Purification and characterization of copper-metallothioneins from Aspergillus niger
Copper metallothioneins were extracted from Aspergillus niger after an induction period of five hours in a copper-containing suspension medium. The crude extract (FI) was obtained by cell homogenization and the partially purified extract (FII) was obtained by heat treatment at 60°C for 10 min. The p...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1993, Vol.57 (9), p.1420-1423 |
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| container_issue | 9 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Kermasha, S. (McGill Univ., Quebec (Canada)) Pellerin, F Rovel, B Goetghebeur, M Metche, M |
| description | Copper metallothioneins were extracted from Aspergillus niger after an induction period of five hours in a copper-containing suspension medium. The crude extract (FI) was obtained by cell homogenization and the partially purified extract (FII) was obtained by heat treatment at 60°C for 10 min. The partially purified extract (FII) was further purified by successive chromatographies on size-exclusion (Biogel P-30) and ion-exchange (TSK-HW 65-DEAE) chromatography columns. All protein fractions were analyzed for their protein and induced copper contents. The Cu/protein ratio increased steadily throughout the purification process; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6. |
| doi_str_mv | 10.1271/bbb.57.1420 |
| format | Article |
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(McGill Univ., Quebec (Canada)) ; Pellerin, F ; Rovel, B ; Goetghebeur, M ; Metche, M</creator><creatorcontrib>Kermasha, S. (McGill Univ., Quebec (Canada)) ; Pellerin, F ; Rovel, B ; Goetghebeur, M ; Metche, M</creatorcontrib><description>Copper metallothioneins were extracted from Aspergillus niger after an induction period of five hours in a copper-containing suspension medium. The crude extract (FI) was obtained by cell homogenization and the partially purified extract (FII) was obtained by heat treatment at 60°C for 10 min. The partially purified extract (FII) was further purified by successive chromatographies on size-exclusion (Biogel P-30) and ion-exchange (TSK-HW 65-DEAE) chromatography columns. All protein fractions were analyzed for their protein and induced copper contents. The Cu/protein ratio increased steadily throughout the purification process; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.57.1420</identifier><language>eng</language><publisher>Tokyo: Taylor & Francis</publisher><subject>ALMACENAMIENTO ; ASPERGILLUS NIGER ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CHEMICOPHYSICAL PROPERTIES ; COBRE ; CONTENIDO MINERAL ; COPPER ; CUIVRE ; DESINTOXICACION ; DETOXIFICATION ; Fundamental and applied biological sciences. Psychology ; METALLOPROTEINE ; METALLOPROTEINS ; METALPROTEINAS ; MINERAL CONTENT ; Mission oriented research ; Physiology and metabolism ; PROPIEDADES FISICO-QUIMICAS ; PROPRIETE PHYSICOCHIMIQUE ; PURIFICACION ; PURIFICATION ; STOCKAGE ; STORAGE ; TENEUR EN ELEMENTS MINERAUX</subject><ispartof>Bioscience, biotechnology, and biochemistry, 1993, Vol.57 (9), p.1420-1423</ispartof><rights>Copyright 1993 Taylor and Francis Group LLC 1993</rights><rights>1994 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-591a4429e5fc6f7a7a51f868984d84d508e6141bf361cd290c71f9777eeb357a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4095356$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kermasha, S. 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The Cu/protein ratio increased steadily throughout the purification process; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6.</description><subject>ALMACENAMIENTO</subject><subject>ASPERGILLUS NIGER</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CHEMICOPHYSICAL PROPERTIES</subject><subject>COBRE</subject><subject>CONTENIDO MINERAL</subject><subject>COPPER</subject><subject>CUIVRE</subject><subject>DESINTOXICACION</subject><subject>DETOXIFICATION</subject><subject>Fundamental and applied biological sciences. 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The partially purified extract (FII) was further purified by successive chromatographies on size-exclusion (Biogel P-30) and ion-exchange (TSK-HW 65-DEAE) chromatography columns. All protein fractions were analyzed for their protein and induced copper contents. The Cu/protein ratio increased steadily throughout the purification process; the most purified fraction (FIV) showed a 24-fold increase, with a recovery of 13%. The molecular weight of the Cu-metallothionein fraction FIV was 11,000 and the ratio of moles of copper per mole of protein was 6.6.</abstract><cop>Tokyo</cop><pub>Taylor & Francis</pub><doi>10.1271/bbb.57.1420</doi><tpages>4</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 1993, Vol.57 (9), p.1420-1423 |
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| source | J-STAGE Free; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | ALMACENAMIENTO ASPERGILLUS NIGER Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CHEMICOPHYSICAL PROPERTIES COBRE CONTENIDO MINERAL COPPER CUIVRE DESINTOXICACION DETOXIFICATION Fundamental and applied biological sciences. Psychology METALLOPROTEINE METALLOPROTEINS METALPROTEINAS MINERAL CONTENT Mission oriented research Physiology and metabolism PROPIEDADES FISICO-QUIMICAS PROPRIETE PHYSICOCHIMIQUE PURIFICACION PURIFICATION STOCKAGE STORAGE TENEUR EN ELEMENTS MINERAUX |
| title | Purification and characterization of copper-metallothioneins from Aspergillus niger |
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