Isothermal titration calorimetric studies on the binding of a family 6 carbohydrate-binding module of Clostridium thermocellum XynA with xlylooligosaccharides

The family 6 carbohydrate-binding module (CBM) of Clostridium thermocellum XynA was expressed, and the binding equilibria of the CBM with xylooligosaccharides (degree of polymerization DP=2−8) were observed by isothermal titration calorimetry (ITC) at pH 8. The association constant, K a , increased...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-02, Vol.67 (2), p.406-409
Hauptverfasser:	Sakka, K. (Mie Univ., Tsu (Japan). Faculty of Bioresources), Nakanishi, M, Sogabe, M, Arai, T, Ohara, H, Tanaka, A, Kimura, T, Ohmiya, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding Sites Biological and medical sciences CALORIMETRY Calorimetry - methods Carbohydrate Metabolism carbohydrate-binding module CARBOHYDRATES Carbohydrates - chemistry Clostridium - enzymology Clostridium - metabolism CLOSTRIDIUM THERMOCELLUM Endo-1,4-beta Xylanases Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration isothermal titration calorimetry Kinetics LIGANDS OLIGOSACCHARIDES Oligosaccharides - chemistry Oligosaccharides - metabolism Protein Binding THERMOCHEMICAL PROPERTIES Thermodynamics TITRIMETRY xylanase XYLOSE Xylosidases - biosynthesis Xylosidases - chemistry Xylosidases - metabolism
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