703
Hauptverfasser: NISHIMOTO, Mamoru, MORI, Haruhide, MOTEKI, Tsuneharu, TAKAMURA, Yukiko, IWAI, Gaku, MIYAGUCHI, Yu, OKUYAMA, Masayuki, WONGCHAWALIT, Jintanart, SURARIT, Rudee, SVASTI, Jisnuson, KIMURA, Atsuo, CHIBA, Seiya
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Sprache:eng
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