Endotoxin potentiates lung injury in cerulein-induced pancreatitis
In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP). Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours aft...
Gespeichert in:
| Veröffentlicht in: | The American journal of surgery 2003-11, Vol.186 (5), p.526-530 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 530 |
|---|---|
| container_issue | 5 |
| container_start_page | 526 |
| container_title | The American journal of surgery |
| container_volume | 186 |
| creator | Gray, Keith D Simovic, Misho O Chapman, William C Blackwell, Timothy S Christman, John W May, Addison K Parman, Kelly S Stain, Steven C |
| description | In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP).
Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours after the initial cerulein injection. Twenty-four hours after LPS injection, myeloperoxidase (MPO) activity, nuclear factor (NF)-κB activation, and tumor necrosis factor (TNF)-α, interleukin (IL)-6, and chemokines MIP-2 and KC levels were measured in pancreas, liver, and lung tissues. Four groups of mice were studied: cerulein-LPS, cerulein-saline, saline-LPS, and saline-saline treated mice.
Elevated serum lipase confirmed pancreatitis in cerulein treated mice. Lung MPO activity was significantly increased in the cerulein-LPS group. NF-κB was activated in the liver but not in pancreas and lung tissue. Chemokines MIP-2 and KC were elevated in pancreatic tissue only.
These findings suggest that gram-negative infections may be an important predisposition for the development of adult respiratory distress syndrome in the setting of AP and that hepatic NF-κB may mediate multisystem injury. |
| doi_str_mv | 10.1016/j.amjsurg.2003.07.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1444601032</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0002961003003337</els_id><sourcerecordid>3107725441</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-c76116459c5512a4cb0b4bbac368ccec2ff8f18c8ced4cb203b6a19c1113e1a43</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMotlZ_grIgHnfNbLJfJ9FSP6DgRc8hO5stWdpsTbJi_70pXehNT0OSJ-_MPIRcA02AQn7fJXLTucGukpRSltAioUBPyBTKooqhLNkpmVJK07jKgU7IhXNdOAJwdk4mwLMq3FdT8rQwTe_7H22ibe-V8Vp65aL1YFaRNt1gd6FEqOywVtrE2jQDqibaSoNWSa-9dpfkrJVrp67GOiOfz4uP-Wu8fH95mz8uY-S88DEWOUAeGmOWQSo51rTmdS2R5SWiwrRtyxZKLEN-eEwpq3MJFQIAUyA5m5HbQ-7W9l-Dcl50_WBNaCmAc56H_Vn6J0UZQFnxjAUqO1Boe-esasXW6o20uwCJvV_RidGv2PsVtBD7_Bm5GdOHeqOa469RaADuRkA6lOvWBlHaHbksZRVj-6CHA6eCsW-trHColQm7a6vQi6bX_4zyC7VHmy0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1031189453</pqid></control><display><type>article</type><title>Endotoxin potentiates lung injury in cerulein-induced pancreatitis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Gray, Keith D ; Simovic, Misho O ; Chapman, William C ; Blackwell, Timothy S ; Christman, John W ; May, Addison K ; Parman, Kelly S ; Stain, Steven C</creator><creatorcontrib>Gray, Keith D ; Simovic, Misho O ; Chapman, William C ; Blackwell, Timothy S ; Christman, John W ; May, Addison K ; Parman, Kelly S ; Stain, Steven C</creatorcontrib><description>In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP).
Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours after the initial cerulein injection. Twenty-four hours after LPS injection, myeloperoxidase (MPO) activity, nuclear factor (NF)-κB activation, and tumor necrosis factor (TNF)-α, interleukin (IL)-6, and chemokines MIP-2 and KC levels were measured in pancreas, liver, and lung tissues. Four groups of mice were studied: cerulein-LPS, cerulein-saline, saline-LPS, and saline-saline treated mice.
