Autofluorescence removal using a customized filter set

ABSTRACT Quantitative fluorescence microscopy is severely hindered by intrinsic autofluorescence (AF). Endogenous fluorescent molecules in tissue and cell samples emit fluorescence that often dominates signals from specific dyes. This makes AF removal critical to the development and practice of quan...

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Veröffentlicht in:	Microscopy research and technique 2013-10, Vol.76 (10), p.1007-1015
Hauptverfasser:	Pang, Zhengyu, barash, Eugene, Santamaria-Pang, Alberto, Sevinsky, Christopher, Li, Qing, Ginty, Fiona
Format:	Artikel
Sprache:	eng
Schlagworte:	autofluorescence removal filter set image subtraction immunofluorescence optical imaging
Online-Zugang:	Volltext
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