Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir
An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2012-12, Vol.48 (6), p.609-612 |
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| creator | Guo, Bin Stiles, Amanda R Liu, Chun-Zhao |
| description | An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of culture, 15.6 ± 1.4 shoots were regenerated per leaf explant on MS medium containing 0.5 μM TDZ. After transfer of shoots to a medium containing 5.0 μM indole-3-acetic acid, approximately 80% of the regenerated shoots formed roots and whole plantlets. After transfer of rooted shoots to the greenhouse, 83% of the regenerated plantlets survived and grew vigorously. The regeneration protocol developed in this study provides a basis for germplasm conservation and for the production of plant material necessary to study the medicinally active components of S. involucrata. |
| doi_str_mv | 10.1007/s11627-012-9468-6 |
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Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of culture, 15.6 ± 1.4 shoots were regenerated per leaf explant on MS medium containing 0.5 μM TDZ. After transfer of shoots to a medium containing 5.0 μM indole-3-acetic acid, approximately 80% of the regenerated shoots formed roots and whole plantlets. After transfer of rooted shoots to the greenhouse, 83% of the regenerated plantlets survived and grew vigorously. The regeneration protocol developed in this study provides a basis for germplasm conservation and for the production of plant material necessary to study the medicinally active components of S. involucrata.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-012-9468-6</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Acetic acid ; Biomedical and Life Sciences ; Cell Biology ; Chinese culture ; Developmental Biology ; Endangered plants ; Experiments ; Flowers & plants ; germplasm conservation ; Herbal medicine ; indole acetic acid ; Leaves ; Life Sciences ; Light ; Medicinal plants ; Methods ; MICROPROPAGATION ; Mortality ; Organogenesis ; Plant Breeding/Biotechnology ; Plant cells ; Plant Genetics and Genomics ; Plant propagation ; Plant roots ; Plant Sciences ; Plant tissues ; Plantlets ; Plants ; roots ; Saussurea ; Seedlings ; Seeds ; Shoots</subject><ispartof>In vitro cellular & developmental biology. Plant, 2012-12, Vol.48 (6), p.609-612</ispartof><rights>The Society for In Vitro Biology 2012</rights><rights>Copyright Society for In Vitro Biology Dec 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-59ee124ac56a06fc80c2c5e89dc967e19dede04c0ed5b3f457ce9419f5c8abdc3</citedby><cites>FETCH-LOGICAL-c386t-59ee124ac56a06fc80c2c5e89dc967e19dede04c0ed5b3f457ce9419f5c8abdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23326891$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23326891$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,27905,27906,41469,42538,51300,57998,58231</link.rule.ids></links><search><creatorcontrib>Guo, Bin</creatorcontrib><creatorcontrib>Stiles, Amanda R</creatorcontrib><creatorcontrib>Liu, Chun-Zhao</creatorcontrib><title>Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of culture, 15.6 ± 1.4 shoots were regenerated per leaf explant on MS medium containing 0.5 μM TDZ. After transfer of shoots to a medium containing 5.0 μM indole-3-acetic acid, approximately 80% of the regenerated shoots formed roots and whole plantlets. After transfer of rooted shoots to the greenhouse, 83% of the regenerated plantlets survived and grew vigorously. The regeneration protocol developed in this study provides a basis for germplasm conservation and for the production of plant material necessary to study the medicinally active components of S. involucrata.</description><subject>Acetic acid</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Chinese culture</subject><subject>Developmental Biology</subject><subject>Endangered plants</subject><subject>Experiments</subject><subject>Flowers & plants</subject><subject>germplasm conservation</subject><subject>Herbal medicine</subject><subject>indole acetic acid</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Medicinal plants</subject><subject>Methods</subject><subject>MICROPROPAGATION</subject><subject>Mortality</subject><subject>Organogenesis</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant cells</subject><subject>Plant Genetics and Genomics</subject><subject>Plant