Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide
This study was designed to examine the in vitro effects of adenosine (Ado) on hydrogen peroxide-induced endothelial dysfunction in rats. Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protecti...
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| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2007, Vol.30(7), pp.1206-1211 |
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| creator | Lu, Jun Zhu, Shu-Ming Zang, Wei-Jin Xu, Xiao-Li Luo, Hong-Li Yu, Xiao-Jiang Wang, Sheng-Peng Kong, Shan-Shan Wu, Jie Horie, Minoru Sun, Lei |
| description | This study was designed to examine the in vitro effects of adenosine (Ado) on hydrogen peroxide-induced endothelial dysfunction in rats. Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protective effects of adenosine were tested by exposing isolated mesenteric arteries to adenosine (3×10−7 mol/l, 10−6 mol/l, 3×10−6 mol/l)+hydrogen peroxide (0.5 mM) for 12 h. This exposure to hydrogen peroxide induced a significant concentration-dependent inhibition of endothelium-dependent relaxation (EDR). Coculture of segments of mesenteric artery with adenosine (3×10−7, 10−6, and 3×10−6 mol/l) attenuated the hydrogen peroxide-induced impairment of vasorelaxation. This impairment was accompanied by a reduction in nitrite/nitrate, nitric oxide (NO) synthase (NOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and an increasing in malondislehyde (MDA) and lactate dehydrogenase (LDH) activities in the aorta. These results indicate that adenosine can be used to attenuate hydrogen peroxide-induced endothelial dysfunction, an effect that may be related to antioxidation, thus enhancing NO production by preventing the decrease in NOS. |
| doi_str_mv | 10.1248/bpb.30.1206 |
| format | Article |
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Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protective effects of adenosine were tested by exposing isolated mesenteric arteries to adenosine (3×10−7 mol/l, 10−6 mol/l, 3×10−6 mol/l)+hydrogen peroxide (0.5 mM) for 12 h. This exposure to hydrogen peroxide induced a significant concentration-dependent inhibition of endothelium-dependent relaxation (EDR). Coculture of segments of mesenteric artery with adenosine (3×10−7, 10−6, and 3×10−6 mol/l) attenuated the hydrogen peroxide-induced impairment of vasorelaxation. This impairment was accompanied by a reduction in nitrite/nitrate, nitric oxide (NO) synthase (NOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and an increasing in malondislehyde (MDA) and lactate dehydrogenase (LDH) activities in the aorta. These results indicate that adenosine can be used to attenuate hydrogen peroxide-induced endothelial dysfunction, an effect that may be related to antioxidation, thus enhancing NO production by preventing the decrease in NOS.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.30.1206</identifier><identifier>PMID: 17603154</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>adenosine ; Adenosine - pharmacology ; Animals ; Aorta, Thoracic - drug effects ; Aorta, Thoracic - metabolism ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - physiology ; endothelium-dependent relaxation (EDR) ; Female ; hydrogen peroxide ; Hydrogen Peroxide - toxicity ; Lipid Peroxidation - drug effects ; Male ; Mesenteric Arteries - drug effects ; Mesenteric Arteries - physiology ; nitric oxide (NO) ; Nitric Oxide - biosynthesis ; nitric oxide synthase (NOS) ; Nitric Oxide Synthase - analysis ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Biological and Pharmaceutical Bulletin, 2007, Vol.30(7), pp.1206-1211</ispartof><rights>2007 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c688t-6a916042ace386f42b0197ad0c509ec6e0a955d8f68495e2b773e29904450e293</citedby><cites>FETCH-LOGICAL-c688t-6a916042ace386f42b0197ad0c509ec6e0a955d8f68495e2b773e29904450e293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1876,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17603154$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Jun</creatorcontrib><creatorcontrib>Zhu, Shu-Ming</creatorcontrib><creatorcontrib>Zang, Wei-Jin</creatorcontrib><creatorcontrib>Xu, Xiao-Li</creatorcontrib><creatorcontrib>Luo, Hong-Li</creatorcontrib><creatorcontrib>Yu, Xiao-Jiang</creatorcontrib><creatorcontrib>Wang, Sheng-Peng</creatorcontrib><creatorcontrib>Kong, Shan-Shan</creatorcontrib><creatorcontrib>Wu, Jie</creatorcontrib><creatorcontrib>Horie, Minoru</creatorcontrib><creatorcontrib>Sun, Lei</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>cDepartment