Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes
Bovine oocytes were matured and fertilized in a medium supplemented with or without 0.1% linoleic acid-albumin (LAA). The presumptive pronuclear-stage zygotes were frozen-thawed in the medium containing 1.5 M ethylene glycol with or without 0.1 M sucrose. The post-thaw survival of the zygotes was as...
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| Veröffentlicht in: | Journal of mammalian ova research 1999, Vol.16 (1), p.19-22 |
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| container_title | Journal of mammalian ova research |
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| creator | Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science) Kanamori, A Sugisawa, K Kimura, K Hanada, A |
| description | Bovine oocytes were matured and fertilized in a medium supplemented with or without 0.1% linoleic acid-albumin (LAA). The presumptive pronuclear-stage zygotes were frozen-thawed in the medium containing 1.5 M ethylene glycol with or without 0.1 M sucrose. The post-thaw survival of the zygotes was assessed by blastocyst development in vitro. In non-frozen control groups, 66 vs 64% (p=0.757) of the zygotes that were produced in the presence vs absence of LAA cleaved and 25 vs 27% (p=0.883) developed to blastocysts, respectively. After freezing and thawing, similar proportions (53-65%) of the zygotes appeared to be normal regardless of the LAA treatment in the IVM / IVF media (P≥0.775) and sucrose inclusion in the cryoprotective medium (P≥0.177). However, the post-thaw cleavage rate of LAA-treated zygotes (45-54%) was significantly higher (p |
| doi_str_mv | 10.1274/jmor.16.19 |
| format | Article |
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(Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science) ; Kanamori, A ; Sugisawa, K ; Kimura, K ; Hanada, A</creator><creatorcontrib>Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science) ; Kanamori, A ; Sugisawa, K ; Kimura, K ; Hanada, A</creatorcontrib><description>Bovine oocytes were matured and fertilized in a medium supplemented with or without 0.1% linoleic acid-albumin (LAA). The presumptive pronuclear-stage zygotes were frozen-thawed in the medium containing 1.5 M ethylene glycol with or without 0.1 M sucrose. The post-thaw survival of the zygotes was assessed by blastocyst development in vitro. In non-frozen control groups, 66 vs 64% (p=0.757) of the zygotes that were produced in the presence vs absence of LAA cleaved and 25 vs 27% (p=0.883) developed to blastocysts, respectively. After freezing and thawing, similar proportions (53-65%) of the zygotes appeared to be normal regardless of the LAA treatment in the IVM / IVF media (P≥0.775) and sucrose inclusion in the cryoprotective medium (P≥0.177). However, the post-thaw cleavage rate of LAA-treated zygotes (45-54%) was significantly higher (p<0.008) than that of non LAA-treated zygotes (23-29%). The post-thaw development of LAA-treated zygotes into blastocysts (14%) was also higher (p<0.003) than that of non LAA-treated zygotes (2%). Developmental kinetics and cell numbers of the resultant blastocysts were similar between frozen and non-frozen groups. These results suggest that presumptive pronuclear-stage bovine zygotes matured and fertilized in the presence of LAA are relatively tolerant to the process of freezing and thawing, although the post-thaw survival rate needs to be further improved.</description><identifier>ISSN: 1341-7738</identifier><identifier>EISSN: 1347-5878</identifier><identifier>DOI: 10.1274/jmor.16.19</identifier><language>eng</language><publisher>Fujisawa: Japan Science and Technology Agency</publisher><subject>ACIDE LINOLEIQUE ; ACIDO LINOLEICO ; ALBUMINAS ; ALBUMINE ; ALBUMINS ; BOVIN ; CATTLE ; CIGOTOS ; CONSERVACION DE EMBRIONES ; CONSERVATION D'EMBRYON ; CULTURE MEDIA ; EMBRYO PRESERVATION ; GANADO BOVINO ; LINOLEIC ACID ; MEDIO DE CULTIVO ; MILIEU DE CULTURE ; SUPERVIVENCIA ; SURVIE ; SURVIVAL ; ZYGOTE ; ZYGOTES</subject><ispartof>Journal of mammalian ova research, 1999, Vol.16 (1), p.19-22</ispartof><rights>Copyright Japan Science and Technology Agency 1999</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4229-36b296747265aacacd88c14f293c65b30b0a6f94d4cb963a641a380e52c21f9d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids></links><search><creatorcontrib>Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science)</creatorcontrib><creatorcontrib>Kanamori, A</creatorcontrib><creatorcontrib>Sugisawa, K</creatorcontrib><creatorcontrib>Kimura, K</creatorcontrib><creatorcontrib>Hanada, A</creatorcontrib><title>Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes</title><title>Journal of mammalian ova research</title><description>Bovine oocytes were matured and fertilized in a medium supplemented with or without 0.1% linoleic acid-albumin (LAA). The presumptive pronuclear-stage zygotes were frozen-thawed in the medium containing 1.5 M ethylene glycol with or without 0.1 M sucrose. The post-thaw survival of the zygotes was assessed by blastocyst development in vitro. In non-frozen control groups, 66 vs 64% (p=0.757) of the zygotes that were produced in the presence vs absence of LAA cleaved and 25 vs 27% (p=0.883) developed to blastocysts, respectively. After