Structures and expression patterns of two tropinone reductase genes from Hyoscyamus niger
In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-10, Vol.63 (10), p.1756-1764 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Nakajima, K. (Nara Inst. of Science and Technology, Ikoma (Japan)) Oshita, Y Kaya, M Yamada, Y Hashimoto, T |
| description | In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same exon/intron organization, sequence similarity was restricted to the coding regions. In H. niger transgenic hairy roots, both TR promoters activated transcription of the reporter genes in endodermis and pericycle of the roots. A quantitative reporter assay and a nuclear run-on experiment indicated that the two genes are transcribed at a similar rate. The results indicate that although different activity levels have been observed for the TR enzymes in the H. niger root, the TR genes per se conserve similar tissue-specific expression pattern and transcriptional rate |
| doi_str_mv | 10.1271/bbb.63.1756 |
| format | Article |
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(Nara Inst. of Science and Technology, Ikoma (Japan)) ; Oshita, Y ; Kaya, M ; Yamada, Y ; Hashimoto, T</creator><creatorcontrib>Nakajima, K. (Nara Inst. of Science and Technology, Ikoma (Japan)) ; Oshita, Y ; Kaya, M ; Yamada, Y ; Hashimoto, T</creatorcontrib><description>In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same exon/intron organization, sequence similarity was restricted to the coding regions. In H. niger transgenic hairy roots, both TR promoters activated transcription of the reporter genes in endodermis and pericycle of the roots. A quantitative reporter assay and a nuclear run-on experiment indicated that the two genes are transcribed at a similar rate. The results indicate that although different activity levels have been observed for the TR enzymes in the H. niger root, the TR genes per se conserve similar tissue-specific expression pattern and transcriptional rate</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.63.1756</identifier><identifier>PMID: 10586504</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>ALCALOIDE ; ALCALOIDES ; Alcohol Oxidoreductases - genetics ; Alcohol Oxidoreductases - metabolism ; ALKALOIDS ; Artificial Gene Fusion ; Base Sequence ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; BIOSYNTHESIS ; CLONACION ; CLONAGE ; CLONING ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; Gene Expression Regulation, Plant ; Gene Library ; Genes, Plant ; Genes, Reporter - genetics ; Genes. Genome ; histochemical analysis ; HYOSCYAMUS NIGER ; Models, Chemical ; Models, Genetic ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; OXIDOREDUCTASES ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; Plants, Medicinal - enzymology ; Plants, Medicinal - genetics ; Promoter Regions, Genetic ; RACINE ; RAICES ; reporter gene ; ROOTS ; Sequence Homology, Nucleic Acid ; Solanaceae - enzymology ; Solanaceae - genetics ; Solanaceae - ultrastructure ; TATA Box - genetics ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription, Genetic ; tropane alkaloid ; tropinone reductase</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1999-10, Vol.63 (10), p.1756-1764</ispartof><rights>1999 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1999</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-91b2ef06d988e4d5fe3993b17072505d029419a04b0d46e5e6aaa4036fe1e1533</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1252600$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10586504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakajima, K. (Nara Inst. of Science and Technology, Ikoma (Japan))</creatorcontrib><creatorcontrib>Oshita, Y</creatorcontrib><creatorcontrib>Kaya, M</creatorcontrib><creatorcontrib>Yamada, Y</creatorcontrib><creatorcontrib>Hashimoto, T</creatorcontrib><title>Structures and expression patterns of two tropinone reductase genes from Hyoscyamus niger</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same exon/intron organization, sequence similarity was restricted to the coding regions. In H. niger transgenic hairy roots, both TR promoters activated transcription of the reporter genes in endodermis and pericycle of the roots. A quantitative reporter assay and a nuclear run-on experiment indicated that the two genes are transcribed at a similar rate. The results indicate that although different activity levels have been observed for the TR enzymes in the H. niger root, the TR genes per se conserve similar tissue-specific expression pattern and transcriptional rate</description><subject>ALCALOIDE</subject><subject>ALCALOIDES</subject><subject>Alcohol Oxidoreductases - genetics</subject><subject>Alcohol Oxidoreductases - metabolism</subject><subject>ALKALOIDS</subject><subject>Artificial Gene Fusion</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>BIOSYNTHESIS</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Library</subject><subject>Genes, Plant</subject><subject>Genes, Reporter - genetics</subject><subject>Genes. Genome</subject><subject>histochemical analysis</subject><subject>HYOSCYAMUS NIGER</subject><subject>Models, Chemical</subject><subject>Models, Genetic</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>OXIDOREDUCTASES</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>Plants, Medicinal - enzymology</subject><subject>Plants, Medicinal - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>RACINE</subject><subject>RAICES</subject><subject>reporter