Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane
The permeability of insulin across Xenopus pulmonary membrane and the effects of various absorption enhancers on insulin permeability were examined using an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium sali...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2001, Vol.24(4), pp.385-389 |
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| creator | YAMAMOTO, Akira TANAKA, Hideaki OKUMURA, Shigeki SHINSAKO, Keiko ITO, Mayuko YAMASHITA, Masahiro OKADA, Naoki FUJITA, Takuya MURANISHI, Shozo |
| description | The permeability of insulin across Xenopus pulmonary membrane and the effects of various absorption enhancers on insulin permeability were examined using an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium salicylate (NaSal) and ethylenediaminetetraacetic acid disodium salt (EDTA). The permeability of insulin across Xenopus pulmonary membrane significantly increased in the presence of NaCap and NaGC, while EDTA and NaSal did not enhance the permeability. In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistance (Rm) of Xenopus lung was markedly decreased in the presence of these enhancers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) released from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC improve the pulmonary absorption of insulin, but they are toxic to the pulmonary membrane. These findings suggest that this method is useful for estimating the permeability characteristics of peptides across the pulmonary membrane and for evaluating the effects of various additives on their permeability and their membrane toxicity. |
| doi_str_mv | 10.1248/bpb.24.385 |
| format | Article |
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Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium salicylate (NaSal) and ethylenediaminetetraacetic acid disodium salt (EDTA). The permeability of insulin across Xenopus pulmonary membrane significantly increased in the presence of NaCap and NaGC, while EDTA and NaSal did not enhance the permeability. In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistance (Rm) of Xenopus lung was markedly decreased in the presence of these enhancers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) released from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC improve the pulmonary absorption of insulin, but they are toxic to the pulmonary membrane. These findings suggest that this method is useful for estimating the permeability characteristics of peptides across the pulmonary membrane and for evaluating the effects of various additives on their permeability and their membrane toxicity.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.24.385</identifier><identifier>PMID: 11305600</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Absorption ; absorption enhancer ; Animals ; Biological and medical sciences ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Female ; General pharmacology ; Hormones. Endocrine system ; Hypoglycemic Agents - pharmacokinetics ; In Vitro Techniques ; insulin ; Insulin - pharmacokinetics ; L-Lactate Dehydrogenase - metabolism ; Lung - drug effects ; Lung - enzymology ; Lung - metabolism ; Medical sciences ; Membranes - enzymology ; Membranes - metabolism ; Patch-Clamp Techniques ; peptide delivery ; Permeability ; Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions ; Pharmacology. Drug treatments ; pulmonary absorption ; Xenopus laevis ; Xenopus lung</subject><ispartof>Biological and Pharmaceutical Bulletin, 2001, Vol.24(4), pp.385-389</ispartof><rights>2001 The Pharmaceutical Society of Japan</rights><rights>2001 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c623t-f04158150590c14d1dd98d8c62b5135d6b4327d1a430efc13a5882c011f512423</citedby><cites>FETCH-LOGICAL-c623t-f04158150590c14d1dd98d8c62b5135d6b4327d1a430efc13a5882c011f512423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=967988$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11305600$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAMAMOTO, Akira</creatorcontrib><creatorcontrib>TANAKA, Hideaki</creatorcontrib><creatorcontrib>OKUMURA, Shigeki</creatorcontrib><creatorcontrib>SHINSAKO, Keiko</creatorcontrib><creatorcontrib>ITO, Mayuko</creatorcontrib><creatorcontrib>YAMASHITA, Masahiro</creatorcontrib><creatorcontrib>OKADA, Naoki</creatorcontrib><creatorcontrib>FUJITA, Takuya</creatorcontrib><creatorcontrib>MURANISHI, Shozo</creatorcontrib><title>Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The permeability of insulin across Xenopus pulmonary membrane and the effects of various absorption enhancers on insulin permeability were examined using an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium salicylate (NaSal) and ethylenediaminetetraacetic acid disodium salt (EDTA). The permeability of insulin across Xenopus pulmonary membrane significantly increased in the presence of NaCap and NaGC, while EDTA and NaSal did not enhance the permeability. In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistance (Rm) of Xenopus lung was markedly decreased in the presence of these enhancers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) released from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC improve the pulmonary absorption of insulin, but they are toxic to the pulmonary membrane. These findings suggest that this method is useful for estimating the permeability characteristics of peptides across the pulmonary membrane and for evaluating the effects of various additives on their permeability and their membrane toxicity.