Elevated serum lipase confirmed pancreatitis in cerulein treated mice. Lung MPO activity was significantly increased in the cerulein-LPS group. NF-κB was activated in the liver but not in pancreas and lung tissue. Chemokines MIP-2 and KC were elevated in pancreatic tissue only.
These findings suggest that gram-negative infections may be an important predisposition for the development of adult respiratory distress syndrome in the setting of AP and that hepatic NF-κB may mediate multisystem injury.</description><identifier>ISSN: 0002-9610</identifier><identifier>EISSN: 1879-1883</identifier><identifier>DOI: 10.1016/j.amjsurg.2003.07.010</identifier><identifier>PMID: 14599619</identifier><identifier>CODEN: AJSUAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Bacterial infections ; Biological and medical sciences ; Ceruletide ; Chemokines ; Cytokines - metabolism ; Endotoxin ; Endotoxins - pharmacology ; Enzymes ; Gastroenterology. Liver. Pancreas. Abdomen ; Injuries of the thorax. Foreign bodies. Diseases due to physical agents ; Laboratory animals ; Lipopolysaccharide ; Liver - metabolism ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Lung injury ; Lungs ; Male ; Medical sciences ; Mice ; Mice, Inbred C57BL ; NF-kappa B - metabolism ; Nuclear factor-κB ; Other diseases. Semiology ; Pancreas ; Pancreatitis ; Pancreatitis - chemically induced ; Peroxidase - metabolism ; Potassium ; Proteins ; Respiratory distress syndrome ; Respiratory Distress Syndrome, Adult - microbiology ; Rodents ; Traumas. Diseases due to physical agents</subject><ispartof>The American journal of surgery, 2003-11, Vol.186 (5), p.526-530</ispartof><rights>2003 Elsevier Inc.</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Elsevier Limited Jan 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-c76116459c5512a4cb0b4bbac368ccec2ff8f18c8ced4cb203b6a19c1113e1a43</citedby><cites>FETCH-LOGICAL-c447t-c76116459c5512a4cb0b4bbac368ccec2ff8f18c8ced4cb203b6a19c1113e1a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002961003003337$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15239330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14599619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gray, Keith D</creatorcontrib><creatorcontrib>Simovic, Misho O</creatorcontrib><creatorcontrib>Chapman, William C</creatorcontrib><creatorcontrib>Blackwell, Timothy S</creatorcontrib><creatorcontrib>Christman, John W</creatorcontrib><creatorcontrib>May, Addison K</creatorcontrib><creatorcontrib>Parman, Kelly S</creatorcontrib><creatorcontrib>Stain, Steven C</creatorcontrib><title>Endotoxin potentiates lung injury in cerulein-induced pancreatitis</title><title>The American journal of surgery</title><addtitle>Am J Surg</addtitle><description>In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP).
Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours after the initial cerulein injection. Twenty-four hours after LPS injection, myeloperoxidase (MPO) activity, nuclear factor (NF)-κB activation, and tumor necrosis factor (TNF)-α, interleukin (IL)-6, and chemokines MIP-2 and KC levels were measured in pancreas, liver, and lung tissues. Four groups of mice were studied: cerulein-LPS, cerulein-saline, saline-LPS, and saline-saline treated mice.
Elevated serum lipase confirmed pancreatitis in cerulein treated mice. Lung MPO activity was significantly increased in the cerulein-LPS group. NF-κB was activated in the liver but not in pancreas and lung tissue. Chemokines MIP-2 and KC were elevated in pancreatic tissue only.
