Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light
An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of aux...
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description | An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and α-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 μM IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 μM IBA and 238 μM PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds. |
doi_str_mv | 10.1007/s11627-012-9486-4 |
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Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and α-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 μM IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 μM IBA and 238 μM PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-012-9486-4</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Acclimatization ; Acetic acid ; activated carbon ; Activated charcoal ; Adventitious roots ; Appropriate technology ; Auxins ; benzyladenine ; Biomedical and Life Sciences ; Callus ; callus formation ; Cell Biology ; Charcoal ; Culture media ; cytokinins ; Developmental Biology ; Experiments ; Growth conditions ; indole acetic acid ; indole butyric acid ; Jatropha curcas ; leaves ; Life Sciences ; Light emitting diodes ; Light quality ; Light sources ; naphthaleneacetic acid ; photoreceptors ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant propagation ; Plant roots ; Plant Sciences ; PLANT TISSUE CULTURE ; Plantlets ; Plants ; Production capacity ; Rooting ; Roots ; salt concentration ; Seeds ; Shoots ; stems ; Studies ; Woody plants ; zeatin</subject><ispartof>In vitro cellular & developmental biology. Plant, 2013-04, Vol.49 (2), p.183-190</ispartof><rights>2013 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2013</rights><rights>Copyright Society for In Vitro Biology Apr 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-f71be8160e29db4d8d763228973459bab8a67556649038c6058bb012701e1d663</citedby><cites>FETCH-LOGICAL-c386t-f71be8160e29db4d8d763228973459bab8a67556649038c6058bb012701e1d663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42568713$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42568713$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,41488,42557,51319,58017,58250</link.rule.ids></links><search><creatorcontrib>Daud, Norhayati</creatorcontrib><creatorcontrib>Faizal, Ahmad</creatorcontrib><creatorcontrib>Geelen, Danny</creatorcontrib><title>Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light</title><title>In vitro cellular & developmental biology. Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and α-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 μM IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 μM IBA and 238 μM PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds.</description><subject>Acclimatization</subject><subject>Acetic acid</subject><subject>activated carbon</subject><subject>Activated charcoal</subject><subject>Adventitious roots</subject><subject>Appropriate technology</subject><subject>Auxins</subject><subject>benzyladenine</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>callus formation</subject><subject>Cell Biology</subject><subject>Charcoal</subject><subject>Culture media</subject><subject>cytokinins</subject><subject>Developmental Biology</subject><subject>Experiments</subject><subject>Growth conditions</subject><subject>indole acetic acid</subject><subject>indole butyric acid</subject><subject>Jatropha curcas</subject><subject>leaves</subject><subject>Life Sciences</subject><subject>Light emitting diodes</subject><subject>Light quality</subject><subject>Light sources</subject><subject>naphthaleneacetic acid</subject><subject>photoreceptors</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant propagation</subject><subject>Plant roots</subject><subject>Plant Sciences</subject><subject>PLANT TISSUE CULTURE</subject><subject>Plantlets</subject><subject>Plants</subject><subject>Production capacity</subject><subject>Rooting</subject><subject>Roots</subject><subject>salt concentration</subject><subject>Seeds</subject><subject>Shoots</subject><subject>stems</subject><subject>Studies</subject><subject>Woody plants</subject><subject>zeatin</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UU1v1DAQjRCVKC0_gAPCEueUGWf8kWNV2gJaiQP0bDmJk_UqjRfbQeq_x0sQ4tTTjGbeezPzpqreIlwhgPqYECVXNSCvW9KyphfVOZISNZe6fVlyEFQLUvJV9TqlAwAgoDqvzPXwyy3ZZx_WxGII2S8TCyP7anMMx71l_Rp7m9juivnEUvaP62yzG1j3xI77OcQwzWvvlzAzu_ypxtLc3X5is5_2-bI6G-2c3Ju_8aJ6uLv9cfO53n27_3Jzvav7Rstcjwo7p1GC4-3Q0aAHJRvOdasaEm1nO22lEkJKaqHRvQShu64cqwAdDlI2F9WHTfcYw8_VpWwOYY1LGWmQiCSQ4E1B4YbqY0gputEco3-08ckgmJOPZvPRFGlz8tFQ4fCNkwp2mVz8T_kZ0ruNdEg5xH9TiAupFZ4Web_1RxuMnaJP5uE7B6TymGIIwbMIDgTY_AaN9o-b</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>Daud, Norhayati</creator><creator>Faizal, Ahmad</creator><creator>Geelen, Danny</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20130401</creationdate><title>Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light</title><author>Daud, Norhayati ; Faizal, Ahmad ; Geelen, Danny</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-f71be8160e29db4d8d763228973459bab8a67556649038c6058bb012701e1d663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acclimatization</topic><topic>Acetic acid</topic><topic>activated carbon</topic><topic>Activated charcoal</topic><topic>Adventitious roots</topic><topic>Appropriate technology</topic><topic>Auxins</topic><topic>benzyladenine</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>callus formation</topic><topic>Cell Biology</topic><topic>Charcoal</topic><topic>Culture media</topic><topic>cytokinins</topic><topic>Developmental Biology</topic><topic>Experiments</topic><topic>Growth conditions</topic><topic>indole acetic acid</topic><topic>indole butyric acid</topic><topic>Jatropha curcas</topic><topic>leaves</topic><topic>Life Sciences</topic><topic>Light emitting diodes</topic><topic>Light quality</topic><topic>Light sources</topic><topic>naphthaleneacetic acid</topic><topic>photoreceptors</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant propagation</topic><topic>Plant roots</topic><topic>Plant Sciences</topic><topic>PLANT TISSUE CULTURE</topic><topic>Plantlets</topic><topic>Plants</topic><topic>Production capacity</topic><topic>Rooting</topic><topic>Roots</topic><topic>salt concentration</topic><topic>Seeds</topic><topic>Shoots</topic><topic>stems</topic><topic>Studies</topic><topic>Woody plants</topic><topic>zeatin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Daud, Norhayati</creatorcontrib><creatorcontrib>Faizal, Ahmad</creatorcontrib><creatorcontrib>Geelen, Danny</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Daud, Norhayati</au><au>Faizal, Ahmad</au><au>Geelen, Danny</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2013-04-01</date><risdate>2013</risdate><volume>49</volume><issue>2</issue><spage>183</spage><epage>190</epage><pages>183-190</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and α-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 μM IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 μM IBA and 238 μM PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><doi>10.1007/s11627-012-9486-4</doi><tpages>8</tpages></addata></record> |
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subjects | Acclimatization Acetic acid activated carbon Activated charcoal Adventitious roots Appropriate technology Auxins benzyladenine Biomedical and Life Sciences Callus callus formation Cell Biology Charcoal Culture media cytokinins Developmental Biology Experiments Growth conditions indole acetic acid indole butyric acid Jatropha curcas leaves Life Sciences Light emitting diodes Light quality Light sources naphthaleneacetic acid photoreceptors Plant Breeding/Biotechnology Plant Genetics and Genomics Plant propagation Plant roots Plant Sciences PLANT TISSUE CULTURE Plantlets Plants Production capacity Rooting Roots salt concentration Seeds Shoots stems Studies Woody plants zeatin |
title | Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light |
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