Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean

A Japanese soybean variety, Kariyutaka, known to form many adventitious shoots from sections of the cotyledonary node, had a transformation frequency (relative number of T0 transgenic plants, which stably transmitted transgenes to their T1 progenies, to number of explants subjected to Agrobacterium-...

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Veröffentlicht in:Plant Biotechnology 2010/06/25, Vol.27(2), pp.217-220
Hauptverfasser: Yamada, Tetsuya, Watanabe, Satoshi, Arai, Maiko, Harada, Kyuya, Kitamura, Keisuke
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Sprache:eng
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