Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization
High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G20...
Gespeichert in:
| Veröffentlicht in: | Analytical Sciences 1993/02/10, Vol.9(1), pp.9-14 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 14 |
|---|---|
| container_issue | 1 |
| container_start_page | 9 |
| container_title | Analytical Sciences |
| container_volume | 9 |
| creator | ZHANG, Guo-Qing KAI, Masaaki NOPHTA, Hitoshi UMEGAE, Yoshihiko OHKURA, Yosuke |
| description | High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G2000SWXL) and converted into fluorescence derivatives by reaction with 4-methoxybenzamidine or meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium. The method required only 1μl of serum. The detection limits for glycated albumin and D-glucose (pmol on column) were 100 and 3 with the former reagent, and 150 and 5 with the latter, respectively. |
| doi_str_mv | 10.2116/analsci.9.9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1448512605</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3117764271</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-82d3bba861115552f7cb71e8462ea3608e6337f99dbaa4be77aa9cccc407640c3</originalsourceid><addsrcrecordid>eNo9kd2K2zAQhU1poem2V30BQXtXnEqyLFuXS9JNCoEubHttxvI4VrCtrH5a0rfpm1bZZHdADMw58zGaybKPjC45Y_IrzDB6bZZqqV5lC1aIOudcyNfZgipGc1kI-jZ75_2BUsZrzhfZvwczxTHAjDZ6ssaAbjIzBGNnYnuyGU8aAnbkdmxjEgjMHVnnmzFq65GkwjZOMJMHdHEi7YlszX7I79H11qW6RrIzj9F0ZDU4O0GwewfH4UT-mDCQe-uDtmOcZnI3RuvQazy3rNGZ32mEv09jvM_e9OlX-OGab7Jfd99-rrb57sfm--p2l2vBZMhr3hVtC7VkjJVlyftKtxXDWkiOUEhaoyyKqleqawFEi1UFoHQKQSspqC5usk8X7tHZx4g-NAcb3XmhDROiLhmXtEyuLxeXdtZ7h31zdGYCd2oYbc43aK43aFSjkvvzlQlew9i7tBLjX1qEVLJ6gq4utoMPsMcXHVwwesRnJFN1mbDs-p5VPYBrcC7-A4e1pIk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1448512605</pqid></control><display><type>article</type><title>Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization</title><source>J-STAGE Free</source><source>Freely Accessible Japanese Titles</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>ZHANG, Guo-Qing ; KAI, Masaaki ; NOPHTA, Hitoshi ; UMEGAE, Yoshihiko ; OHKURA, Yosuke</creator><creatorcontrib>ZHANG, Guo-Qing ; KAI, Masaaki ; NOPHTA, Hitoshi ; UMEGAE, Yoshihiko ; OHKURA, Yosuke</creatorcontrib><description>High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G2000SWXL) and converted into fluorescence derivatives by reaction with 4-methoxybenzamidine or meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium. The method required only 1μl of serum. The detection limits for glycated albumin and D-glucose (pmol on column) were 100 and 3 with the former reagent, and 150 and 5 with the latter, respectively.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.9.9</identifier><identifier>CODEN: ANSCEN</identifier><language>eng</language><publisher>Tokyo: The Japan Society for Analytical Chemistry</publisher><subject>4-methoxybenzamidine ; Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; D-glucose ; Fundamental and applied biological sciences. Psychology ; Glycated albumin ; high-performance liquid chromatography ; human serum ; meso-1,2-bis(4-methoxyphenyl)ethylenediamine ; postcolumn fluorescence derivatization ; size exclusion column</subject><ispartof>Analytical Sciences, 1993/02/10, Vol.9(1), pp.9-14</ispartof><rights>The Japan Society for Analytical Chemistry</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-82d3bba861115552f7cb71e8462ea3608e6337f99dbaa4be77aa9cccc407640c3</citedby><cites>FETCH-LOGICAL-c416t-82d3bba861115552f7cb71e8462ea3608e6337f99dbaa4be77aa9cccc407640c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4696705$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>ZHANG, Guo-Qing</creatorcontrib><creatorcontrib>KAI, Masaaki</creatorcontrib><creatorcontrib>NOPHTA, Hitoshi</creatorcontrib><creatorcontrib>UMEGAE, Yoshihiko</creatorcontrib><creatorcontrib>OHKURA, Yosuke</creatorcontrib><title>Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization</title><title>Analytical Sciences</title><description>High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G2000SWXL) and converted into fluorescence derivatives by reaction with 4-methoxybenzamidine or meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium. The method required only 1μl of serum. The detection limits for glycated albumin and D-glucose (pmol on column) were 100 and 3 with the former reagent, and 150 and 5 with the latter, respectively.