freezing and thawing, similar proportions (53-65%) of the zygotes appeared to be normal regardless of the LAA treatment in the IVM / IVF media (P≥0.775) and sucrose inclusion in the cryoprotective medium (P≥0.177). However, the post-thaw cleavage rate of LAA-treated zygotes (45-54%) was significantly higher (p<0.008) than that of non LAA-treated zygotes (23-29%). The post-thaw development of LAA-treated zygotes into blastocysts (14%) was also higher (p<0.003) than that of non LAA-treated zygotes (2%). Developmental kinetics and cell numbers of the resultant blastocysts were similar between frozen and non-frozen groups. These results suggest that presumptive pronuclear-stage bovine zygotes matured and fertilized in the presence of LAA are relatively tolerant to the process of freezing and thawing, although the post-thaw survival rate needs to be further improved.</description><subject>ACIDE LINOLEIQUE</subject><subject>ACIDO LINOLEICO</subject><subject>ALBUMINAS</subject><subject>ALBUMINE</subject><subject>ALBUMINS</subject><subject>BOVIN</subject><subject>CATTLE</subject><subject>CIGOTOS</subject><subject>CONSERVACION DE EMBRIONES</subject><subject>CONSERVATION D'EMBRYON</subject><subject>CULTURE MEDIA</subject><subject>EMBRYO PRESERVATION</subject><subject>GANADO BOVINO</subject><subject>LINOLEIC ACID</subject><subject>MEDIO DE CULTIVO</subject><subject>MILIEU DE CULTURE</subject><subject>SUPERVIVENCIA</subject><subject>SURVIE</subject><subject>SURVIVAL</subject><subject>ZYGOTE</subject><subject>ZYGOTES</subject><issn>1341-7738</issn><issn>1347-5878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNotkM1LAzEQxRdRUKsX70LAm7A1X5uPo5RWK4oe1GuYzSaast1oslvRv96tFQbeHH7z3vCK4ozgKaGSX63WMU2JmBK9VxwRxmVZKan2_3ZSSsnUYXGc8wpjrhSWR0Wce-9sj6JHbehi64JFYENTQlsP69Chcfp3h5avDwi6ZtQFWrsmAIodykPahA2022uf4o_ryv4dvlyDPlLsBts6SKiOm9A59PP9FnuXT4oDD212p_86KV4W8-fZbXn_eLOcXd-XllOqSyZqqoXkkooKwIJtlLKEe6qZFVXNcI1BeM0bbmstGAhOgCnsKmop8bphk-Ji5zt-8jm43JtVHFI3RhrCORNSSUpG6nJH2RRzTs6bjxTWkL4NwWZbqNkWaogwRI_w-Q72EA28pZDN3RPRWmNcyapivzeaces</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science)</creator><creator>Kanamori, A</creator><creator>Sugisawa, K</creator><creator>Kimura, K</creator><creator>Hanada, A</creator><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>1999</creationdate><title>Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes</title><author>Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). 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(Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science)</creatorcontrib><creatorcontrib>Kanamori, A</creatorcontrib><creatorcontrib>Sugisawa, K</creatorcontrib><creatorcontrib>Kimura, K</creatorcontrib><creatorcontrib>Hanada, A</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of mammalian ova research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hochi, S. (Shinshu Univ., Ueda, Nagano (Japan). Faculty of Textile Science)</au><au>Kanamori, A</au><au>Sugisawa, K</au><au>Kimura, K</au><au>Hanada, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes</atitle><jtitle>Journal of mammalian ova research</jtitle><date>1999</date><risdate>1999</risdate><volume>16</volume><issue>1</issue><spage>19</spage><epage>22</epage><pages>19-22</pages><issn>1341-7738</issn><eissn>1347-5878</eissn><abstract>Bovine oocytes were matured and fertilized in a medium supplemented with or without 0.1% linoleic acid-albumin (LAA). The presumptive pronuclear-stage zygotes were frozen-thawed in the medium containing 1.5 M ethylene glycol with or without 0.1 M sucrose. The post-thaw survival of the zygotes was assessed by blastocyst development in vitro. In non-frozen control groups, 66 vs 64% (p=0.757) of the zygotes that were produced in the presence vs absence of LAA cleaved and 25 vs 27% (p=0.883) developed to blastocysts, respectively. After freezing and thawing, similar proportions (53-65%) of the zygotes appeared to be normal regardless of the LAA treatment in the IVM / IVF media (P≥0.775) and sucrose inclusion in the cryoprotective medium (P≥0.177). However, the post-thaw cleavage rate of LAA-treated zygotes (45-54%) was significantly higher (p<0.008) than that of non LAA-treated zygotes (23-29%). The post-thaw development of LAA-treated zygotes into blastocysts (14%) was also higher (p<0.003) than that of non LAA-treated zygotes (2%). Developmental kinetics and cell numbers of the resultant blastocysts were similar between frozen and non-frozen groups. These results suggest that presumptive pronuclear-stage bovine zygotes matured and fertilized in the presence of LAA are relatively tolerant to the process of freezing and thawing, although the post-thaw survival rate needs to be further improved.</abstract><cop>Fujisawa</cop><pub>Japan Science and Technology Agency</pub><doi>10.1274/jmor.16.19</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | ACIDE LINOLEIQUE ACIDO LINOLEICO ALBUMINAS ALBUMINE ALBUMINS BOVIN CATTLE CIGOTOS CONSERVACION DE EMBRIONES CONSERVATION D'EMBRYON CULTURE MEDIA EMBRYO PRESERVATION GANADO BOVINO LINOLEIC ACID MEDIO DE CULTIVO MILIEU DE CULTURE SUPERVIVENCIA SURVIE SURVIVAL ZYGOTE ZYGOTES |
| title | Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes |
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