Hemoglobin Denaturation Caused by Surfactants
The properties of hemoglobin denaturation caused by two anionic surfactants (sodium dodecylsulfate and sodium lauroylmethyltaurate) were examined using spectrophotometry (multi-plate reader), circular dichroism (CD) and high-performance liquid chromatography (HPLC), and their denaturating action on...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1995/04/15, Vol.18(4), pp.540-543 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | HAYASHI, Toshikatsu ITAGAKI, Hiroshi FUKUDA, Toshio TAMURA, Uhei SATO, Yukio SUZUKI, Yasuo |
| description | The properties of hemoglobin denaturation caused by two anionic surfactants (sodium dodecylsulfate and sodium lauroylmethyltaurate) were examined using spectrophotometry (multi-plate reader), circular dichroism (CD) and high-performance liquid chromatography (HPLC), and their denaturating action on hemoglobin and sorption to it were examined by various methods. Correlation and factorial analyses were applied to the experimental data and the following results were obtained : (1) High correlations were found among sorption, denaturation and α-helix content and the random structure of hemoglobin. (2) The α-helix of hemoglobin is randomized as the surfactants denature hemoglobin. (3) Factorial analysis indicates that there are two factors involved in hemoglobin denaturation, one related to the destruction of the α-helix and the other related to a change in the β-structure. These two factors are related to change in the environment around the heme group. (4) Destruction of the α-helix seems to be one of the causes of the eye irritation produced by anionic surfactants. |
| doi_str_mv | 10.1248/bpb.18.540 |
| format | Article |
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Correlation and factorial analyses were applied to the experimental data and the following results were obtained : (1) High correlations were found among sorption, denaturation and α-helix content and the random structure of hemoglobin. (2) The α-helix of hemoglobin is randomized as the surfactants denature hemoglobin. (3) Factorial analysis indicates that there are two factors involved in hemoglobin denaturation, one related to the destruction of the α-helix and the other related to a change in the β-structure. These two factors are related to change in the environment around the heme group. (4) Destruction of the α-helix seems to be one of the causes of the eye irritation produced by anionic surfactants.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.18.540</identifier><identifier>PMID: 7655423</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Biological and medical sciences ; Chromatography, High Pressure Liquid ; Circular Dichroism ; Drug toxicity and drugs side effects treatment ; eye irritation ; hemoglobin denaturation ; Hemoglobins - chemistry ; high-performance liquid chromatography ; Irritants - pharmacology ; Medical sciences ; Pharmacology. Drug treatments ; Protein Denaturation - drug effects ; Sodium Dodecyl Sulfate - pharmacology ; spectrophotometer ; Spectrophotometry ; Structure-Activity Relationship ; Surface-Active Agents - pharmacology ; surfactant ; Taurine - analogs & derivatives ; Taurine - pharmacology ; Toxicity: blood</subject><ispartof>Biological and Pharmaceutical Bulletin, 1995/04/15, Vol.18(4), pp.540-543</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1995 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1995</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c602t-251430b97dfac98ccb2255380cca783e7eee5213fbc4b6c6b62a7ba8720a50ee3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,4014,27914,27915,27916</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3567073$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7655423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HAYASHI, Toshikatsu</creatorcontrib><creatorcontrib>ITAGAKI, Hiroshi</creatorcontrib><creatorcontrib>FUKUDA, Toshio</creatorcontrib><creatorcontrib>TAMURA, Uhei</creatorcontrib><creatorcontrib>SATO, Yukio</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><title>Hemoglobin Denaturation Caused by Surfactants</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The properties of hemoglobin denaturation caused by two anionic surfactants (sodium dodecylsulfate and sodium lauroylmethyltaurate) were examined using spectrophotometry (multi-plate reader), circular dichroism (CD) and high-performance liquid chromatography (HPLC), and their denaturating action on hemoglobin and sorption to it were examined by various methods. Correlation and factorial analyses were applied to the experimental data and the following results were obtained : (1) High correlations were found among sorption, denaturation and α-helix content and the random structure of hemoglobin. (2) The α-helix of hemoglobin is randomized as the surfactants denature hemoglobin. (3) Factorial analysis indicates that there are two factors involved in hemoglobin denaturation, one related to the destruction of the α-helix and the other related to a change in the β-structure. These two factors are related to change in the environment around the heme group. (4) Destruction of the α-helix seems to be one of the causes of the eye irritation produced by anionic surfactants.