Micelle Enhanced Spectrofluorimetric Determination of L-ascorbic Acid Based on Laccase-linked Coupling Reaction Using Flow- injection Stopped-flow Technique
A novel spectrofluorimetric method for determination of L-ascorbic acid is described. It is based on the inhibition of L-ascorbic acid on the formation of 2, 3-diaminophenazine, which is an oxidation product of o-phenylenediamine catalyzed by laccase, in the presence of Brij-35, which shows strong e...
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| Veröffentlicht in: | Analytical Sciences 1997, Vol.13(Supplement), pp.67-70 |
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| container_issue | Supplement |
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| container_title | Analytical Sciences |
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| creator | Huang, Houping Cai, Ruxiu Korenaga, Takashi Zhang, Xinxiang Yang, Yi Du, Yumin Zeng, Yun'e |
| description | A novel spectrofluorimetric method for determination of L-ascorbic acid is described. It is based on the inhibition of L-ascorbic acid on the formation of 2, 3-diaminophenazine, which is an oxidation product of o-phenylenediamine catalyzed by laccase, in the presence of Brij-35, which shows strong enhancement on the fluorescence(at λem/λex=530nm/430nm) of the product, while no effect on the laccase-catalyzed reaction. The mechanism of o-phenylenediamine oxidation reaction catalyzed by laccase in the presence of L-ascorbic acid is discussed. L-ascorbic acid is determined in the range of O.02-2.Oμg/ml with a detection limit of 0.014μg/ml. The method is applied to the determination of L-ascorbic acid in pharmaceuticals, the results agree well with the nominal values and those obtained by the reference method. |
| doi_str_mv | 10.2116/analsci.13.Supplement_67 |
| format | Article |
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It is based on the inhibition of L-ascorbic acid on the formation of 2, 3-diaminophenazine, which is an oxidation product of o-phenylenediamine catalyzed by laccase, in the presence of Brij-35, which shows strong enhancement on the fluorescence(at λem/λex=530nm/430nm) of the product, while no effect on the laccase-catalyzed reaction. The mechanism of o-phenylenediamine oxidation reaction catalyzed by laccase in the presence of L-ascorbic acid is discussed. L-ascorbic acid is determined in the range of O.02-2.Oμg/ml with a detection limit of 0.014μg/ml. The method is applied to the determination of L-ascorbic acid in pharmaceuticals, the results agree well with the nominal values and those obtained by the reference method.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.13.Supplement_67</identifier><identifier>CODEN: ANSCEN</identifier><language>eng</language><publisher>Tokyo: The Japan Society for Analytical Chemistry</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Coenzymes, vitamins ; flow injection analysis ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; L-ascorbic acid ; laccase ; Medical sciences ; micelle ; Other biological molecules ; Pharmacology. Drug treatments ; spectrofluorimetry</subject><ispartof>Analytical Sciences, 1997, Vol.13(Supplement), pp.67-70</ispartof><rights>The Japan Society for Analytical Chemistry</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c612t-5e3ba9e8d0ba56afc76f9ab24ffeea692a9d74f4f47de998707a1a785eec44953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,781,785,790,791,1884,4025,4051,4052,23934,23935,25144,27927,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2299058$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Houping</creatorcontrib><creatorcontrib>Cai, Ruxiu</creatorcontrib><creatorcontrib>Korenaga, Takashi</creatorcontrib><creatorcontrib>Zhang, Xinxiang</creatorcontrib><creatorcontrib>Yang, Yi</creatorcontrib><creatorcontrib>Du, Yumin</creatorcontrib><creatorcontrib>Zeng, Yun'e</creatorcontrib><title>Micelle Enhanced Spectrofluorimetric Determination of L-ascorbic Acid Based on Laccase-linked Coupling Reaction Using Flow- injection Stopped-flow Technique</title><title>Analytical Sciences</title><description>A novel spectrofluorimetric method for determination of L-ascorbic acid is described. It is based on the inhibition of L-ascorbic acid on the formation of 2, 3-diaminophenazine, which is an oxidation product of o-phenylenediamine catalyzed by laccase, in the presence of Brij-35, which shows strong enhancement on the fluorescence(at λem/λex=530nm/430nm) of the product, while no effect on the laccase-catalyzed reaction. The mechanism of o-phenylenediamine oxidation reaction catalyzed by laccase in the presence of L-ascorbic acid is discussed. L-ascorbic acid is determined in the range of O.02-2.Oμg/ml with a detection limit of 0.014μg/ml. The method is applied to the determination of L-ascorbic acid in pharmaceuticals, the results agree well with the nominal values and those obtained by the reference method.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Coenzymes, vitamins</subject><subject>flow injection analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>L-ascorbic acid</subject><subject>laccase</subject><subject>Medical sciences</subject><subject>micelle</subject><subject>Other biological molecules</subject><subject>Pharmacology. 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Psychology</topic><topic>General pharmacology</topic><topic>L-ascorbic acid</topic><topic>laccase</topic><topic>Medical sciences</topic><topic>micelle</topic><topic>Other biological molecules</topic><topic>Pharmacology. 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It is based on the inhibition of L-ascorbic acid on the formation of 2, 3-diaminophenazine, which is an oxidation product of o-phenylenediamine catalyzed by laccase, in the presence of Brij-35, which shows strong enhancement on the fluorescence(at λem/λex=530nm/430nm) of the product, while no effect on the laccase-catalyzed reaction. The mechanism of o-phenylenediamine oxidation reaction catalyzed by laccase in the presence of L-ascorbic acid is discussed. L-ascorbic acid is determined in the range of O.02-2.Oμg/ml with a detection limit of 0.014μg/ml. The method is applied to the determination of L-ascorbic acid in pharmaceuticals, the results agree well with the nominal values and those obtained by the reference method.</abstract><cop>Tokyo</cop><pub>The Japan Society for Analytical Chemistry</pub><doi>10.2116/analsci.13.Supplement_67</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Coenzymes, vitamins flow injection analysis Fundamental and applied biological sciences. Psychology General pharmacology L-ascorbic acid laccase Medical sciences micelle Other biological molecules Pharmacology. Drug treatments spectrofluorimetry |
| title | Micelle Enhanced Spectrofluorimetric Determination of L-ascorbic Acid Based on Laccase-linked Coupling Reaction Using Flow- injection Stopped-flow Technique |
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