of Cardiovascular and Respiratory Medicine</creatorcontrib><creatorcontrib>aDepartment of Pharmacology</creatorcontrib><creatorcontrib>bKey Laboratory of Environment and Genes Related to Diseases of Ministry of Education</creatorcontrib><creatorcontrib>Xi'an Jiaotong University</creatorcontrib><creatorcontrib>Shiga University of Medical Science</creatorcontrib><title>Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>This study was designed to examine the in vitro effects of adenosine (Ado) on hydrogen peroxide-induced endothelial dysfunction in rats. Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protective effects of adenosine were tested by exposing isolated mesenteric arteries to adenosine (3×10−7 mol/l, 10−6 mol/l, 3×10−6 mol/l)+hydrogen peroxide (0.5 mM) for 12 h. This exposure to hydrogen peroxide induced a significant concentration-dependent inhibition of endothelium-dependent relaxation (EDR). Coculture of segments of mesenteric artery with adenosine (3×10−7, 10−6, and 3×10−6 mol/l) attenuated the hydrogen peroxide-induced impairment of vasorelaxation. This impairment was accompanied by a reduction in nitrite/nitrate, nitric oxide (NO) synthase (NOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and an increasing in malondislehyde (MDA) and lactate dehydrogenase (LDH) activities in the aorta. These results indicate that adenosine can be used to attenuate hydrogen peroxide-induced endothelial dysfunction, an effect that may be related to antioxidation, thus enhancing NO production by preventing the decrease in NOS.</description><subject>adenosine</subject><subject>Adenosine - pharmacology</subject><subject>Animals</subject><subject>Aorta, Thoracic - drug effects</subject><subject>Aorta, Thoracic - metabolism</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - physiology</subject><subject>endothelium-dependent relaxation (EDR)</subject><subject>Female</subject><subject>hydrogen peroxide</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Male</subject><subject>Mesenteric Arteries - drug effects</subject><subject>Mesenteric Arteries - physiology</subject><subject>nitric oxide (NO)</subject><subject>Nitric Oxide - biosynthesis</subject><subject>nitric oxide synthase (NOS)</subject><subject>Nitric Oxide Synthase - analysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUcFu1DAQtRCILoUTd2SJI0oZx45j31iVllYqokJwthxn0maVtRfbqdi_x9ts6WXmyfP83ugNIe8ZnLFaqM_drjvjBwzyBVkxLtqqqVnzkqxAM1VJ1qgT8ialDQC0UPPX5IS1EjhrxIpsbmPI6PL4gPQ7unvrx7SlYaDrHn1Io0eaA833SH_aTNcxYxztRC98H8rjdMBf92mYfZEInl77fnbY025Pr_Z9DHfo6S3G8Hfs8S15Ndgp4btjPyW_Ly9-nV9VNz--XZ-vbyonlcqVtJpJELV1yJUcRN0B063twTWg0UkEq5umV4NUQjdYd23LsdYahGigAH5KPi66uxj-zJiy2YQ5-mJpmBCal0BaKKxPC8vFkFLEweziuLVxbxiYQ66m5Gr4AYMs7A9HzbnbYv_MPQZZCJcLoUxHZ6fgp5Lds7NLbTeGKZi6HMEA8KWJR_lSGJNa1eqw_ZdFaJOyvcP_Tjbm0U34tFW7lMffT6NyvWjQ83-dqZ_e</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Lu, Jun</creator><creator>Zhu, Shu-Ming</creator><creator>Zang, Wei-Jin</creator><creator>Xu, Xiao-Li</creator><creator>Luo, Hong-Li</creator><creator>Yu, Xiao-Jiang</creator><creator>Wang, Sheng-Peng</creator><creator>Kong, Shan-Shan</creator><creator>Wu, Jie</creator><creator>Horie, Minoru</creator><creator>Sun, Lei</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>20070701</creationdate><title>Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide</title><author>Lu, Jun ; Zhu, Shu-Ming ; Zang, Wei-Jin ; Xu, Xiao-Li ; Luo, Hong-Li ; Yu, Xiao-Jiang ; Wang, Sheng-Peng ; Kong, Shan-Shan ; Wu, Jie ; Horie, Minoru ; Sun, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c688t-6a916042ace386f42b0197ad0c509ec6e0a955d8f68495e2b773e29904450e293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>adenosine</topic><topic>Adenosine - pharmacology</topic><topic>Animals</topic><topic>Aorta, Thoracic - drug effects</topic><topic>Aorta, Thoracic - metabolism</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - physiology</topic><topic>endothelium-dependent