An instant measurement of oxidoreductase activity above 100°C by monitoring the absorbance change

A conventional absorbance monitoring method using a cuvette covered with a tight rubber cap was found to be applicable for measuring oxidoreductase activity at temperatures up to 115°C. Using this method, the optimal temperatures of the enzymes, including oxygen-sensitive enzymes from a hyperthermop...

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Veröffentlicht in:	Journal of Bioscience and Bioengineering 2004, Vol.97 (5), p.336-338
Hauptverfasser:	Imai, Takeo, Yasujima, Daisuke, Siddiqui, Masood Ahmed
Format:	Artikel
Sprache:	eng
Schlagworte:	anaerobic enzyme Biological and medical sciences Biotechnology dehydrogenase enzyme assay Fundamental and applied biological sciences. Psychology hyperthermophilic oxidoreductase
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A conventional absorbance monitoring method using a cuvette covered with a tight rubber cap was found to be applicable for measuring oxidoreductase activity at temperatures up to 115°C. Using this method, the optimal temperatures of the enzymes, including oxygen-sensitive enzymes from a hyperthermophilic archaeon Thermococcus profundus, were determined.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/S1389-1723(04)70215-7