Alteration in the Phosphatidylcholine Biosynthesis of Rat Liver Microsomes Caused by Vitamin B6 Deficiency
Rats fed with a vitamin B 6 -deficient 70% casein diet for 5 weeks were found to have decreased considerably in the content of phosphatidylcholine (PC) in liver microsomes, presumably because of the depressed PC biosynthesis from choline or phosphatidylethanolamine (PE). The activities of choline ph...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (2), p.163-167 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | She, Qing-Bai Hayakawa, Takashi Tsuge, Haruhito |
| description | Rats fed with a vitamin B
6
-deficient 70% casein diet for 5 weeks were found to have decreased considerably in the content of phosphatidylcholine (PC) in liver microsomes, presumably because of the depressed PC biosynthesis from choline or phosphatidylethanolamine (PE). The activities of choline phosphokinase and choline phosphotransferase in liver decreased, apparently, as compared with the pair-fed control or control rats. The hepatic level of the PE methyltransferase co-substrate, S-adenosylmethionine (SAM), decreased about 1/3, but the level of the inhibitory metabolite, S-adenosylhomocysteine (SAH), was elevated due to the marked reduction in the activities of cystathionine β-synthase and γ-cystathionase. The resultant molar ratio of SAM/SAH decreased drastically such that the methylation of PE to PC was decreased in vivo, as confirmed by lowering the activity of PE methyltransferase in vitro in response to a decreased molar ratio of SAM/SAH. A similar effect on the PE methylation was also observed in the pair-fed control rats, but the PC biosynthesis from choline clearly compensated for the drop of PC biosynthesis from PE. Results of this study demonstrate that vitamin B
6
deficiency modified methionine metabolism and decreased choline utilization, and thus indirectly affected the biosynthesis of PC in liver microsomes. |
| doi_str_mv | 10.1271/bbb.59.163 |
| format | Article |
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6
-deficient 70% casein diet for 5 weeks were found to have decreased considerably in the content of phosphatidylcholine (PC) in liver microsomes, presumably because of the depressed PC biosynthesis from choline or phosphatidylethanolamine (PE). The activities of choline phosphokinase and choline phosphotransferase in liver decreased, apparently, as compared with the pair-fed control or control rats. The hepatic level of the PE methyltransferase co-substrate, S-adenosylmethionine (SAM), decreased about 1/3, but the level of the inhibitory metabolite, S-adenosylhomocysteine (SAH), was elevated due to the marked reduction in the activities of cystathionine β-synthase and γ-cystathionase. The resultant molar ratio of SAM/SAH decreased drastically such that the methylation of PE to PC was decreased in vivo, as confirmed by lowering the activity of PE methyltransferase in vitro in response to a decreased molar ratio of SAM/SAH. A similar effect on the PE methylation was also observed in the pair-fed control rats, but the PC biosynthesis from choline clearly compensated for the drop of PC biosynthesis from PE. Results of this study demonstrate that vitamin B
6
deficiency modified methionine metabolism and decreased choline utilization, and thus indirectly affected the biosynthesis of PC in liver microsomes.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.59.163</identifier><identifier>PMID: 7766014</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>Animals ; Biological and medical sciences ; Biotechnology ; Caseins - administration & dosage ; Choline - metabolism ; Choline Kinase - metabolism ; Cystathionine beta-Synthase - metabolism ; Cystathionine gamma-Lyase - metabolism ; Diacylglycerol Cholinephosphotransferase - metabolism ; Liver - drug effects ; Liver - enzymology ; Male ; Medical sciences ; Metabolic diseases ; Methylation ; Methyltransferases - metabolism ; Microsomes, Liver - enzymology ; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) ; Phosphatidylcholines - biosynthesis ; Phosphatidylethanolamine N-Methyltransferase ; Phosphatidylethanolamines - metabolism ; Rats ; Rats, Wistar ; S-Adenosylhomocysteine - metabolism ; S-Adenosylmethionine - metabolism ; Vitamin B 6 Deficiency - enzymology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1995, Vol.59 (2), p.163-167</ispartof><rights>Copyright 1995 Taylor and Francis Group LLC 1995</rights><rights>1995 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009,27902,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3481583$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7766014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>She, Qing-Bai</creatorcontrib><creatorcontrib>Hayakawa, Takashi</creatorcontrib><creatorcontrib>Tsuge, Haruhito</creatorcontrib><title>Alteration in the Phosphatidylcholine Biosynthesis of Rat Liver Microsomes Caused by Vitamin B6 Deficiency</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Rats fed with a vitamin B
6
-deficient 70% casein diet for 5 weeks were found to have decreased considerably in the content of phosphatidylcholine (PC) in liver microsomes, presumably because of the depressed PC biosynthesis from choline or phosphatidylethanolamine (PE). The activities of choline phosphokinase and choline phosphotransferase in liver decreased, apparently, as compared with the pair-fed control or control rats. The hepatic level of the PE methyltransferase co-substrate, S-adenosylmethionine (SAM), decreased about 1/3, but the level of the inhibitory metabolite, S-adenosylhomocysteine (SAH), was elevated due to the marked reduction in the activities of cystathionine β-synthase and γ-cystathionase. The resultant molar ratio of SAM/SAH decreased drastically such that the methylation of PE to PC was decreased in vivo, as confirmed by lowering the activity of PE methyltransferase in vitro in response to a decreased molar ratio of SAM/SAH. A similar effect on the PE methylation was also observed in the pair-fed control rats, but the PC biosynthesis from choline clearly compensated for the drop of PC biosynthesis from PE. Results of this study demonstrate that vitamin B
6
deficiency modified methionine metabolism and decreased choline utilization, and thus indirectly affected the biosynthesis of PC in liver microsomes.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Caseins - administration & dosage</subject><subject>Choline - metabolism</subject><subject>Choline Kinase - metabolism</subject><subject>Cystathionine beta-Synthase - metabolism</subject><subject>Cystathionine gamma-Lyase - metabolism</subject><subject>Diacylglycerol Cholinephosphotransferase - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Methylation</subject><subject>Methyltransferases - metabolism</subject><subject>Microsomes, Liver - enzymology</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>Phosphatidylcholines - biosynthesis</subject><subject>Phosphatidylethanolamine N-Methyltransferase</subject><subject>Phosphatidylethanolamines - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>S-Adenosylhomocysteine - metabolism</subject><subject>S-Adenosylmethionine - metabolism</subject><subject>Vitamin B 6 Deficiency - enzymology</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2LFDEQxYMo67h68S4EFG89m3TSSee4O34tjCiiXkOSrjAZ0p0x6V7p_97Ijh72VPDerx5FPYReUrKlraRX1tptp7ZUsEdoQxmXjVBcPkYboqhoet7Rp-hZKUdCqtDRC3QhpRCE8g06XscZsplDmnCY8HwA_PWQyulQpWGN7pBimADfhFTWqbolFJw8_mZmvA93kPHn4HIqaYSCd2YpMGC74p9hNmONuxH4HfjgAkxufY6eeBMLvDjPS_Tjw_vvu0_N_svH2931vgmslXPjlQROJbQWnO1bY5kXngjhnOuUNN6K3lHpvOJWAVjbMmcG21Ev5OCAE3aJ3t7nnnL6tUCZ9RiKgxjNBGkpWsqW0L6XFXz9ADymJU_1Nk05V5xI1olKvTpTix1h0KccRpNXfX5h9d-cfVOciT6byYXyH2O8p13PKtbdY2HyKY_md8px0LNZY8r_dhgl-m-huhaqO6VroewP6huS1g</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>She, Qing-Bai</creator><creator>Hayakawa, Takashi</creator><creator>Tsuge, Haruhito</creator><general>Taylor & Francis</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1995</creationdate><title>Alteration in the Phosphatidylcholine Biosynthesis of Rat Liver Microsomes Caused by Vitamin B6 Deficiency</title><author>She, Qing-Bai ; Hayakawa, Takashi ; Tsuge, Haruhito</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i327t-f97e417e2becb82ab3f6f066ccc597afb68c17cf94b9eebb23cadb51f67dce403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Caseins - administration & dosage</topic><topic>Choline - metabolism</topic><topic>Choline Kinase - metabolism</topic><topic>Cystathionine beta-Synthase - metabolism</topic><topic>Cystathionine gamma-Lyase - metabolism</topic><topic>Diacylglycerol Cholinephosphotransferase - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Methylation</topic><topic>Methyltransferases - metabolism</topic><topic>Microsomes, Liver - enzymology</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>Phosphatidylcholines - biosynthesis</topic><topic>Phosphatidylethanolamine N-Methyltransferase</topic><topic>Phosphatidylethanolamines - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>S-Adenosylhomocysteine - metabolism</topic><topic>S-Adenosylmethionine - metabolism</topic><topic>Vitamin B 6 Deficiency - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>She, Qing-Bai</creatorcontrib><creatorcontrib>Hayakawa, Takashi</creatorcontrib><creatorcontrib>Tsuge, Haruhito</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>She, Qing-Bai</au><au>Hayakawa, Takashi</au><au>Tsuge, Haruhito</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alteration in the Phosphatidylcholine Biosynthesis of Rat Liver Microsomes Caused by Vitamin B6 Deficiency</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>1995</date><risdate>1995</risdate><volume>59</volume><issue>2</issue><spage>163</spage><epage>167</epage><pages>163-167</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Rats fed with a vitamin B
6
-deficient 70% casein diet for 5 weeks were found to have decreased considerably in the content of phosphatidylcholine (PC) in liver microsomes, presumably because of the depressed PC biosynthesis from choline or phosphatidylethanolamine (PE). The activities of choline phosphokinase and choline phosphotransferase in liver decreased, apparently, as compared with the pair-fed control or control rats. The hepatic level of the PE methyltransferase co-substrate, S-adenosylmethionine (SAM), decreased about 1/3, but the level of the inhibitory metabolite, S-adenosylhomocysteine (SAH), was elevated due to the marked reduction in the activities of cystathionine β-synthase and γ-cystathionase. The resultant molar ratio of SAM/SAH decreased drastically such that the methylation of PE to PC was decreased in vivo, as confirmed by lowering the activity of PE methyltransferase in vitro in response to a decreased molar ratio of SAM/SAH. A similar effect on the PE methylation was also observed in the pair-fed control rats, but the PC biosynthesis from choline clearly compensated for the drop of PC biosynthesis from PE. Results of this study demonstrate that vitamin B
6
deficiency modified methionine metabolism and decreased choline utilization, and thus indirectly affected the biosynthesis of PC in liver microsomes.</abstract><cop>Tokyo</cop><pub>Taylor & Francis</pub><pmid>7766014</pmid><doi>10.1271/bbb.59.163</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1995, Vol.59 (2), p.163-167 |
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| source | Freely Accessible Japanese Titles - check A-Z of ejournals; J-STAGE Free; MEDLINE; Full-Text Journals in Chemistry (Open access); EZB Electronic Journals Library |
| subjects | Animals Biological and medical sciences Biotechnology Caseins - administration & dosage Choline - metabolism Choline Kinase - metabolism Cystathionine beta-Synthase - metabolism Cystathionine gamma-Lyase - metabolism Diacylglycerol Cholinephosphotransferase - metabolism Liver - drug effects Liver - enzymology Male Medical sciences Metabolic diseases Methylation Methyltransferases - metabolism Microsomes, Liver - enzymology Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) Phosphatidylcholines - biosynthesis Phosphatidylethanolamine N-Methyltransferase Phosphatidylethanolamines - metabolism Rats Rats, Wistar S-Adenosylhomocysteine - metabolism S-Adenosylmethionine - metabolism Vitamin B 6 Deficiency - enzymology |
| title | Alteration in the Phosphatidylcholine Biosynthesis of Rat Liver Microsomes Caused by Vitamin B6 Deficiency |
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