Quantitative Application for SDS–PAGE in a Biochemistry Lab

Quantitative Application for SDS–PAGE in a Biochemistry Lab

A variety of laboratory experiments have been published in this Journal describing the use of polyacrylamide gel electrophoresis to investigate different properties of proteins, including determination of the subunit composition of individual proteins and molar mass. The experiment described extends...

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Veröffentlicht in:Journal of chemical education 2013-09, Vol.90 (9), p.1255-1256
Hauptverfasser: Carter, John, Petersen, Brandon P, Printz, Sarah A, Sorey, Timothy L, Kroll, Todd T
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Biochemistry
Electrophoresis
Proteins
Science education
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container_issue 9
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container_title Journal of chemical education
container_volume 90
creator Carter, John
Petersen, Brandon P
Printz, Sarah A
Sorey, Timothy L
Kroll, Todd T
description A variety of laboratory experiments have been published in this Journal describing the use of polyacrylamide gel electrophoresis to investigate different properties of proteins, including determination of the subunit composition of individual proteins and molar mass. The experiment described extends the capabilities of this experimental technique and provides a mechanism to determine the concentration of a single protein from a heterogeneous mixture.
doi_str_mv 10.1021/ed300390j
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subjects Biochemistry
Electrophoresis
Proteins
Science education
title Quantitative Application for SDS–PAGE in a Biochemistry Lab
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