gene</subject><subject>ROOTS</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Solanaceae - enzymology</subject><subject>Solanaceae - genetics</subject><subject>Solanaceae - ultrastructure</subject><subject>TATA Box - genetics</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><subject>tropane alkaloid</subject><subject>tropinone reductase</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M2L1DAYBvAgijuOnjwrAcWLdMx3m-OyqOuyoKAePIW0fTN0aZOapKzz32-Gjqws5pIQfs_Ly4PQS0p2lNX0Q9u2O8V3tJbqEdpQLupKaVE_RhuiqaoaIekZepbSDSHlQ9Kn6IwS2ShJxAb9-p7j0uUlQsLW9xj-zOWZhuDxbHOG6BMODufbgHMM8-CDBxyhLxmbAO_Bl6CLYcKXh5C6g52WhP2wh_gcPXF2TPDidG_Rz08ff1xcVtdfP3-5OL-uOqF0rjRtGTiiet00IHrpgGvNW1qTmkkie8K0oNoS0ZJeKJCgrLWCcOWAApWcb9G7de4cw-8FUjbTkDoYR-shLMkozblQNSvwzQN4E5boy26GCqEFF02xW_R-VV0MKUVwZo7DZOPBUGKOfZvSt1HcHPsu-vVp5tJO0P9j14ILeHsCNnV2dNH6bkj3jkmmCClMrWzwLsTJ3oY49ibbwxji3wz__wKv1qCzwdh9LO7qGyPHwxqp-R3RaKMz</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Nakajima, K. 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(Nara Inst. of Science and Technology, Ikoma (Japan)) ; Oshita, Y ; Kaya, M ; Yamada, Y ; Hashimoto, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-91b2ef06d988e4d5fe3993b17072505d029419a04b0d46e5e6aaa4036fe1e1533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>ALCALOIDE</topic><topic>ALCALOIDES</topic><topic>Alcohol Oxidoreductases - genetics</topic><topic>Alcohol Oxidoreductases - metabolism</topic><topic>ALKALOIDS</topic><topic>Artificial Gene Fusion</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>BIOSYNTHESIS</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Library</topic><topic>Genes, Plant</topic><topic>Genes, Reporter - genetics</topic><topic>Genes. Genome</topic><topic>histochemical analysis</topic><topic>HYOSCYAMUS NIGER</topic><topic>Models, Chemical</topic><topic>Models, Genetic</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>Plants, Medicinal - enzymology</topic><topic>Plants, Medicinal - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>RACINE</topic><topic>RAICES</topic><topic>reporter gene</topic><topic>ROOTS</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Solanaceae - enzymology</topic><topic>Solanaceae - genetics</topic><topic>Solanaceae - ultrastructure</topic><topic>TATA Box - genetics</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic</topic><topic>tropane alkaloid</topic><topic>tropinone reductase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakajima, K. (Nara Inst. of Science and Technology, Ikoma (Japan))</creatorcontrib><creatorcontrib>Oshita, Y</creatorcontrib><creatorcontrib>Kaya, M</creatorcontrib><creatorcontrib>Yamada, Y</creatorcontrib><creatorcontrib>Hashimoto, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakajima, K. (Nara Inst. of Science and Technology, Ikoma (Japan))</au><au>Oshita, Y</au><au>Kaya, M</au><au>Yamada, Y</au><au>Hashimoto, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structures and expression patterns of two tropinone reductase genes from Hyoscyamus niger</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>63</volume><issue>10</issue><spage>1756</spage><epage>1764</epage><pages>1756-1764</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>In the biosynthesis of tropane alkaloids, two tropinone reductases (TRs) catalyze reduction of tropinone to different stereoisomers, tropine and pseudotropine. Two TRs from Hyoscyamus niger have 64% of identical amino acids and hence a common evolutionary origin. In this study, genomic clones of TRs were isolated from H. niger. Their sequence comparison showed that although they have the same exon/intron organization, sequence similarity was restricted to the coding regions. In H. niger transgenic hairy roots, both TR promoters activated transcription of the reporter genes in endodermis and pericycle of the roots. A quantitative reporter assay and a nuclear run-on experiment indicated that the two genes are transcribed at a similar rate. The results indicate that although different activity levels have been observed for the TR enzymes in the H. niger root, the TR genes per se conserve similar tissue-specific expression pattern and transcriptional rate</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>10586504</pmid><doi>10.1271/bbb.63.1756</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1999-10, Vol.63 (10), p.1756-1764 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449434869 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | ALCALOIDE ALCALOIDES Alcohol Oxidoreductases - genetics Alcohol Oxidoreductases - metabolism ALKALOIDS Artificial Gene Fusion Base Sequence Biological and medical sciences BIOSINTESIS BIOSYNTHESE BIOSYNTHESIS CLONACION CLONAGE CLONING EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Expression Regulation, Plant Gene Library Genes, Plant Genes, Reporter - genetics Genes. Genome histochemical analysis HYOSCYAMUS NIGER Models, Chemical Models, Genetic Molecular and cellular biology Molecular genetics Molecular Sequence Data OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE Plants, Medicinal - enzymology Plants, Medicinal - genetics Promoter Regions, Genetic RACINE RAICES reporter gene ROOTS Sequence Homology, Nucleic Acid Solanaceae - enzymology Solanaceae - genetics Solanaceae - ultrastructure TATA Box - genetics TRANSCRIPCION TRANSCRIPTION Transcription, Genetic tropane alkaloid tropinone reductase |
| title | Structures and expression patterns of two tropinone reductase genes from Hyoscyamus niger |
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