Propagation of the Sperm Genome
Among infertile men the degree of spermatogenesis varies from a barely compromised sperm number in the ejaculate to complete absence-the condition known as azoospermia. The scarcity of gametes often represents a major hindrance to overcoming spermatogenic failure through the use of assisted fertiliz...
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| Veröffentlicht in: | Journal of Mammalian Ova Research 2009-10, Vol.26 (4), p.202-206 |
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| container_title | Journal of Mammalian Ova Research |
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| creator | Takeuchi, Takumi Watanabe, Noriko Yoshida, Atsumi Palermo, Gianpiero D. |
| description | Among infertile men the degree of spermatogenesis varies from a barely compromised sperm number in the ejaculate to complete absence-the condition known as azoospermia. The scarcity of gametes often represents a major hindrance to overcoming spermatogenic failure through the use of assisted fertilization techniques. Therefore, we attempted to replicate the fertilizing spermatozoon by ‘male genome cloning’ in order to empower an individual spermatozoon. Spermatozoa injected into mature ooplasts were capable of maintaining and replicating a haploid male genome as haploid androgenotes. Isolated nuclei from such androgenotes entered into syngamy with female PNs following nuclear transfer fertilization. By utilizing mouse 8-cell stage androgenotes, we were able to obtain 6 blastocysts per single spermatozoon, and consequently live offspring. Thus, we were able to enhance the reproductive performance of single spermatozoa, indicating a possible future application of male gamete genome profiling prior to fertilization. |
| doi_str_mv | 10.1274/jmor.26.202 |
| format | Article |
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| source | BioOne Complete; EZB-FREE-00999 freely available EZB journals; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | ANIMAL ANIMALES ANIMALS CLONACION MOLECULAR CLONAGE MOLECULAIRE DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Embryonic stem cell ESPERMATOGENESIS ESPERMATOZOO GAMETOGENESE GAMETOGENESIS GENERO HUMANO GENOMAS GENOME GENOMES GENRE HUMAIN http://www.fao.org/aos/agrovoc#c_2238 http://www.fao.org/aos/agrovoc#c_27503 http://www.fao.org/aos/agrovoc#c_27931 http://www.fao.org/aos/agrovoc#c_3224 http://www.fao.org/aos/agrovoc#c_444 http://www.fao.org/aos/agrovoc#c_4586 http://www.fao.org/aos/agrovoc#c_7285 http://www.fao.org/aos/agrovoc#c_7287 Male genome cloning MANKIND MOLECULAR CLONING Prefertilization genetic diagnosis SPERMATOGENESE SPERMATOGENESIS SPERMATOZOA SPERMATOZOIDE |
| title | Propagation of the Sperm Genome |
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