These findings suggest that gram-negative infections may be an important predisposition for the development of adult respiratory distress syndrome in the setting of AP and that hepatic NF-κB may mediate multisystem injury.</description><subject>Animals</subject><subject>Bacterial infections</subject><subject>Biological and medical sciences</subject><subject>Ceruletide</subject><subject>Chemokines</subject><subject>Cytokines - metabolism</subject><subject>Endotoxin</subject><subject>Endotoxins - pharmacology</subject><subject>Enzymes</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Injuries of the thorax. Foreign bodies. Diseases due to physical agents</subject><subject>Laboratory animals</subject><subject>Lipopolysaccharide</subject><subject>Liver - metabolism</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Lung injury</subject><subject>Lungs</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>NF-kappa B - metabolism</subject><subject>Nuclear factor-κB</subject><subject>Other diseases. Semiology</subject><subject>Pancreas</subject><subject>Pancreatitis</subject><subject>Pancreatitis - chemically induced</subject><subject>Peroxidase - metabolism</subject><subject>Potassium</subject><subject>Proteins</subject><subject>Respiratory distress syndrome</subject><subject>Respiratory Distress Syndrome, Adult - microbiology</subject><subject>Rodents</subject><subject>Traumas. Diseases due to physical agents</subject><issn>0002-9610</issn><issn>1879-1883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkE1LAzEQhoMotlZ_grIgHnfNbLJfJ9FSP6DgRc8hO5stWdpsTbJi_70pXehNT0OSJ-_MPIRcA02AQn7fJXLTucGukpRSltAioUBPyBTKooqhLNkpmVJK07jKgU7IhXNdOAJwdk4mwLMq3FdT8rQwTe_7H22ibe-V8Vp65aL1YFaRNt1gd6FEqOywVtrE2jQDqibaSoNWSa-9dpfkrJVrp67GOiOfz4uP-Wu8fH95mz8uY-S88DEWOUAeGmOWQSo51rTmdS2R5SWiwrRtyxZKLEN-eEwpq3MJFQIAUyA5m5HbQ-7W9l-Dcl50_WBNaCmAc56H_Vn6J0UZQFnxjAUqO1Boe-esasXW6o20uwCJvV_RidGv2PsVtBD7_Bm5GdOHeqOa469RaADuRkA6lOvWBlHaHbksZRVj-6CHA6eCsW-trHColQm7a6vQi6bX_4zyC7VHmy0</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Gray, Keith D</creator><creator>Simovic, Misho O</creator><creator>Chapman, William C</creator><creator>Blackwell, Timothy S</creator><creator>Christman, John W</creator><creator>May, Addison K</creator><creator>Parman, Kelly S</creator><creator>Stain, Steven C</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20031101</creationdate><title>Endotoxin potentiates lung injury in cerulein-induced pancreatitis</title><author>Gray, Keith D ; Simovic, Misho O ; Chapman, William C ; Blackwell, Timothy S ; Christman, John W ; May, Addison K ; Parman, Kelly S ; Stain, Steven C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-c76116459c5512a4cb0b4bbac368ccec2ff8f18c8ced4cb203b6a19c1113e1a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacterial infections</topic><topic>Biological and medical sciences</topic><topic>Ceruletide</topic><topic>Chemokines</topic><topic>Cytokines - metabolism</topic><topic>Endotoxin</topic><topic>Endotoxins - pharmacology</topic><topic>Enzymes</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Injuries of the thorax. Foreign bodies. Diseases due to physical agents</topic><topic>Laboratory animals</topic><topic>Lipopolysaccharide</topic><topic>Liver - metabolism</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Lung injury</topic><topic>Lungs</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>NF-kappa B - metabolism</topic><topic>Nuclear factor-κB</topic><topic>Other diseases. Semiology</topic><topic>Pancreas</topic><topic>Pancreatitis</topic><topic>Pancreatitis - chemically induced</topic><topic>Peroxidase - metabolism</topic><topic>Potassium</topic><topic>Proteins</topic><topic>Respiratory distress syndrome</topic><topic>Respiratory Distress Syndrome, Adult - microbiology</topic><topic>Rodents</topic><topic>Traumas. Diseases due to physical agents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gray, Keith D</creatorcontrib><creatorcontrib>Simovic, Misho O</creatorcontrib><creatorcontrib>Chapman, William C</creatorcontrib><creatorcontrib>Blackwell, Timothy S</creatorcontrib><creatorcontrib>Christman, John W</creatorcontrib><creatorcontrib>May, Addison K</creatorcontrib><creatorcontrib>Parman, Kelly S</creatorcontrib><creatorcontrib>Stain, Steven C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>The American journal of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gray, Keith D</au><au>Simovic, Misho O</au><au>Chapman, William C</au><au>Blackwell, Timothy S</au><au>Christman, John W</au><au>May, Addison K</au><au>Parman, Kelly S</au><au>Stain, Steven C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endotoxin potentiates lung injury in cerulein-induced pancreatitis</atitle><jtitle>The American journal of surgery</jtitle><addtitle>Am J Surg</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>186</volume><issue>5</issue><spage>526</spage><epage>530</epage><pages>526-530</pages><issn>0002-9610</issn><eissn>1879-1883</eissn><coden>AJSUAB</coden><abstract>In this study we examine the effect of endotoxin (lipopolysaccharide) on lung injury in the setting of acute pancreatitis (AP).
