Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae
Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were clon...
Gespeichert in:
| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-09, Vol.62 (9), p.1774-1777 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1777 |
|---|---|
| container_issue | 9 |
| container_start_page | 1774 |
| container_title | Bioscience, biotechnology, and biochemistry |
| container_volume | 62 |
| creator | Tobimatsu, T. (Okayama Univ. (Japan)) Azuma, M Hayashi, S Nishimoto, K Toraya, T |
| description | Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned nd sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228 and 174 amino acid residues with predicted molecular weights of 60,379(alpha), 24,401(beta), and 19,489(gamma), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported fro diol dehydratases and glycerol dehydratases, respectively |
| doi_str_mv | 10.1271/bbb.62.1774 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1449429023</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3121825761</sourcerecordid><originalsourceid>FETCH-LOGICAL-c567t-d6e0b6d736ea8d9cf88428fa11f0aa5fea3f64abef4d3238dc5d7682238bd0e13</originalsourceid><addsrcrecordid>eNqFkU2P1DAMQCsEWpaFE2ekSCAu0CFp0jQ9ohXfi-AA58hNnJmu0mQ2aYWGf8C_JtUMIHGAUyz72Y71quohoxvWdOzFMAwb2WxY14lb1TnjoqtlL7rb1TntmayVaNnd6l7O15SWRMvOqrNe0ZYrel79-Bg9msVDIsbHMIbtc5LxZsFgSkwgWGJ2kMDMmMbvMI8xkOjIvEOyxYCZuJgIWAwxH7yJA3iYxlBb3GMo2ZnYMXpicXewCWbIuHZ_8DjkEb0Hsg-4TGUv4P3qjgOf8cHpvai-vn715fJtffXpzbvLl1e1aWU311YiHaTtuERQtjdOKdEoB4w5CtA6BO6kgAGdsLzhyprWdlI1JRwsRcYvqqfHufsUy5151tOYzfqZgHHJuqO0UYzy_4KsaxhXcgUf_wVexyWFcoRmQvSi6WmzUs-OlEkx54RO79M4QTpoRvXqURePWjZ69VjoR6eZyzCh_c2exJX6k1MdsgHvEhRh-c9IKTkVfcHkERtDETXBt5i81TMcfEy_evg_9zuIGrapcO8_s77vKWVUtvwnE8jDeA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449429023</pqid></control><display><type>article</type><title>Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae</title><source>J-STAGE Free</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Open Access Titles of Japan</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Tobimatsu, T. (Okayama Univ. (Japan)) ; Azuma, M ; Hayashi, S ; Nishimoto, K ; Toraya, T</creator><creatorcontrib>Tobimatsu, T. (Okayama Univ. (Japan)) ; Azuma, M ; Hayashi, S ; Nishimoto, K ; Toraya, T</creatorcontrib><description>Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned nd sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228 and 174 amino acid residues with predicted molecular weights of 60,379(alpha), 24,401(beta), and 19,489(gamma), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported fro diol dehydratases and glycerol dehydratases, respectively</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.62.1774</identifier><identifier>PMID: 9805380</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>adenosylcobalamin ; Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Bacteriology ; Biological and medical sciences ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning, Molecular ; Cobamides - metabolism ; diol dehydratase ; Enterobacteriaceae ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; glycerol dehydratase ; KLEBSIELLA PNEUMONIAE ; Klebsiella pneumoniae - enzymology ; Klebsiella pneumoniae - genetics ; LIASAS ; LYASE ; LYASES ; Metabolism. Enzymes ; Microbiology ; MOLECULAR CLONING ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Propanediol Dehydratase - genetics ; SECUENCIA NUCLEOTIDICA ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEOTIDIQUE</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998-09, Vol.62 (9), p.1774-1777</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c567t-d6e0b6d736ea8d9cf88428fa11f0aa5fea3f64abef4d3238dc5d7682238bd0e13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1663049$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9805380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tobimatsu, T. (Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Azuma, M</creatorcontrib><creatorcontrib>Hayashi, S</creatorcontrib><creatorcontrib>Nishimoto, K</creatorcontrib><creatorcontrib>Toraya, T</creatorcontrib><title>Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned nd sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228 and 174 amino acid residues with predicted molecular weights of 60,379(alpha), 24,401(beta), and 19,489(gamma), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported fro diol dehydratases and glycerol dehydratases, respectively</description><subject>adenosylcobalamin</subject><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning, Molecular</subject><subject>Cobamides - metabolism</subject><subject>diol dehydratase</subject><subject>Enterobacteriaceae</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>glycerol dehydratase</subject><subject>KLEBSIELLA PNEUMONIAE</subject><subject>Klebsiella