propagation</subject><subject>Plant roots</subject><subject>Plant Sciences</subject><subject>Plant tissues</subject><subject>Plantlets</subject><subject>Plants</subject><subject>roots</subject><subject>Saussurea</subject><subject>Seedlings</subject><subject>Seeds</subject><subject>Shoots</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1vFDEMhkcIJErhB3BAROI8Jc4kmckRVXxUrcSh7Tm4GWd3VttkiWdQ4deTZRDiAr7Ykt_Xth43zUuQZyBl_5YBrOpbCap12g6tfdScgO5Nq-zgHtdaGt0a3dunzTPmnZQSJPQnzZeb7TRO-GMpOQlKW0yBWPA251nkssGUN5SIJxax5HuxJ4yCHg57TDOLHMU1LsxLIRRT-pb3Syg4o7jEciZoFpdTed48ibhnevE7nza3H97fnH9qrz5_vDh_d9WGbrBzaxwRKI3BWJQ2hkEGFQwNbgzO9gRupJGkDpJGc9dFbfpAToOLJgx4N4butHmzzj2U_HUhnv0uLyXVlR601q4GmKqCVRVKZi4U_aFM91i-e5D-CNKvIH0F6Y8gva0etXq4atOGyl-T_2N6tZp2POfyZ4vquuNDoPZfr_2I2eOmTOxvr5UEXT9Tgfw69d8KJbvedj8BXNmTiA</recordid><startdate>20121201</startdate><enddate>20121201</enddate><creator>Guo, Bin</creator><creator>Stiles, Amanda R</creator><creator>Liu, Chun-Zhao</creator><general>Springer-Verlag</general><general>Springer Science + Business Media</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20121201</creationdate><title>Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir</title><author>Guo, Bin ; Stiles, Amanda R ; Liu, Chun-Zhao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-59ee124ac56a06fc80c2c5e89dc967e19dede04c0ed5b3f457ce9419f5c8abdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acetic acid</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Chinese culture</topic><topic>Developmental Biology</topic><topic>Endangered plants</topic><topic>Experiments</topic><topic>Flowers & plants</topic><topic>germplasm conservation</topic><topic>Herbal medicine</topic><topic>indole acetic acid</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Medicinal plants</topic><topic>Methods</topic><topic>MICROPROPAGATION</topic><topic>Mortality</topic><topic>Organogenesis</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant cells</topic><topic>Plant Genetics and Genomics</topic><topic>Plant propagation</topic><topic>Plant roots</topic><topic>Plant Sciences</topic><topic>Plant tissues</topic><topic>Plantlets</topic><topic>Plants</topic><topic>roots</topic><topic>Saussurea</topic><topic>Seedlings</topic><topic>Seeds</topic><topic>Shoots</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Bin</creatorcontrib><creatorcontrib>Stiles, Amanda R</creatorcontrib><creatorcontrib>Liu, Chun-Zhao</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Bin</au><au>Stiles, Amanda R</au><au>Liu, Chun-Zhao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2012-12-01</date><risdate>2012</risdate><volume>48</volume><issue>6</issue><spage>609</spage><epage>612</epage><pages>609-612</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>An efficient protocol for the in vitro micrpropagation of Saussurea involucrata Kar. et Kir, an endangered Chinese medicinal plant, was developed. Shoot organogenesis was obtained following culture of leaf explants on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ). After 28 d of culture, 15.6 ± 1.4 shoots were regenerated per leaf explant on MS medium containing 0.5 μM TDZ. After transfer of shoots to a medium containing 5.0 μM indole-3-acetic acid, approximately 80% of the regenerated shoots formed roots and whole plantlets. After transfer of rooted shoots to the greenhouse, 83% of the regenerated plantlets survived and grew vigorously. The regeneration protocol developed in this study provides a basis for germplasm conservation and for the production of plant material necessary to study the medicinally active components of S. involucrata.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s11627-012-9468-6</doi><tpages>4</tpages></addata></record> |
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| subjects | Acetic acid Biomedical and Life Sciences Cell Biology Chinese culture Developmental Biology Endangered plants Experiments Flowers & plants germplasm conservation Herbal medicine indole acetic acid Leaves Life Sciences Light Medicinal plants Methods MICROPROPAGATION Mortality Organogenesis Plant Breeding/Biotechnology Plant cells Plant Genetics and Genomics Plant propagation Plant roots Plant Sciences Plant tissues Plantlets Plants roots Saussurea Seedlings Seeds Shoots |
| title | Thidiazuron enhances shoot organogenesis from leaf explants of Saussurea involucrata Kar. et Kir |
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