Development of Fluorescence Lifetime Imaging Microscope Using Tunable Picosecond Laser for Application to Living Cells
A microscope was combined with a picosecond dye laser and an intensified charge-coupled-device camera. The resulting fluorescence lifetime imaging microscope (FLIM) was applied to biological cells, e.g., cancer cells. The present technique has sufficient sensitivity, and allowed us to measure a wide...
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| Veröffentlicht in: | BUNSEKI KAGAKU 2009, Vol.58(6), pp.487-494 |
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| container_end_page | 494 |
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| container_issue | 6 |
| container_start_page | 487 |
| container_title | BUNSEKI KAGAKU |
| container_volume | 58 |
| creator | Uchimura, Tomohiro Araki, Yoshie Kawanabe, Satoshi Miyake, Rina Imasaka, Totaro |
| description | A microscope was combined with a picosecond dye laser and an intensified charge-coupled-device camera. The resulting fluorescence lifetime imaging microscope (FLIM) was applied to biological cells, e.g., cancer cells. The present technique has sufficient sensitivity, and allowed us to measure a wide FLIM image within a few minutes. Therefore, the FLIM system developed in this study can be widely used to explain a variety of biological phenomena in living cells. |
| doi_str_mv | 10.2116/bunsekikagaku.58.487 |
| format | Article |
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| language | eng ; jpn |
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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | androgen receptor cancer cell fluorescence lifetime imaging microscopy picosecond laser red blood cell |
| title | Development of Fluorescence Lifetime Imaging Microscope Using Tunable Picosecond Laser for Application to Living Cells |
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