Production of Recombinant Der fI with the Native IgE-Binding Activity Using a Baculovirus Expression System

Der fI is a cysteine protease contained in feces of mites and is one of major mite allergens. Recombinant Der fI (reDer fI) that is produced using a baculovirus expression system contains pro-sequences of different lengths. Most of these can be removed by acid treatment. However, IgE-binding activit...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1997, Vol.61 (10), p.1668-1673
Hauptverfasser:	Shoti, Hiroshi, Shibuya, Ichiro, Hirai, Mitsuo, Horiuchi, Hiroyuki, Takagi, Masamichi
Format:	Artikel
Sprache:	eng
Schlagworte:	allergen Animals Antigens - chemistry Antigens - genetics Antigens, Dermatophagoides Baculoviridae baculovirus expression system Binding, Competitive Biological and medical sciences Biotechnology Blotting, Western Culture Media Cysteine Endopeptidases - chemistry Cysteine Endopeptidases - genetics DNA, Complementary - genetics Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Glycoproteins - biosynthesis Glycoproteins - chemistry Glycoproteins - genetics Glycoproteins - isolation & purification Immunoglobulin E - metabolism lectin Lectins - chemistry Lectins - metabolism lysylendopeptidase Methods. Procedures. Technologies Mites Protein engineering Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Recombinant Proteins - genetics Serine Endopeptidases - chemistry
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