</description><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Circular Dichroism</subject><subject>Drug toxicity and drugs side effects treatment</subject><subject>eye irritation</subject><subject>hemoglobin denaturation</subject><subject>Hemoglobins - chemistry</subject><subject>high-performance liquid chromatography</subject><subject>Irritants - pharmacology</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Protein Denaturation - drug effects</subject><subject>Sodium Dodecyl Sulfate - pharmacology</subject><subject>spectrophotometer</subject><subject>Spectrophotometry</subject><subject>Structure-Activity Relationship</subject><subject>Surface-Active Agents - pharmacology</subject><subject>surfactant</subject><subject>Taurine - analogs & derivatives</subject><subject>Taurine - pharmacology</subject><subject>Toxicity: blood</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1Lw0AQxRdRaq1evAsBPQmp-5ndHCV-VCh4UM_L7HZTU9Kk7iaH_vduSCgDM4f3m3nDQ-iW4CWhXD2Zg1kStRQcn6E5YVymghJxjuY4JyrNiFCX6CqEHcZYYspmaCYzIThlc5Su3L7d1q2pmuTFNdD1HrqqbZIC-uA2iTkmX70vwXbQdOEaXZRQB3czzQX6eXv9Llbp-vP9o3hepzbDtEupIJxhk8tNXMyVtYZSIZjC1oJUzEnnXPyQlcZyk9nMZBSkASUpBoGdYwt0P949-Pavd6HTu7b3TbTUhPOcZLFkpB5Hyvo2BO9KffDVHvxRE6yHYHQMRhOlYzARvptO9mbvNid0SiLqD5MOwUJdemhsFU4YE5nEcsCKEduFDrbupIPvKlu7wZHkORtc-dii-Um1v-C1a9g_RZGBWA</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>HAYASHI, Toshikatsu</creator><creator>ITAGAKI, Hiroshi</creator><creator>FUKUDA, Toshio</creator><creator>TAMURA, Uhei</creator><creator>SATO, Yukio</creator><creator>SUZUKI, Yasuo</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>1995</creationdate><title>Hemoglobin Denaturation Caused by Surfactants</title><author>HAYASHI, Toshikatsu ; ITAGAKI, Hiroshi ; FUKUDA, Toshio ; TAMURA, Uhei ; SATO, Yukio ; SUZUKI, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c602t-251430b97dfac98ccb2255380cca783e7eee5213fbc4b6c6b62a7ba8720a50ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Circular Dichroism</topic><topic>Drug toxicity and drugs side effects treatment</topic><topic>eye irritation</topic><topic>hemoglobin denaturation</topic><topic>Hemoglobins - chemistry</topic><topic>high-performance liquid chromatography</topic><topic>Irritants - pharmacology</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Protein Denaturation - drug effects</topic><topic>Sodium Dodecyl Sulfate - pharmacology</topic><topic>spectrophotometer</topic><topic>Spectrophotometry</topic><topic>Structure-Activity Relationship</topic><topic>Surface-Active Agents - pharmacology</topic><topic>surfactant</topic><topic>Taurine - analogs & derivatives</topic><topic>Taurine - pharmacology</topic><topic>Toxicity: blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HAYASHI, Toshikatsu</creatorcontrib><creatorcontrib>ITAGAKI, Hiroshi</creatorcontrib><creatorcontrib>FUKUDA, Toshio</creatorcontrib><creatorcontrib>TAMURA, Uhei</creatorcontrib><creatorcontrib>SATO, Yukio</creatorcontrib><creatorcontrib>SUZUKI, Yasuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HAYASHI, Toshikatsu</au><au>ITAGAKI, Hiroshi</au><au>FUKUDA, Toshio</au><au>TAMURA, Uhei</au><au>SATO, Yukio</au><au>SUZUKI, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hemoglobin Denaturation Caused by Surfactants</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1995</date><risdate>1995</risdate><volume>18</volume><issue>4</issue><spage>540</spage><epage>543</epage><pages>540-543</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The properties of hemoglobin denaturation caused by two anionic surfactants (sodium dodecylsulfate and sodium lauroylmethyltaurate) were examined using spectrophotometry (multi-plate reader), circular dichroism (CD) and high-performance liquid chromatography (HPLC), and their denaturating action on hemoglobin and sorption to it were examined by various methods. Correlation and factorial analyses were applied to the experimental data and the following results were obtained : (1) High correlations were found among sorption, denaturation and α-helix content and the random structure of hemoglobin. (2) The α-helix of hemoglobin is randomized as the surfactants denature hemoglobin. (3) Factorial analysis indicates that there are two factors involved in hemoglobin denaturation, one related to the destruction of the α-helix and the other related to a change in the β-structure. These two factors are related to change in the environment around the heme group. (4) Destruction of the α-helix seems to be one of the causes of the eye irritation produced by anionic surfactants.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>7655423</pmid><doi>10.1248/bpb.18.540</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Chromatography, High Pressure Liquid Circular Dichroism Drug toxicity and drugs side effects treatment eye irritation hemoglobin denaturation Hemoglobins - chemistry high-performance liquid chromatography Irritants - pharmacology Medical sciences Pharmacology. Drug treatments Protein Denaturation - drug effects Sodium Dodecyl Sulfate - pharmacology spectrophotometer Spectrophotometry Structure-Activity Relationship Surface-Active Agents - pharmacology surfactant Taurine - analogs & derivatives Taurine - pharmacology Toxicity: blood |
| title | Hemoglobin Denaturation Caused by Surfactants |
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