Determination of Reducing Sugars by High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization Using 1, 2-Bis(4-methoxyphenyl)ethylenediamine
A sensitive method for the determination of reducing sugars is described which employs high performance liquid chromatography with on-line postcolumn fluorescence derivatization. Reducing sugars are separated by borate complex anion-exchange chromatography on a trimethylammonium-bonded resin column...
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| Veröffentlicht in: | Analytical Sciences 1989/12/10, Vol.5(6), pp.675-680 |
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| container_end_page | 680 |
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| container_issue | 6 |
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| container_title | Analytical Sciences |
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| creator | UMEGAE, Yoshihiko NOHTA, Hitoshi OHKURA, Yosuke |
| description | A sensitive method for the determination of reducing sugars is described which employs high performance liquid chromatography with on-line postcolumn fluorescence derivatization. Reducing sugars are separated by borate complex anion-exchange chromatography on a trimethylammonium-bonded resin column (TSK gel Sugar AXG) and automatically converted into fluorescent derivatives by reaction with 1, 2-bis(4-methoxypheny)ethylenediamine in an alkaline medium. The detection limits (S/N=3) for reducing sugars are 30-160pmol in a 100-μl injection volume. The quantification of reducing sugars in human urine and serum is also described. |
| doi_str_mv | 10.2116/analsci.5.675 |
| format | Article |
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Reducing sugars are separated by borate complex anion-exchange chromatography on a trimethylammonium-bonded resin column (TSK gel Sugar AXG) and automatically converted into fluorescent derivatives by reaction with 1, 2-bis(4-methoxypheny)ethylenediamine in an alkaline medium. The detection limits (S/N=3) for reducing sugars are 30-160pmol in a 100-μl injection volume. The quantification of reducing sugars in human urine and serum is also described.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.5.675</identifier><identifier>CODEN: ANSCEN</identifier><language>eng</language><publisher>Tokyo: The Japan Society for Analytical Chemistry</publisher><subject>1,2-bis(4-methoxyphenyl)ethylenediamine ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; borate complex anion-exchange chromatography ; Carbohydrates ; Fundamental and applied biological sciences. 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Reducing sugars are separated by borate complex anion-exchange chromatography on a trimethylammonium-bonded resin column (TSK gel Sugar AXG) and automatically converted into fluorescent derivatives by reaction with 1, 2-bis(4-methoxypheny)ethylenediamine in an alkaline medium. The detection limits (S/N=3) for reducing sugars are 30-160pmol in a 100-μl injection volume. The quantification of reducing sugars in human urine and serum is also described.</description><subject>1,2-bis(4-methoxyphenyl)ethylenediamine</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>borate complex anion-exchange chromatography</subject><subject>Carbohydrates</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Other biological molecules</subject><subject>postcolumn fluorescence derivatization</subject><subject>Reducing sugar</subject><subject>serum</subject><subject>urine</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNo9kU9v1DAQxSNEJZaWI3dLcACpWWzH-eMj3bYUaSUQ0LPlOOPEqyTe2g6Qfp9-Txx2uwfLsuY3743nJclbgteUkOKTHGXvlVnn66LMXyQrkrEqpZQVL5MV5gSnRcbwq-S19zuMCa0oXSVP1xDADWaUwdgRWY1-QDMpM7bo59RK51E9ozvTdug7OG3dIEcFaGseJtOgTefsIINtndx3M_pjQsSsD8r20zCi236yDryCpeUanPkdTR4PRvd-sSCXiKZXxn9g6QChs3_nfQfj3H-Mj7mHERoj42xwkZzp-Dd4c7zPk_vbm1-bu3T77cvXzedtqhilIc1LwJQXdaFxluu6hjLjNaageA2kwZpTqnEJVZljLkuWc6BNRUE3uiYYVJadJ-8OuntnHybwQezs5Ja1CsJYxSrMKI9UeqCUs9470GLvzCDdLAgWSxLimITIRUwi8u-PqtIr2WsXd2j8qakoq4pyErGbA7bzQbZwqksXjOrhWZTwKl-E_58of6qrTjoBY_YP4vKnXA</recordid><startdate>19891201</startdate><enddate>19891201</enddate><creator>UMEGAE, Yoshihiko</creator><creator>NOHTA, Hitoshi</creator><creator>OHKURA, Yosuke</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>19891201</creationdate><title>Determination of Reducing Sugars by High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization Using 1, 2-Bis(4-methoxyphenyl)ethylenediamine</title><author>UMEGAE, Yoshihiko ; NOHTA, Hitoshi ; OHKURA, Yosuke</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-57e0296b6f035fbbe739b02ec9be1d0f922f07e87509a7459e2d82efdfb10ec33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>1,2-bis(4-methoxyphenyl)ethylenediamine</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>borate complex anion-exchange chromatography</topic><topic>Carbohydrates</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Other biological molecules</topic><topic>postcolumn fluorescence derivatization</topic><topic>Reducing sugar</topic><topic>serum</topic><topic>urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>UMEGAE, Yoshihiko</creatorcontrib><creatorcontrib>NOHTA, Hitoshi</creatorcontrib><creatorcontrib>OHKURA, Yosuke</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>UMEGAE, Yoshihiko</au><au>NOHTA, Hitoshi</au><au>OHKURA, Yosuke</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of Reducing Sugars by High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization Using 1, 2-Bis(4-methoxyphenyl)ethylenediamine</atitle><jtitle>Analytical Sciences</jtitle><date>1989-12-01</date><risdate>1989</risdate><volume>5</volume><issue>6</issue><spage>675</spage><epage>680</epage><pages>675-680</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><coden>ANSCEN</coden><abstract>A sensitive method for the determination of reducing sugars is described which employs high performance liquid chromatography with on-line postcolumn fluorescence derivatization. Reducing sugars are separated by borate complex anion-exchange chromatography on a trimethylammonium-bonded resin column (TSK gel Sugar AXG) and automatically converted into fluorescent derivatives by reaction with 1, 2-bis(4-methoxypheny)ethylenediamine in an alkaline medium. The detection limits (S/N=3) for reducing sugars are 30-160pmol in a 100-μl injection volume. The quantification of reducing sugars in human urine and serum is also described.</abstract><cop>Tokyo</cop><pub>The Japan Society for Analytical Chemistry</pub><doi>10.2116/analsci.5.675</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0910-6340 |
| ispartof | Analytical Sciences, 1989/12/10, Vol.5(6), pp.675-680 |
| issn | 0910-6340 1348-2246 |
| language | eng |
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| source | J-STAGE Free; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | 1,2-bis(4-methoxyphenyl)ethylenediamine Analytical, structural and metabolic biochemistry Biological and medical sciences borate complex anion-exchange chromatography Carbohydrates Fundamental and applied biological sciences. Psychology Other biological molecules postcolumn fluorescence derivatization Reducing sugar serum urine |
| title | Determination of Reducing Sugars by High Performance Liquid Chromatography with Postcolumn Fluorescence Derivatization Using 1, 2-Bis(4-methoxyphenyl)ethylenediamine |
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