relaxation (EDR)</topic><topic>Female</topic><topic>hydrogen peroxide</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Male</topic><topic>Mesenteric Arteries - drug effects</topic><topic>Mesenteric Arteries - physiology</topic><topic>nitric oxide (NO)</topic><topic>Nitric Oxide - biosynthesis</topic><topic>nitric oxide synthase (NOS)</topic><topic>Nitric Oxide Synthase - analysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Jun</creatorcontrib><creatorcontrib>Zhu, Shu-Ming</creatorcontrib><creatorcontrib>Zang, Wei-Jin</creatorcontrib><creatorcontrib>Xu, Xiao-Li</creatorcontrib><creatorcontrib>Luo, Hong-Li</creatorcontrib><creatorcontrib>Yu, Xiao-Jiang</creatorcontrib><creatorcontrib>Wang, Sheng-Peng</creatorcontrib><creatorcontrib>Kong, Shan-Shan</creatorcontrib><creatorcontrib>Wu, Jie</creatorcontrib><creatorcontrib>Horie, Minoru</creatorcontrib><creatorcontrib>Sun, Lei</creatorcontrib><creatorcontrib>School of Medicine</creatorcontrib><creatorcontrib>cDepartment of Cardiovascular and Respiratory Medicine</creatorcontrib><creatorcontrib>aDepartment of Pharmacology</creatorcontrib><creatorcontrib>bKey Laboratory of Environment and Genes Related to Diseases of Ministry of Education</creatorcontrib><creatorcontrib>Xi'an Jiaotong University</creatorcontrib><creatorcontrib>Shiga University of Medical Science</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Jun</au><au>Zhu, Shu-Ming</au><au>Zang, Wei-Jin</au><au>Xu, Xiao-Li</au><au>Luo, Hong-Li</au><au>Yu, Xiao-Jiang</au><au>Wang, Sheng-Peng</au><au>Kong, Shan-Shan</au><au>Wu, Jie</au><au>Horie, Minoru</au><au>Sun, Lei</au><aucorp>School of Medicine</aucorp><aucorp>cDepartment of Cardiovascular and Respiratory Medicine</aucorp><aucorp>aDepartment of Pharmacology</aucorp><aucorp>bKey Laboratory of Environment and Genes Related to Diseases of Ministry of Education</aucorp><aucorp>Xi'an Jiaotong University</aucorp><aucorp>Shiga University of Medical Science</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>30</volume><issue>7</issue><spage>1206</spage><epage>1211</epage><pages>1206-1211</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>This study was designed to examine the in vitro effects of adenosine (Ado) on hydrogen peroxide-induced endothelial dysfunction in rats. Endothelial dysfunction was induced by exposing isolated rat mesenteric arteries to hydrogen peroxide (0.5 mM) for 12 h using an organ culture system. The protective effects of adenosine were tested by exposing isolated mesenteric arteries to adenosine (3×10−7 mol/l, 10−6 mol/l, 3×10−6 mol/l)+hydrogen peroxide (0.5 mM) for 12 h. This exposure to hydrogen peroxide induced a significant concentration-dependent inhibition of endothelium-dependent relaxation (EDR). Coculture of segments of mesenteric artery with adenosine (3×10−7, 10−6, and 3×10−6 mol/l) attenuated the hydrogen peroxide-induced impairment of vasorelaxation. This impairment was accompanied by a reduction in nitrite/nitrate, nitric oxide (NO) synthase (NOS), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and an increasing in malondislehyde (MDA) and lactate dehydrogenase (LDH) activities in the aorta. These results indicate that adenosine can be used to attenuate hydrogen peroxide-induced endothelial dysfunction, an effect that may be related to antioxidation, thus enhancing NO production by preventing the decrease in NOS.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>17603154</pmid><doi>10.1248/bpb.30.1206</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | adenosine Adenosine - pharmacology Animals Aorta, Thoracic - drug effects Aorta, Thoracic - metabolism Endothelium, Vascular - drug effects Endothelium, Vascular - physiology endothelium-dependent relaxation (EDR) Female hydrogen peroxide Hydrogen Peroxide - toxicity Lipid Peroxidation - drug effects Male Mesenteric Arteries - drug effects Mesenteric Arteries - physiology nitric oxide (NO) Nitric Oxide - biosynthesis nitric oxide synthase (NOS) Nitric Oxide Synthase - analysis Rats Rats, Sprague-Dawley |
| title | Protective Mechanism of Adenosine to the Rat Arterial Endothelial Dysfunction Induced by Hydrogen Peroxide |
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