Separation of hydrophobic compounds by nonaqueous micellar electrokinetic chromatography using formamide solvent

In this study, some uncharged hydrophobic compounds, such as p-arylacetophenones, polycyclic aromatic hydrocarbons and steroids, were separated by micellar electrokinetic chromatography (MEKC) using amide solvents containing sodium n-dodecyl sulfate (SDS), di-(2-ethyl hexyl) sodium sulfosuccinate (AOT) or taurodexycholic acid sodium salt (STDC). The separation was shown to be dependent on the types of amide solvent and the surfactant concentration. Three different amides, formamide (FA), N-methyl formamide (NMF) and N, N-dimethylformamide (DMF) were used as solvent. A satisfactory result was obtained in formamide/SDS/NaH2PO4 medium. The effects of the amide solvent, surfactant and electrolyte on the separation were also studied.