Immobilization of [alpha]-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration
[alpha]-Amylase was immobilized on Dowex MAC-3 with 88 % yield and amyloglucosidase on Amberlite IRA-400 ion-exchange resin beads with 54 % yield by adsorption process. Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters. Optimum substrate conc...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2013-11, Vol.36 (11), p.1715 |
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| container_title | Bioprocess and biosystems engineering |
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| creator | Gupta, Kapish Jana, Asim Kumar Kumar, Sandeep Maiti, Mithu |
| description | [alpha]-Amylase was immobilized on Dowex MAC-3 with 88 % yield and amyloglucosidase on Amberlite IRA-400 ion-exchange resin beads with 54 % yield by adsorption process. Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters. Optimum substrate concentration and temperature were higher for immobilized enzymes. The thermal stability of the enzymes enhanced after the immobilization. Immobilized enzymes were used in the hydrolysis of the natural starch at high concentration (35 % w/v). The time required for liquefaction of starch to 10 dextrose equivalent (DE) and saccharification of liquefied starch to 96 DE increased. Immobilized enzymes showed the potential for use in starch hydrolysis as done in industry.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/s00449-013-0946-y |
| format | Article |
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Immobilized enzymes were characterized to measure the kinetic parameters and optimal operational parameters. Optimum substrate concentration and temperature were higher for immobilized enzymes. The thermal stability of the enzymes enhanced after the immobilization. Immobilized enzymes were used in the hydrolysis of the natural starch at high concentration (35 % w/v). The time required for liquefaction of starch to 10 dextrose equivalent (DE) and saccharification of liquefied starch to 96 DE increased. 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| language | eng |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Enzymes Hydrology Hydrolysis Ion exchange Liquefaction Starch |
| title | Immobilization of [alpha]-amylase and amyloglucosidase onto ion-exchange resin beads and hydrolysis of natural starch at high concentration |
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