Glutathione transport systems of the budding yeast Saccharomyces cerevisiae
The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport Systems. While one with high affinity (GSH-P1; KT=0.045mM) was regulated, the other with low affinity (GSH-P2; KT2 mM) was not. GSH-P1 was highly specific to ghtathione, and its activit...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-10, Vol.62 (10), p.1858-1864 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Miyake, T. (Okayama-ken. Government Office (Japan)) Hazu, T Yoshida, S Kanayama, M Tomochika, K Shinoda, S Ono, B |
| description | The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport Systems. While one with high affinity (GSH-P1; KT=0.045mM) was regulated, the other with low affinity (GSH-P2; KT2 mM) was not. GSH-P1 was highly specific to ghtathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, p- isolates had only about one half of the glutathione transport activity of the original (p+) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, respectively |
| doi_str_mv | 10.1271/bbb.62.1858 |
| format | Article |
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(Okayama-ken. Government Office (Japan)) ; Hazu, T ; Yoshida, S ; Kanayama, M ; Tomochika, K ; Shinoda, S ; Ono, B</creator><creatorcontrib>Miyake, T. (Okayama-ken. Government Office (Japan)) ; Hazu, T ; Yoshida, S ; Kanayama, M ; Tomochika, K ; Shinoda, S ; Ono, B</creatorcontrib><description>The budding yeast Saccharomyces cerevisiae was shown to have two kinetically distinguishable glutathione transport Systems. While one with high affinity (GSH-P1; KT=0.045mM) was regulated, the other with low affinity (GSH-P2; KT2 mM) was not. GSH-P1 was highly specific to ghtathione, and its activity was quickly lost by suspending the cells in buffer solutions. This activity loss was not observed if glucose-containing buffer was used. In addition, p- isolates had only about one half of the glutathione transport activity of the original (p+) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. 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Molecular biology ; inhibition ; kinetic analysis ; Kinetics ; LEVADURA ; LEVURE ; Microbiology ; Mycology ; NUTRIENT TRANSPORT ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - metabolism ; transport ; TRANSPORT DES SUBSTANCES NUTRITIVES ; TRANSPORTE DE NUTRIENTES ; YEASTS</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1998-10, Vol.62 (10), p.1858-1864</ispartof><rights>1998 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 1998</rights><rights>1999 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c441t-ead0437e3a4e6bc3cb6b4a37e45327c61ea1f902fffc3c232e4648cd922ada473</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1697979$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9836420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miyake, T. 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In addition, p- isolates had only about one half of the glutathione transport activity of the original (p+) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, respectively</description><subject>Adenosine Triphosphate - metabolism</subject><subject>ATP</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active - drug effects</subject><subject>Culture Media</subject><subject>Cysteine - metabolism</subject><subject>Cysteine - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLUTATHION</subject><subject>GLUTATHIONE</subject><subject>Glutathione - metabolism</subject><subject>GLUTATION</subject><subject>Growth, nutrition, metabolism, transports, enzymes. 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This activity loss was not observed if glucose-containing buffer was used. In addition, p- isolates had only about one half of the glutathione transport activity of the original (p+) strain. Therefore, it is concluded that GSH-P1 is an ATP-driven transport system. Strong and moderate inhibition of GSH-P1 by protonophores and ionophores, respectively, are attributed to competition for ATP between GSH-P1 and proton- and cation-pumps, respectively</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>9836420</pmid><doi>10.1271/bbb.62.1858</doi><tpages>7</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1998-10, Vol.62 (10), p.1858-1864 |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Adenosine Triphosphate - metabolism ATP Biological and medical sciences Biological Transport, Active - drug effects Culture Media Cysteine - metabolism Cysteine - pharmacology Fundamental and applied biological sciences. Psychology GLUTATHION GLUTATHIONE Glutathione - metabolism GLUTATION Growth, nutrition, metabolism, transports, enzymes. Molecular biology inhibition kinetic analysis Kinetics LEVADURA LEVURE Microbiology Mycology NUTRIENT TRANSPORT SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - metabolism transport TRANSPORT DES SUBSTANCES NUTRITIVES TRANSPORTE DE NUTRIENTES YEASTS |
| title | Glutathione transport systems of the budding yeast Saccharomyces cerevisiae |
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