Twelve hourly injections of cerulein (50 μg/kg/h) were used to induce pancreatitis in mice. Intraperitoneal lipopolysaccharide (LPS [6 mg/kg]) was administered 24 hours after the initial cerulein injection. Twenty-four hours after LPS injection, myeloperoxidase (MPO) activity, nuclear factor (NF)-κB activation, and tumor necrosis factor (TNF)-α, interleukin (IL)-6, and chemokines MIP-2 and KC levels were measured in pancreas, liver, and lung tissues. Four groups of mice were studied: cerulein-LPS, cerulein-saline, saline-LPS, and saline-saline treated mice.
Elevated serum lipase confirmed pancreatitis in cerulein treated mice. Lung MPO activity was significantly increased in the cerulein-LPS group. NF-κB was activated in the liver but not in pancreas and lung tissue. Chemokines MIP-2 and KC were elevated in pancreatic tissue only.
These findings suggest that gram-negative infections may be an important predisposition for the development of adult respiratory distress syndrome in the setting of AP and that hepatic NF-κB may mediate multisystem injury.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14599619</pmid><doi>10.1016/j.amjsurg.2003.07.010</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-9610 |
| ispartof | The American journal of surgery, 2003-11, Vol.186 (5), p.526-530 |
| issn | 0002-9610 1879-1883 |
| language | eng |
| recordid | cdi_proquest_journals_1444601032 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacterial infections Biological and medical sciences Ceruletide Chemokines Cytokines - metabolism Endotoxin Endotoxins - pharmacology Enzymes Gastroenterology. Liver. Pancreas. Abdomen Injuries of the thorax. Foreign bodies. Diseases due to physical agents Laboratory animals Lipopolysaccharide Liver - metabolism Liver. Biliary tract. Portal circulation. Exocrine pancreas Lung injury Lungs Male Medical sciences Mice Mice, Inbred C57BL NF-kappa B - metabolism Nuclear factor-κB Other diseases. Semiology Pancreas Pancreatitis Pancreatitis - chemically induced Peroxidase - metabolism Potassium Proteins Respiratory distress syndrome Respiratory Distress Syndrome, Adult - microbiology Rodents Traumas. Diseases due to physical agents |
| title | Endotoxin potentiates lung injury in cerulein-induced pancreatitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T22%3A19%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Endotoxin%20potentiates%20lung%20injury%20in%20cerulein-induced%20pancreatitis&rft.jtitle=The%20American%20journal%20of%20surgery&rft.au=Gray,%20Keith%20D&rft.date=2003-11-01&rft.volume=186&rft.issue=5&rft.spage=526&rft.epage=530&rft.pages=526-530&rft.issn=0002-9610&rft.eissn=1879-1883&rft.coden=AJSUAB&rft_id=info:doi/10.1016/j.amjsurg.2003.07.010&rft_dat=%3Cproquest_cross%3E3107725441%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1031189453&rft_id=info:pmid/14599619&rft_els_id=S0002961003003337&rfr_iscdi=true |