N-Linked Glycan Structures of Human Lactoferrin Produced by Transgenic Rice

Human lactoferrin was produced in genetically engineered rice. N-linked glycan structures of recombinant human lactoferrin were determined. The oligosaccharides liberated by hydrazinolysis were labeled with 2-aminopyridine (PA). The PA-labeled glycans were purified by reverse-phase and size-fraction...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2004-12, Vol.68 (12), p.2565-2570
Hauptverfasser:	FUJIYAMA, Kazuhito, SAKAI, Yohei, MISAKI, Ryo, YANAGIHARA, Itaru, HONDA, Takeshi, ANZAI, Hiroyuki, SEKI, Tatsuji
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology glycosylation heterologous expression Humans lactoferrin Lactoferrin - biosynthesis Lactoferrin - chemistry Lactoferrin - genetics Oryza - genetics Oryza sativa Plants, Genetically Modified Polysaccharides - chemistry Recombinant Proteins - chemistry rice Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization structure
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