</description><subject>Absorption</subject><subject>absorption enhancer</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Hormones. Endocrine system</subject><subject>Hypoglycemic Agents - pharmacokinetics</subject><subject>In Vitro Techniques</subject><subject>insulin</subject><subject>Insulin - pharmacokinetics</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Lung - drug effects</subject><subject>Lung - enzymology</subject><subject>Lung - metabolism</subject><subject>Medical sciences</subject><subject>Membranes - enzymology</subject><subject>Membranes - metabolism</subject><subject>Patch-Clamp Techniques</subject><subject>peptide delivery</subject><subject>Permeability</subject><subject>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</subject><subject>Pharmacology. Drug treatments</subject><subject>pulmonary absorption</subject><subject>Xenopus laevis</subject><subject>Xenopus lung</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkd9u0zAUxi3ExMrghgdAlrhDSufjP6lzhaYpQKWhTYIh7izHcVZXiRNsB6kvxHPi0lK2G1vy9zvn8zkfQm-ALIFyedlMzZLyJZPiGVoA46tCUBDP0YJUIIsShDxHL2PcEkJWhLIX6ByAEVESskC_61-6n3Vyo8djh9c-zr3z-M6GwerG9S7tsPYtrrvOmhT3zFUTxzD9raj9RntjQ373eJ3lJ3XNvhTnbt9dCiO-m_th9DrscD25tLG90z3-uovJDvg-Ov-Af1g_TnN8RH6xQxO0t6_QWaf7aF8f7wt0_7H-dv25uLn9tL6-uilMSVkqOsLztCCIqIgB3kLbVrKVWWwEMNGWDWd01YLmjNjOANNCSmoIQCfyKim7QO8Ofacw_pxtTGo7zsFnSwWc53VWQESm3h8oE8YYg-3UFNyQ_6uAqH0kKkeiKFc5kgy_Pbacm8G2_9FjBo88dTS67_K8xsUTV5WrSspMfThQ25j0gz3JOiRnevvPkR-ObHxSzEYHZT37A4esq-c</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>YAMAMOTO, Akira</creator><creator>TANAKA, Hideaki</creator><creator>OKUMURA, Shigeki</creator><creator>SHINSAKO, Keiko</creator><creator>ITO, Mayuko</creator><creator>YAMASHITA, Masahiro</creator><creator>OKADA, Naoki</creator><creator>FUJITA, Takuya</creator><creator>MURANISHI, Shozo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>2001</creationdate><title>Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane</title><author>YAMAMOTO, Akira ; TANAKA, Hideaki ; OKUMURA, Shigeki ; SHINSAKO, Keiko ; ITO, Mayuko ; YAMASHITA, Masahiro ; OKADA, Naoki ; FUJITA, Takuya ; MURANISHI, Shozo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c623t-f04158150590c14d1dd98d8c62b5135d6b4327d1a430efc13a5882c011f512423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Absorption</topic><topic>absorption enhancer</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Hormones. Endocrine system</topic><topic>Hypoglycemic Agents - pharmacokinetics</topic><topic>In Vitro Techniques</topic><topic>insulin</topic><topic>Insulin - pharmacokinetics</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Lung - drug effects</topic><topic>Lung - enzymology</topic><topic>Lung - metabolism</topic><topic>Medical sciences</topic><topic>Membranes - enzymology</topic><topic>Membranes - metabolism</topic><topic>Patch-Clamp Techniques</topic><topic>peptide delivery</topic><topic>Permeability</topic><topic>Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions</topic><topic>Pharmacology. Drug treatments</topic><topic>pulmonary absorption</topic><topic>Xenopus laevis</topic><topic>Xenopus lung</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAMAMOTO, Akira</creatorcontrib><creatorcontrib>TANAKA, Hideaki</creatorcontrib><creatorcontrib>OKUMURA, Shigeki</creatorcontrib><creatorcontrib>SHINSAKO, Keiko</creatorcontrib><creatorcontrib>ITO, Mayuko</creatorcontrib><creatorcontrib>YAMASHITA, Masahiro</creatorcontrib><creatorcontrib>OKADA, Naoki</creatorcontrib><creatorcontrib>FUJITA, Takuya</creatorcontrib><creatorcontrib>MURANISHI, Shozo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAMAMOTO, Akira</au><au>TANAKA, Hideaki</au><au>OKUMURA, Shigeki</au><au>SHINSAKO, Keiko</au><au>ITO, Mayuko</au><au>YAMASHITA, Masahiro</au><au>OKADA, Naoki</au><au>FUJITA, Takuya</au><au>MURANISHI, Shozo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2001</date><risdate>2001</risdate><volume>24</volume><issue>4</issue><spage>385</spage><epage>389</epage><pages>385-389</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The permeability of insulin across Xenopus pulmonary membrane and the effects of various absorption enhancers on insulin permeability were examined using an in vitro Ussing chamber technique. Absorption enhancers used in this study were sodium caprate (NaCap), sodium glycocholate (NaGC), sodium salicylate (NaSal) and ethylenediaminetetraacetic acid disodium salt (EDTA). The permeability of insulin across Xenopus pulmonary membrane significantly increased in the presence of NaCap and NaGC, while EDTA and NaSal did not enhance the permeability. In addition, the enhancing effect of NaGC increased as the concentrations of these enhancers increased. Transmembrane resistance (Rm) of Xenopus lung was markedly decreased in the presence of these enhancers, and NaCap showed a greater effect on Rm than NaGC. Furthermore, the amount of alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) released from the apical side of the Xenopus pulmonary membrane increased in the presence of these enhancers. These results indicate that NaCap and NaGC improve the pulmonary absorption of insulin, but they are toxic to the pulmonary membrane. These findings suggest that this method is useful for estimating the permeability characteristics of peptides across the pulmonary membrane and for evaluating the effects of various additives on their permeability and their membrane toxicity.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>11305600</pmid><doi>10.1248/bpb.24.385</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry |
| subjects | Absorption absorption enhancer Animals Biological and medical sciences Epithelial Cells - drug effects Epithelial Cells - metabolism Female General pharmacology Hormones. Endocrine system Hypoglycemic Agents - pharmacokinetics In Vitro Techniques insulin Insulin - pharmacokinetics L-Lactate Dehydrogenase - metabolism Lung - drug effects Lung - enzymology Lung - metabolism Medical sciences Membranes - enzymology Membranes - metabolism Patch-Clamp Techniques peptide delivery Permeability Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions Pharmacology. Drug treatments pulmonary absorption Xenopus laevis Xenopus lung |
| title | Evaluation of Insulin Permeability and Effects of Absorption Enhancers on Its Permeability by an in Vitro Pulmonary Epithelial System Using Xenopus Pulmonary Membrane |
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