Hydroxylation of oleanolic acid to queretaroic acid by cytochrome P450 from Nonomuraea recticatena

A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produ...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-09, Vol.70 (9), p.2299-2302
Hauptverfasser:	Fujii, Y.(Mercian Corp., Iwata, Shizuoka (Japan). Bioresource Labs.), Hirosue, S, Fujii, T, Matsumoto, N, Agematu, H, Arisawa, A
Format:	Artikel
Sprache:	eng
Schlagworte:	ACIDE ORGANIQUE ACIDOS ORGANICOS Actinobacteria - enzymology Actinobacteria - genetics Actinobacteria - metabolism ACTINOMYCETALES ACTIVIDAD CATALITICA ACTIVITE CATALYTIQUE Biological and medical sciences CATALYTIC ACTIVITY Cell-Free System CITOCROMO P450 Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism CYTOCHROME P450 Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology HYDROXYLATION IDROXILACION INDUSTRIA FARMACEUTICA Industrial Microbiology - methods INDUSTRIE PHARMACEUTIQUE Molecular Structure Nuclear Magnetic Resonance, Biomolecular oleanolic acid Oleanolic Acid - metabolism ORGANIC ACIDS PHARMACEUTICAL INDUSTRY queretaroic acid Recombinant Proteins - genetics Recombinant Proteins - metabolism Spectrometry, Mass, Electrospray Ionization TRITERPENOIDE TRITERPENOIDOS TRITERPENOIDS
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Zusammenfassung:	A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.60126