pneumoniae - enzymology</subject><subject>Klebsiella pneumoniae - genetics</subject><subject>LIASAS</subject><subject>LYASE</subject><subject>LYASES</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>MOLECULAR CLONING</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Propanediol Dehydratase - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2P1DAMQCsEWpaFE2ekSCAu0CFp0jQ9ohXfi-AA58hNnJmu0mQ2aYWGf8C_JtUMIHGAUyz72Y71quohoxvWdOzFMAwb2WxY14lb1TnjoqtlL7rb1TntmayVaNnd6l7O15SWRMvOqrNe0ZYrel79-Bg9msVDIsbHMIbtc5LxZsFgSkwgWGJ2kMDMmMbvMI8xkOjIvEOyxYCZuJgIWAwxH7yJA3iYxlBb3GMo2ZnYMXpicXewCWbIuHZ_8DjkEb0Hsg-4TGUv4P3qjgOf8cHpvai-vn715fJtffXpzbvLl1e1aWU311YiHaTtuERQtjdOKdEoB4w5CtA6BO6kgAGdsLzhyprWdlI1JRwsRcYvqqfHufsUy5151tOYzfqZgHHJuqO0UYzy_4KsaxhXcgUf_wVexyWFcoRmQvSi6WmzUs-OlEkx54RO79M4QTpoRvXqURePWjZ69VjoR6eZyzCh_c2exJX6k1MdsgHvEhRh-c9IKTkVfcHkERtDETXBt5i81TMcfEy_evg_9zuIGrapcO8_s77vKWVUtvwnE8jDeA</recordid><startdate>19980901</startdate><enddate>19980901</enddate><creator>Tobimatsu, T. (Okayama Univ. (Japan))</creator><creator>Azuma, M</creator><creator>Hayashi, S</creator><creator>Nishimoto, K</creator><creator>Toraya, T</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19980901</creationdate><title>Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae</title><author>Tobimatsu, T. (Okayama Univ. (Japan)) ; Azuma, M ; Hayashi, S ; Nishimoto, K ; Toraya, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c567t-d6e0b6d736ea8d9cf88428fa11f0aa5fea3f64abef4d3238dc5d7682238bd0e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>adenosylcobalamin</topic><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning, Molecular</topic><topic>Cobamides - metabolism</topic><topic>diol dehydratase</topic><topic>Enterobacteriaceae</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>glycerol dehydratase</topic><topic>KLEBSIELLA PNEUMONIAE</topic><topic>Klebsiella pneumoniae - enzymology</topic><topic>Klebsiella pneumoniae - genetics</topic><topic>LIASAS</topic><topic>LYASE</topic><topic>LYASES</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>MOLECULAR CLONING</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Propanediol Dehydratase - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tobimatsu, T. (Okayama Univ. (Japan))</creatorcontrib><creatorcontrib>Azuma, M</creatorcontrib><creatorcontrib>Hayashi, S</creatorcontrib><creatorcontrib>Nishimoto, K</creatorcontrib><creatorcontrib>Toraya, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tobimatsu, T. (Okayama Univ. (Japan))</au><au>Azuma, M</au><au>Hayashi, S</au><au>Nishimoto, K</au><au>Toraya, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1998-09-01</date><risdate>1998</risdate><volume>62</volume><issue>9</issue><spage>1774</spage><epage>1777</epage><pages>1774-1777</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned nd sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228 and 174 amino acid residues with predicted molecular weights of 60,379(alpha), 24,401(beta), and 19,489(gamma), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported fro diol dehydratases and glycerol dehydratases, respectively</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>9805380</pmid><doi>10.1271/bbb.62.1774</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 1998-09, Vol.62 (9), p.1774-1777 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_journals_1449429023 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Open Access Titles of Japan; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | adenosylcobalamin Amino Acid Sequence Analytical, structural and metabolic biochemistry Bacteriology Biological and medical sciences CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular Cobamides - metabolism diol dehydratase Enterobacteriaceae Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Genes, Bacterial glycerol dehydratase KLEBSIELLA PNEUMONIAE Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics LIASAS LYASE LYASES Metabolism. Enzymes Microbiology MOLECULAR CLONING Molecular Sequence Data NUCLEOTIDE SEQUENCE Propanediol Dehydratase - genetics SECUENCIA NUCLEOTIDICA Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE |
| title | Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T18%3A01%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20cloning,%20sequencing%20and%20characterization%20of%20the%20genes%20for%20adenosylcobalamin-dependent%20diol%20dehydratase%20of%20Klebsiella%20pneumoniae&rft.jtitle=Bioscience,%20biotechnology,%20and%20biochemistry&rft.au=Tobimatsu,%20T.%20(Okayama%20Univ.%20(Japan))&rft.date=1998-09-01&rft.volume=62&rft.issue=9&rft.spage=1774&rft.epage=1777&rft.pages=1774-1777&rft.issn=0916-8451&rft.eissn=1347-6947&rft_id=info:doi/10.1271/bbb.62.1774&rft_dat=%3Cproquest_cross%3E3121825761%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449429023&rft_id=info:pmid/9805380&rfr_iscdi=true |