Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography
A simultaneous fluorometric determination method of S-sulfocysteine and S-sulfoglutathione was established by HPLC using 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-F) as a prelabeling reagent. Chromatographic conditions were as follows: Column, TSK gel NH2-60 (150mm×4 mm i.d.); eluent, 0.1M citra...
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| Veröffentlicht in: | Analytical Sciences 1988/02/10, Vol.4(1), pp.101-104 |
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| creator | TOGAWA, Tadayasu KATO, Masahiro NACAI, Noriko IMANARI, Toshio |
| description | A simultaneous fluorometric determination method of S-sulfocysteine and S-sulfoglutathione was established by HPLC using 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-F) as a prelabeling reagent. Chromatographic conditions were as follows: Column, TSK gel NH2-60 (150mm×4 mm i.d.); eluent, 0.1M citrate buffer (pH 3.0) containing 10% methanol; flow rate, 1.0ml/min; sample size, 100μl; detection, Ex 475, Em 545nm. Calibration curves for S-sulfocysteine and S-sulfoglutathione were linear over the range of 0.1 to 10nmol. S-Sulfoglutathione was identified as an endogenous compound in rabbit red blood cells by the use of the present method. This method was also applied to the determination of S-sulfocysteine and S-sulfoglutathione in rabbit plasma and red blood cells after injection of sodium bisulfite. |
| doi_str_mv | 10.2116/analsci.4.101 |
| format | Article |
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Chromatographic conditions were as follows: Column, TSK gel NH2-60 (150mm×4 mm i.d.); eluent, 0.1M citrate buffer (pH 3.0) containing 10% methanol; flow rate, 1.0ml/min; sample size, 100μl; detection, Ex 475, Em 545nm. Calibration curves for S-sulfocysteine and S-sulfoglutathione were linear over the range of 0.1 to 10nmol. S-Sulfoglutathione was identified as an endogenous compound in rabbit red blood cells by the use of the present method. This method was also applied to the determination of S-sulfocysteine and S-sulfoglutathione in rabbit plasma and red blood cells after injection of sodium bisulfite.</description><identifier>ISSN: 0910-6340</identifier><identifier>EISSN: 1348-2246</identifier><identifier>DOI: 10.2116/analsci.4.101</identifier><language>eng</language><publisher>Tokyo: The Japan Society for Analytical Chemistry</publisher><subject>high performance liquid chromatography ; plasma ; red blood cells ; S-Sulfocysteine ; S-sulfoglutathione</subject><ispartof>Analytical Sciences, 1988/02/10, Vol.4(1), pp.101-104</ispartof><rights>The Japan Society for Analytical Chemistry</rights><rights>Copyright Japan Science and Technology Agency 1988</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-9869973e05ad8de2c811d026d9a57ac81a6a091dd7d824fea95ec57bb1c01cbb3</citedby><cites>FETCH-LOGICAL-c362t-9869973e05ad8de2c811d026d9a57ac81a6a091dd7d824fea95ec57bb1c01cbb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>TOGAWA, Tadayasu</creatorcontrib><creatorcontrib>KATO, Masahiro</creatorcontrib><creatorcontrib>NACAI, Noriko</creatorcontrib><creatorcontrib>IMANARI, Toshio</creatorcontrib><title>Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography</title><title>Analytical Sciences</title><description>A simultaneous fluorometric determination method of S-sulfocysteine and S-sulfoglutathione was established by HPLC using 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-F) as a prelabeling reagent. 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This method was also applied to the determination of S-sulfocysteine and S-sulfoglutathione in rabbit plasma and red blood cells after injection of sodium bisulfite.</description><subject>high performance liquid chromatography</subject><subject>plasma</subject><subject>red blood cells</subject><subject>S-Sulfocysteine</subject><subject>S-sulfoglutathione</subject><issn>0910-6340</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNo90M9PwyAUB3BiNHH-OHon8dwJlHbtUefPZImL0zN5hdeVpS0T6GEn_3U7px4I4fF5hPcl5IqzqeA8v4Ee2qDtVE4540dkwlNZJELI_JhMWMlZkqeSnZKzEDaMcVEIMSFf9xjRd7aHaF1PXU1XyWpoa6d3IaLtkUJv_mrrdogQmxEitT1dthA6-AFvaOhd65yhc2zbQKsdfbbrhi7R18530GukC_s52BE03nUQ3drDttldkJN6_DVe_u7n5OPx4X3-nCxen17mt4tEp7mISVnkZTlLkWVgCoNCF5wbJnJTQjaD8QQ5jCMaMzOFkDVCmaHOZlXFNeO6qtJzcn14d-vd54Ahqo0b_D4wxaUspMzHzEaVHJT2LgSPtdp624HfKc7UPmP1m7GS6uAfDn4TIqzxX4OPVrf4p3lZZPuOnzX2_d_rBrzCPv0G1W6Llg</recordid><startdate>19880101</startdate><enddate>19880101</enddate><creator>TOGAWA, Tadayasu</creator><creator>KATO, Masahiro</creator><creator>NACAI, Noriko</creator><creator>IMANARI, Toshio</creator><general>The Japan Society for Analytical Chemistry</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope></search><sort><creationdate>19880101</creationdate><title>Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography</title><author>TOGAWA, Tadayasu ; KATO, Masahiro ; NACAI, Noriko ; IMANARI, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-9869973e05ad8de2c811d026d9a57ac81a6a091dd7d824fea95ec57bb1c01cbb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>high performance liquid chromatography</topic><topic>plasma</topic><topic>red blood cells</topic><topic>S-Sulfocysteine</topic><topic>S-sulfoglutathione</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOGAWA, Tadayasu</creatorcontrib><creatorcontrib>KATO, Masahiro</creatorcontrib><creatorcontrib>NACAI, Noriko</creatorcontrib><creatorcontrib>IMANARI, Toshio</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOGAWA, Tadayasu</au><au>KATO, Masahiro</au><au>NACAI, Noriko</au><au>IMANARI, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography</atitle><jtitle>Analytical Sciences</jtitle><date>1988-01-01</date><risdate>1988</risdate><volume>4</volume><issue>1</issue><spage>101</spage><epage>104</epage><pages>101-104</pages><issn>0910-6340</issn><eissn>1348-2246</eissn><abstract>A simultaneous fluorometric determination method of S-sulfocysteine and S-sulfoglutathione was established by HPLC using 7-fluoro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-F) as a prelabeling reagent. Chromatographic conditions were as follows: Column, TSK gel NH2-60 (150mm×4 mm i.d.); eluent, 0.1M citrate buffer (pH 3.0) containing 10% methanol; flow rate, 1.0ml/min; sample size, 100μl; detection, Ex 475, Em 545nm. Calibration curves for S-sulfocysteine and S-sulfoglutathione were linear over the range of 0.1 to 10nmol. S-Sulfoglutathione was identified as an endogenous compound in rabbit red blood cells by the use of the present method. This method was also applied to the determination of S-sulfocysteine and S-sulfoglutathione in rabbit plasma and red blood cells after injection of sodium bisulfite.</abstract><cop>Tokyo</cop><pub>The Japan Society for Analytical Chemistry</pub><doi>10.2116/analsci.4.101</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Analytical Sciences, 1988/02/10, Vol.4(1), pp.101-104 |
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| language | eng |
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| source | J-STAGE Free; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | high performance liquid chromatography plasma red blood cells S-Sulfocysteine S-sulfoglutathione |
| title | Determination of S-Sulfocysteine and S-Sulfoglutathione in Plasma and Red Blood Cells by High Performance Liquid Chromatography |
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