Production and Characterization of Active Soluble Human [beta]1, 4-Galactosyltransferase in Escherichia coli as a Useful Catalyst in Synthesis of the Gal [beta]1->4 GlcNAc Linkage

An active and soluble human β1, 4-galactosyltransferase (β-GT) was produced in Escherichia coli using a maltose-binding protein fusion system. The purified recombinant β-GT has a Km value of 0.035mM for UDP-galactose and a Vmax of 643×103nmol/mg/h. The enzyme catalyzes the transfer of galactose from...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2001-01, Vol.91 (1), p.85
Hauptverfasser:	SHIBATANI, SHIGEO, FUJIYAMA, KAZUHITO, NISHIGUCHI, SUSUMU, SEKI, TATSUJI, MAEKAWA, YOSHIHIKO
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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