</description><subject>4-methoxybenzamidine</subject><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>D-glucose</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycated albumin</subject><subject>high-performance liquid chromatography</subject><subject>human serum</subject><subject>meso-1,2-bis(4-methoxyphenyl)ethylenediamine</subject><subject>postcolumn fluorescence derivatization</subject><subject>size exclusion column</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNo9kd2K2zAQhU1poem2V30BQXtXnEqyLFuXS9JNCoEubHttxvI4VrCtrH5a0rfpm1bZZHdADMw58zGaybKPjC45Y_IrzDB6bZZqqV5lC1aIOudcyNfZgipGc1kI-jZ75_2BUsZrzhfZvwczxTHAjDZ6ssaAbjIzBGNnYnuyGU8aAnbkdmxjEgjMHVnnmzFq65GkwjZOMJMHdHEi7YlszX7I79H11qW6RrIzj9F0ZDU4O0GwewfH4UT-mDCQe-uDtmOcZnI3RuvQazy3rNGZ32mEv09jvM_e9OlX-OGab7Jfd99-rrb57sfm--p2l2vBZMhr3hVtC7VkjJVlyftKtxXDWkiOUEhaoyyKqleqawFEi1UFoHQKQSspqC5usk8X7tHZx4g-NAcb3XmhDROiLhmXtEyuLxeXdtZ7h31zdGYCd2oYbc43aK43aFSjkvvzlQlew9i7tBLjX1qEVLJ6gq4utoMPsMcXHVwwesRnJFN1mbDs-p5VPYBrcC7-A4e1pIk</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>ZHANG, Guo-Qing</creator><creator>KAI, Masaaki</creator><creator>NOPHTA, Hitoshi</creator><creator>UMEGAE, Yoshihiko</creator><creator>OHKURA, Yosuke</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>1993</creationdate><title>Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization</title><author>ZHANG, Guo-Qing ; KAI, Masaaki ; NOPHTA, Hitoshi ; UMEGAE, Yoshihiko ; OHKURA, Yosuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-82d3bba861115552f7cb71e8462ea3608e6337f99dbaa4be77aa9cccc407640c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>4-methoxybenzamidine</topic><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>D-glucose</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycated albumin</topic><topic>high-performance liquid chromatography</topic><topic>human serum</topic><topic>meso-1,2-bis(4-methoxyphenyl)ethylenediamine</topic><topic>postcolumn fluorescence derivatization</topic><topic>size exclusion column</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ZHANG, Guo-Qing</creatorcontrib><creatorcontrib>KAI, Masaaki</creatorcontrib><creatorcontrib>NOPHTA, Hitoshi</creatorcontrib><creatorcontrib>UMEGAE, Yoshihiko</creatorcontrib><creatorcontrib>OHKURA, Yosuke</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ZHANG, Guo-Qing</au><au>KAI, Masaaki</au><au>NOPHTA, Hitoshi</au><au>UMEGAE, Yoshihiko</au><au>OHKURA, Yosuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization</atitle><jtitle>Analytical Sciences</jtitle><date>1993</date><risdate>1993</risdate><volume>9</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><coden>ANSCEN</coden><abstract>High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G2000SWXL) and converted into fluorescence derivatives by reaction with 4-methoxybenzamidine or meso-1, 2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium. The method required only 1μl of serum. The detection limits for glycated albumin and D-glucose (pmol on column) were 100 and 3 with the former reagent, and 150 and 5 with the latter, respectively.</abstract><cop>Tokyo</cop><pub>The Japan Society for Analytical Chemistry</pub><doi>10.2116/analsci.9.9</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0910-6340 |
| ispartof | Analytical Sciences, 1993/02/10, Vol.9(1), pp.9-14 |
| issn | 0910-6340 1348-2246 |
| language | eng |
| recordid | cdi_proquest_journals_1448512605 |
| source | J-STAGE Free; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | 4-methoxybenzamidine Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences D-glucose Fundamental and applied biological sciences. Psychology Glycated albumin high-performance liquid chromatography human serum meso-1,2-bis(4-methoxyphenyl)ethylenediamine postcolumn fluorescence derivatization size exclusion column |
| title | Simultaneous Determination of Glycated Albumin and D-Glucose in Human Serum by High-Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T18%3A15%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simultaneous%20Determination%20of%20Glycated%20Albumin%20and%20D-Glucose%20in%20Human%20Serum%20by%20High-Performance%20Liquid%20Chromatography%20with%20Postcolumn%20Fluorescence%20Derivatization&rft.jtitle=Analytical%20Sciences&rft.au=ZHANG,%20Guo-Qing&rft.date=1993&rft.volume=9&rft.issue=1&rft.spage=9&rft.epage=14&rft.pages=9-14&rft.issn=0910-6340&rft.eissn=1348-2246&rft.coden=ANSCEN&rft_id=info:doi/10.2116/analsci.9.9&rft_dat=%3Cproquest_cross%3E3117764271%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1448512605&rft_id=info:pmid/&rfr_iscdi=true |